Research ExplorerPublicationsThe Phox2 homeodomain proteins are sufficient to promote the...

The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons

Standard

The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons. / Stanke, M; Junghans, D; Geissen, M; Goridis, C; Ernsberger, U; Rohrer, H.

In: DEVELOPMENT, Vol. 126, No. 18, 09.1999, p. 4087-4094.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Stanke, M, Junghans, D, Geissen, M, Goridis, C, Ernsberger, U & Rohrer, H 1999, 'The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons', DEVELOPMENT, vol. 126, no. 18, pp. 4087-4094. https://doi.org/10.1242/dev.126.18.4087

APA

Stanke, M., Junghans, D., Geissen, M., Goridis, C., Ernsberger, U., & Rohrer, H. (1999). The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons. DEVELOPMENT, 126(18), 4087-4094. https://doi.org/10.1242/dev.126.18.4087

Vancouver

Stanke M, Junghans D, Geissen M, Goridis C, Ernsberger U, Rohrer H. The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons. DEVELOPMENT. 1999 Sep;126(18):4087-4094. https://doi.org/10.1242/dev.126.18.4087

Bibtex

@article{209009852c2a4e0e89576983a49d0385,
title = "The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons",
abstract = "The development of sympathetic neurons is controlled by a network of transcriptional regulators, including the paired homeodomain proteins Phox2a and Phox2b. To understand the role of Phox2 proteins in more detail, the effect of Phox2 overexpression was analysed in the avian peripheral nervous system. Phox2a expression in neural crest cultures elicited a strong increase in the number of sympathoadrenergic cells. Expression of Phox2a in the chick embryo promoted the generation of additional neurons expressing the noradrenergic marker genes DBH and TH, pan-neuronal genes SCG10 and NF160 and cholinergic genes ChAT and VAChT. Phox2a-induced neurons were found in ectopic locations such as dorsal root ganglia and peripheral nerve. Sympathoadrenergic development could be elicited in cultures of E5 dorsal root ganglia, demonstrating the presence of Phox2a-responsive cells in non-autonomic peripheral ganglia. Phox2b induced ectopic neurons in the chick embryo in the same way as Phox2a. These results show that Phox2 proteins are sufficient to promote sympathetic neuron generation and control, directly or indirectly, the expression of a large number of genes characteristic for sympathetic neurons.",
keywords = "Animals, Carrier Proteins/genetics, Cell Differentiation/genetics, Chick Embryo, Choline O-Acetyltransferase/genetics, Culture Techniques, Dopamine beta-Hydroxylase/genetics, Drosophila Proteins, Embryo, Nonmammalian/virology, Embryonic Induction/genetics, Ganglia, Spinal/embryology, Gene Expression Regulation, Developmental, Homeodomain Proteins/genetics, Membrane Transport Proteins, Nerve Growth Factors/genetics, Nerve Tissue Proteins/genetics, Neurons/metabolism, Neurons, Afferent/metabolism, Proto-Oncogene Proteins/genetics, Proto-Oncogene Proteins c-ret, Quail/embryology, Receptor Protein-Tyrosine Kinases/genetics, Receptors, Adrenergic/metabolism, Retroviridae/genetics, Sympathetic Nervous System/cytology, Transcription Factors/genetics, Tyrosine 3-Monooxygenase/genetics, Vesicular Acetylcholine Transport Proteins, Vesicular Transport Proteins",
author = "M Stanke and D Junghans and M Geissen and C Goridis and U Ernsberger and H Rohrer",
year = "1999",
month = sep,
doi = "10.1242/dev.126.18.4087",
language = "English",
volume = "126",
pages = "4087--4094",
journal = "DEVELOPMENT",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "18",

}

RIS

TY - JOUR

T1 - The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons

AU - Stanke, M

AU - Junghans, D

AU - Geissen, M

AU - Goridis, C

AU - Ernsberger, U

AU - Rohrer, H

PY - 1999/9

Y1 - 1999/9

N2 - The development of sympathetic neurons is controlled by a network of transcriptional regulators, including the paired homeodomain proteins Phox2a and Phox2b. To understand the role of Phox2 proteins in more detail, the effect of Phox2 overexpression was analysed in the avian peripheral nervous system. Phox2a expression in neural crest cultures elicited a strong increase in the number of sympathoadrenergic cells. Expression of Phox2a in the chick embryo promoted the generation of additional neurons expressing the noradrenergic marker genes DBH and TH, pan-neuronal genes SCG10 and NF160 and cholinergic genes ChAT and VAChT. Phox2a-induced neurons were found in ectopic locations such as dorsal root ganglia and peripheral nerve. Sympathoadrenergic development could be elicited in cultures of E5 dorsal root ganglia, demonstrating the presence of Phox2a-responsive cells in non-autonomic peripheral ganglia. Phox2b induced ectopic neurons in the chick embryo in the same way as Phox2a. These results show that Phox2 proteins are sufficient to promote sympathetic neuron generation and control, directly or indirectly, the expression of a large number of genes characteristic for sympathetic neurons.

AB - The development of sympathetic neurons is controlled by a network of transcriptional regulators, including the paired homeodomain proteins Phox2a and Phox2b. To understand the role of Phox2 proteins in more detail, the effect of Phox2 overexpression was analysed in the avian peripheral nervous system. Phox2a expression in neural crest cultures elicited a strong increase in the number of sympathoadrenergic cells. Expression of Phox2a in the chick embryo promoted the generation of additional neurons expressing the noradrenergic marker genes DBH and TH, pan-neuronal genes SCG10 and NF160 and cholinergic genes ChAT and VAChT. Phox2a-induced neurons were found in ectopic locations such as dorsal root ganglia and peripheral nerve. Sympathoadrenergic development could be elicited in cultures of E5 dorsal root ganglia, demonstrating the presence of Phox2a-responsive cells in non-autonomic peripheral ganglia. Phox2b induced ectopic neurons in the chick embryo in the same way as Phox2a. These results show that Phox2 proteins are sufficient to promote sympathetic neuron generation and control, directly or indirectly, the expression of a large number of genes characteristic for sympathetic neurons.

KW - Animals

KW - Carrier Proteins/genetics

KW - Cell Differentiation/genetics

KW - Chick Embryo

KW - Choline O-Acetyltransferase/genetics

KW - Culture Techniques

KW - Dopamine beta-Hydroxylase/genetics

KW - Drosophila Proteins

KW - Embryo, Nonmammalian/virology

KW - Embryonic Induction/genetics

KW - Ganglia, Spinal/embryology

KW - Gene Expression Regulation, Developmental

KW - Homeodomain Proteins/genetics

KW - Membrane Transport Proteins

KW - Nerve Growth Factors/genetics

KW - Nerve Tissue Proteins/genetics

KW - Neurons/metabolism

KW - Neurons, Afferent/metabolism

KW - Proto-Oncogene Proteins/genetics

KW - Proto-Oncogene Proteins c-ret

KW - Quail/embryology

KW - Receptor Protein-Tyrosine Kinases/genetics

KW - Receptors, Adrenergic/metabolism

KW - Retroviridae/genetics

KW - Sympathetic Nervous System/cytology

KW - Transcription Factors/genetics

KW - Tyrosine 3-Monooxygenase/genetics

KW - Vesicular Acetylcholine Transport Proteins

KW - Vesicular Transport Proteins

U2 - 10.1242/dev.126.18.4087

DO - 10.1242/dev.126.18.4087

M3 - SCORING: Journal article

C2 - 10457017

VL - 126

SP - 4087

EP - 4094

JO - DEVELOPMENT

JF - DEVELOPMENT

SN - 0950-1991

IS - 18

ER -