The microRNA bantam regulates a developmental transition in epithelial cells that restricts sensory dendrite growth
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The microRNA bantam regulates a developmental transition in epithelial cells that restricts sensory dendrite growth. / Jiang, Nan; Soba, Peter; Parker, Edward; Kim, Charles C; Parrish, Jay Z.
In: DEVELOPMENT, Vol. 141, No. 13, 01.07.2014, p. 2657-68.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - The microRNA bantam regulates a developmental transition in epithelial cells that restricts sensory dendrite growth
AU - Jiang, Nan
AU - Soba, Peter
AU - Parker, Edward
AU - Kim, Charles C
AU - Parrish, Jay Z
N1 - © 2014. Published by The Company of Biologists Ltd.
PY - 2014/7/1
Y1 - 2014/7/1
N2 - As animals grow, many early born structures grow by cell expansion rather than cell addition; thus growth of distinct structures must be coordinated to maintain proportionality. This phenomenon is particularly widespread in the nervous system, with dendrite arbors of many neurons expanding in concert with their substrate to sustain connectivity and maintain receptive field coverage as animals grow. After rapidly growing to establish body wall coverage, dendrites of Drosophila class IV dendrite arborization (C4da) neurons grow synchronously with their substrate, the body wall epithelium, providing a system to study how proportionality is maintained during animal growth. Here, we show that the microRNA bantam (ban) ensures coordinated growth of C4da dendrites and the epithelium through regulation of epithelial endoreplication, a modified cell cycle that entails genome amplification without cell division. In Drosophila larvae, epithelial endoreplication leads to progressive changes in dendrite-extracellular matrix (ECM) and dendrite-epithelium contacts, coupling dendrite/substrate expansion and restricting dendrite growth beyond established boundaries. Moreover, changes in epithelial expression of cell adhesion molecules, including the beta-integrin myospheroid (mys), accompany this developmental transition. Finally, endoreplication and the accompanying changes in epithelial mys expression are required to constrain late-stage dendrite growth and structural plasticity. Hence, modulating epithelium-ECM attachment probably influences substrate permissivity for dendrite growth and contributes to the dendrite-substrate coupling that ensures proportional expansion of the two cell types.
AB - As animals grow, many early born structures grow by cell expansion rather than cell addition; thus growth of distinct structures must be coordinated to maintain proportionality. This phenomenon is particularly widespread in the nervous system, with dendrite arbors of many neurons expanding in concert with their substrate to sustain connectivity and maintain receptive field coverage as animals grow. After rapidly growing to establish body wall coverage, dendrites of Drosophila class IV dendrite arborization (C4da) neurons grow synchronously with their substrate, the body wall epithelium, providing a system to study how proportionality is maintained during animal growth. Here, we show that the microRNA bantam (ban) ensures coordinated growth of C4da dendrites and the epithelium through regulation of epithelial endoreplication, a modified cell cycle that entails genome amplification without cell division. In Drosophila larvae, epithelial endoreplication leads to progressive changes in dendrite-extracellular matrix (ECM) and dendrite-epithelium contacts, coupling dendrite/substrate expansion and restricting dendrite growth beyond established boundaries. Moreover, changes in epithelial expression of cell adhesion molecules, including the beta-integrin myospheroid (mys), accompany this developmental transition. Finally, endoreplication and the accompanying changes in epithelial mys expression are required to constrain late-stage dendrite growth and structural plasticity. Hence, modulating epithelium-ECM attachment probably influences substrate permissivity for dendrite growth and contributes to the dendrite-substrate coupling that ensures proportional expansion of the two cell types.
KW - Analysis of Variance
KW - Animals
KW - Cell Enlargement
KW - Dendrites
KW - Drosophila
KW - Endoreduplication
KW - Epithelial Cells
KW - Flow Cytometry
KW - Immunohistochemistry
KW - MicroRNAs
KW - Microscopy, Electron, Transmission
KW - Sensory Receptor Cells
U2 - 10.1242/dev.107573
DO - 10.1242/dev.107573
M3 - SCORING: Journal article
C2 - 24924190
VL - 141
SP - 2657
EP - 2668
JO - DEVELOPMENT
JF - DEVELOPMENT
SN - 0950-1991
IS - 13
ER -