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The microRNA-200 family--a potential diagnostic marker in hepatocellular carcinoma?

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The microRNA-200 family--a potential diagnostic marker in hepatocellular carcinoma? / Dhayat, Sameer A; Mardin, Wolf A; Köhler, Gabriele; Bahde, Ralf; Vowinkel, Thorsten; Wolters, Heiner; Senninger, Norbert; Haier, Jörg; Mees, Soeren T.

In: J SURG ONCOL, Vol. 110, No. 4, 09.2014, p. 430-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Dhayat, SA, Mardin, WA, Köhler, G, Bahde, R, Vowinkel, T, Wolters, H, Senninger, N, Haier, J & Mees, ST 2014, 'The microRNA-200 family--a potential diagnostic marker in hepatocellular carcinoma?', J SURG ONCOL, vol. 110, no. 4, pp. 430-8. https://doi.org/10.1002/jso.23668

APA

Dhayat, S. A., Mardin, W. A., Köhler, G., Bahde, R., Vowinkel, T., Wolters, H., Senninger, N., Haier, J., & Mees, S. T. (2014). The microRNA-200 family--a potential diagnostic marker in hepatocellular carcinoma? J SURG ONCOL, 110(4), 430-8. https://doi.org/10.1002/jso.23668

Vancouver

Dhayat SA, Mardin WA, Köhler G, Bahde R, Vowinkel T, Wolters H et al. The microRNA-200 family--a potential diagnostic marker in hepatocellular carcinoma? J SURG ONCOL. 2014 Sep;110(4):430-8. https://doi.org/10.1002/jso.23668

Bibtex

@article{458bcacfe9b94e00b916af4b55f8d15e,
title = "The microRNA-200 family--a potential diagnostic marker in hepatocellular carcinoma?",
abstract = "BACKGROUND: Hepatocellular carcinoma (HCC) represents the main cause of death among patients with cirrhotic liver disease, but little is known about mechanisms of cirrhosis associated carcinogenesis. We investigated the diagnostic impact of microRNA-200 (miR-200) family members as important epigenetic regulators of epithelial-mesenchymal transition (EMT) to differentiate between patients with HCC and liver cirrhosis.METHODS: Expression of the miR-200 family was investigated by qRT-PCR in specimens of HCC patients with and without cirrhosis. Benign specimens with and without cirrhosis served as controls. Expression of the EMT markers ZEB-1, E-cadherin and vimentin was examined using immunohistochemistry.RESULTS: MiR-200a and miR-200b were significantly downregulated in HCC (miR-200a: -40.1% (P = 0.0002); miR-200b: -52.3% (P = 0.0002)), and in HCC cirrhotic tissue (miR-200a: -40.2% (P = 0.004); miR-200b: -51.1% (P = 0.007)) compared to liver cirrhosis. Spearman's Rho analysis revealed a significant negative correlation of miR-200a and miR-200b to the expression of the mesenchymal markers Vimentin (P < 0.007) and ZEB-1 (P < 0.0005) and a significant positive correlation to the epithelial marker E-cadherin (P < 0.0002).CONCLUSIONS: MiR-200 family members and their targets are significantly deregulated in HCC and liver cirrhosis. The miR-200 family is able to distinguish between cirrhotic and HCC tissue and could serve as an early marker for cirrhosis-associated HCC.",
keywords = "Adult, Aged, Aged, 80 and over, Biomarkers, Tumor, Cadherins, Carcinoma, Hepatocellular, Epithelial-Mesenchymal Transition, Female, Homeodomain Proteins, Humans, Immunohistochemistry, Liver, Liver Cirrhosis, Liver Neoplasms, Male, MicroRNAs, Middle Aged, Transcription Factors",
author = "Dhayat, {Sameer A} and Mardin, {Wolf A} and Gabriele K{\"o}hler and Ralf Bahde and Thorsten Vowinkel and Heiner Wolters and Norbert Senninger and J{\"o}rg Haier and Mees, {Soeren T}",
note = "{\textcopyright} 2014 Wiley Periodicals, Inc.",
year = "2014",
month = sep,
doi = "10.1002/jso.23668",
language = "English",
volume = "110",
pages = "430--8",
journal = "J SURG ONCOL",
issn = "0022-4790",
publisher = "Wiley-Liss Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - The microRNA-200 family--a potential diagnostic marker in hepatocellular carcinoma?

