The Microbiome Activates CD4 T-cell-mediated Immunity to Compensate for Increased Intestinal Permeability
Standard
The Microbiome Activates CD4 T-cell-mediated Immunity to Compensate for Increased Intestinal Permeability. / Edelblum, Karen L; Sharon, Gil; Singh, Gurminder; Odenwald, Matthew A; Sailer, Anne; Cao, Severine; Ravens, Sarina; Thomsen, Irene; El Bissati, Kamal; McLeod, Rima; Dong, Chen; Gurbuxani, Sandeep; Prinz, Immo; Mazmanian, Sarkis K; Turner, Jerrold R.
In: CELL MOL GASTROENTER, Vol. 4, No. 2, 10.06.2017, p. 285-297.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - The Microbiome Activates CD4 T-cell-mediated Immunity to Compensate for Increased Intestinal Permeability
AU - Edelblum, Karen L
AU - Sharon, Gil
AU - Singh, Gurminder
AU - Odenwald, Matthew A
AU - Sailer, Anne
AU - Cao, Severine
AU - Ravens, Sarina
AU - Thomsen, Irene
AU - El Bissati, Kamal
AU - McLeod, Rima
AU - Dong, Chen
AU - Gurbuxani, Sandeep
AU - Prinz, Immo
AU - Mazmanian, Sarkis K
AU - Turner, Jerrold R
PY - 2017/6/10
Y1 - 2017/6/10
N2 - BACKGROUND & AIMS: Despite a prominent association, chronic intestinal barrier loss is insufficient to induce disease in human subjects or experimental animals. We hypothesized that compensatory mucosal immune activation might protect individuals with increased intestinal permeability from disease. We used a model in which intestinal barrier loss is triggered by intestinal epithelial-specific expression of constitutively active myosin light chain kinase (CA-MLCK). Here we asked whether constitutive tight junction barrier loss impacts susceptibility to enteric pathogens.METHODS: Acute or chronic Toxoplasma gondii or Salmonella typhimurium infection was assessed in CA-MLCK transgenic or wild-type mice. Germ-free mice or those lacking specific immune cell populations were used to investigate the effect of microbial-activated immunity on pathogen translocation in the context of increased intestinal permeability.RESULTS: Acute T gondii and S typhimurium translocation across the epithelial barrier was reduced in CA-MLCK mice. This protection was due to enhanced mucosal immune activation that required CD4+ T cells and interleukin 17A but not immunoglobulin A. The protective mucosal immune activation in CA-MLCK mice depended on segmented filamentous bacteria (SFB), because protection against early S typhimurium invasion was lost in germ-free CA-MLCK mice but could be restored by conventionalization with SFB-containing, not SFB-deficient, microbiota. In contrast, chronic S typhimurium infection was more severe in CA-MLCK mice, suggesting that despite activation of protective mucosal immunity, barrier defects ultimately result in enhanced disease progression.CONCLUSIONS: Increased epithelial tight junction permeability synergizes with commensal bacteria to promote intestinal CD4+ T-cell expansion and interleukin 17A production that limits enteric pathogen invasion.
AB - BACKGROUND & AIMS: Despite a prominent association, chronic intestinal barrier loss is insufficient to induce disease in human subjects or experimental animals. We hypothesized that compensatory mucosal immune activation might protect individuals with increased intestinal permeability from disease. We used a model in which intestinal barrier loss is triggered by intestinal epithelial-specific expression of constitutively active myosin light chain kinase (CA-MLCK). Here we asked whether constitutive tight junction barrier loss impacts susceptibility to enteric pathogens.METHODS: Acute or chronic Toxoplasma gondii or Salmonella typhimurium infection was assessed in CA-MLCK transgenic or wild-type mice. Germ-free mice or those lacking specific immune cell populations were used to investigate the effect of microbial-activated immunity on pathogen translocation in the context of increased intestinal permeability.RESULTS: Acute T gondii and S typhimurium translocation across the epithelial barrier was reduced in CA-MLCK mice. This protection was due to enhanced mucosal immune activation that required CD4+ T cells and interleukin 17A but not immunoglobulin A. The protective mucosal immune activation in CA-MLCK mice depended on segmented filamentous bacteria (SFB), because protection against early S typhimurium invasion was lost in germ-free CA-MLCK mice but could be restored by conventionalization with SFB-containing, not SFB-deficient, microbiota. In contrast, chronic S typhimurium infection was more severe in CA-MLCK mice, suggesting that despite activation of protective mucosal immunity, barrier defects ultimately result in enhanced disease progression.CONCLUSIONS: Increased epithelial tight junction permeability synergizes with commensal bacteria to promote intestinal CD4+ T-cell expansion and interleukin 17A production that limits enteric pathogen invasion.
U2 - 10.1016/j.jcmgh.2017.06.001
DO - 10.1016/j.jcmgh.2017.06.001
M3 - SCORING: Journal article
C2 - 28795125
VL - 4
SP - 285
EP - 297
JO - CELL MOL GASTROENTER
JF - CELL MOL GASTROENTER
SN - 2352-345X
IS - 2
ER -