AU - Dhayat, Sameer A

AU - Mardin, Wolf A

AU - Köhler, Gabriele

AU - Bahde, Ralf

AU - Vowinkel, Thorsten

AU - Wolters, Heiner

AU - Senninger, Norbert

AU - Haier, Jörg

AU - Mees, Soeren T

N1 - © 2014 Wiley Periodicals, Inc.

PY - 2014/9

Y1 - 2014/9

N2 - BACKGROUND: Hepatocellular carcinoma (HCC) represents the main cause of death among patients with cirrhotic liver disease, but little is known about mechanisms of cirrhosis associated carcinogenesis. We investigated the diagnostic impact of microRNA-200 (miR-200) family members as important epigenetic regulators of epithelial-mesenchymal transition (EMT) to differentiate between patients with HCC and liver cirrhosis.METHODS: Expression of the miR-200 family was investigated by qRT-PCR in specimens of HCC patients with and without cirrhosis. Benign specimens with and without cirrhosis served as controls. Expression of the EMT markers ZEB-1, E-cadherin and vimentin was examined using immunohistochemistry.RESULTS: MiR-200a and miR-200b were significantly downregulated in HCC (miR-200a: -40.1% (P = 0.0002); miR-200b: -52.3% (P = 0.0002)), and in HCC cirrhotic tissue (miR-200a: -40.2% (P = 0.004); miR-200b: -51.1% (P = 0.007)) compared to liver cirrhosis. Spearman's Rho analysis revealed a significant negative correlation of miR-200a and miR-200b to the expression of the mesenchymal markers Vimentin (P < 0.007) and ZEB-1 (P < 0.0005) and a significant positive correlation to the epithelial marker E-cadherin (P < 0.0002).CONCLUSIONS: MiR-200 family members and their targets are significantly deregulated in HCC and liver cirrhosis. The miR-200 family is able to distinguish between cirrhotic and HCC tissue and could serve as an early marker for cirrhosis-associated HCC.

AB - BACKGROUND: Hepatocellular carcinoma (HCC) represents the main cause of death among patients with cirrhotic liver disease, but little is known about mechanisms of cirrhosis associated carcinogenesis. We investigated the diagnostic impact of microRNA-200 (miR-200) family members as important epigenetic regulators of epithelial-mesenchymal transition (EMT) to differentiate between patients with HCC and liver cirrhosis.METHODS: Expression of the miR-200 family was investigated by qRT-PCR in specimens of HCC patients with and without cirrhosis. Benign specimens with and without cirrhosis served as controls. Expression of the EMT markers ZEB-1, E-cadherin and vimentin was examined using immunohistochemistry.RESULTS: MiR-200a and miR-200b were significantly downregulated in HCC (miR-200a: -40.1% (P = 0.0002); miR-200b: -52.3% (P = 0.0002)), and in HCC cirrhotic tissue (miR-200a: -40.2% (P = 0.004); miR-200b: -51.1% (P = 0.007)) compared to liver cirrhosis. Spearman's Rho analysis revealed a significant negative correlation of miR-200a and miR-200b to the expression of the mesenchymal markers Vimentin (P < 0.007) and ZEB-1 (P < 0.0005) and a significant positive correlation to the epithelial marker E-cadherin (P < 0.0002).CONCLUSIONS: MiR-200 family members and their targets are significantly deregulated in HCC and liver cirrhosis. The miR-200 family is able to distinguish between cirrhotic and HCC tissue and could serve as an early marker for cirrhosis-associated HCC.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Biomarkers, Tumor

KW - Cadherins

KW - Carcinoma, Hepatocellular

KW - Epithelial-Mesenchymal Transition

KW - Female

KW - Homeodomain Proteins

KW - Humans

KW - Immunohistochemistry

KW - Liver

KW - Liver Cirrhosis

KW - Liver Neoplasms

KW - Male

KW - MicroRNAs

KW - Middle Aged

KW - Transcription Factors

U2 - 10.1002/jso.23668

DO - 10.1002/jso.23668

M3 - SCORING: Journal article

C2 - 24895326

VL - 110

SP - 430

EP - 438

JO - J SURG ONCOL

JF - J SURG ONCOL

SN - 0022-4790

IS - 4

ER -