The magnitude and breadth of hepatitis C virus-specific CD8+ T cells depend on absolute CD4+ T-cell count in individuals coinfected with HIV-1
Standard
The magnitude and breadth of hepatitis C virus-specific CD8+ T cells depend on absolute CD4+ T-cell count in individuals coinfected with HIV-1. / Kim, Arthur Y; Lauer, Georg M; Ouchi, Kei; Addo, Marylyn M; Lucas, Michaela; Schulze Zur Wiesch, Julian; Timm, Joerg; Boczanowski, Melinda; Duncan, Jared E; Wurcel, Alysse G; Casson, Deborah; Chung, Raymond T; Draenert, Rika; Klenerman, Paul; Walker, Bruce D.
In: BLOOD, Vol. 105, No. 3, 01.02.2005, p. 1170-8.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - The magnitude and breadth of hepatitis C virus-specific CD8+ T cells depend on absolute CD4+ T-cell count in individuals coinfected with HIV-1
AU - Kim, Arthur Y
AU - Lauer, Georg M
AU - Ouchi, Kei
AU - Addo, Marylyn M
AU - Lucas, Michaela
AU - Schulze Zur Wiesch, Julian
AU - Timm, Joerg
AU - Boczanowski, Melinda
AU - Duncan, Jared E
AU - Wurcel, Alysse G
AU - Casson, Deborah
AU - Chung, Raymond T
AU - Draenert, Rika
AU - Klenerman, Paul
AU - Walker, Bruce D
PY - 2005/2/1
Y1 - 2005/2/1
N2 - CD8(+) T-cell responses are an essential antiviral host defense in persistent viral infections, and their sustained effectiveness is thought to be critically dependent on CD4(+) T-helper cells. To determine the relationship between HIV-1-induced CD4(+) T-cell depletion and hepatitis C virus (HCV)-specific CD8(+) T-cell responses during viral persistence, we studied 103 persons positive for HCV, 74 coinfected with HIV-1. CD8(+) T-cell responses to the entire HCV polyprotein were determined by using an interferon-gamma enzyme-linked immunospot (ELISpot) assay. Although HIV-1 infection by itself was not associated with a diminished HCV-specific response, HIV-1-associated CD4(+) depletion was associated with significantly lower HCV-specific CD8(+) T cells (R = 0.48, P < .0001). In contrast, declining CD4(+) counts over the same range were not associated with diminished Epstein-Barr virus (EBV)- (R = 0.19, P = .31) or HIV-1-specific (R = -0.13, P = .60) CD8(+) T-cell responses in persons infected with all viruses. These data indicate that frequencies of circulating HCV-specific CD8(+) T-cell responses are sensitive to absolute CD4(+) T-cell counts and provide a possible explanation for the accelerated HCV disease course in persons coinfected with HIV-1 and HCV.
AB - CD8(+) T-cell responses are an essential antiviral host defense in persistent viral infections, and their sustained effectiveness is thought to be critically dependent on CD4(+) T-helper cells. To determine the relationship between HIV-1-induced CD4(+) T-cell depletion and hepatitis C virus (HCV)-specific CD8(+) T-cell responses during viral persistence, we studied 103 persons positive for HCV, 74 coinfected with HIV-1. CD8(+) T-cell responses to the entire HCV polyprotein were determined by using an interferon-gamma enzyme-linked immunospot (ELISpot) assay. Although HIV-1 infection by itself was not associated with a diminished HCV-specific response, HIV-1-associated CD4(+) depletion was associated with significantly lower HCV-specific CD8(+) T cells (R = 0.48, P < .0001). In contrast, declining CD4(+) counts over the same range were not associated with diminished Epstein-Barr virus (EBV)- (R = 0.19, P = .31) or HIV-1-specific (R = -0.13, P = .60) CD8(+) T-cell responses in persons infected with all viruses. These data indicate that frequencies of circulating HCV-specific CD8(+) T-cell responses are sensitive to absolute CD4(+) T-cell counts and provide a possible explanation for the accelerated HCV disease course in persons coinfected with HIV-1 and HCV.
KW - Acquired Immunodeficiency Syndrome/immunology
KW - Adult
KW - Biopsy
KW - CD4 Lymphocyte Count
KW - CD8-Positive T-Lymphocytes/immunology
KW - Cytokines/analysis
KW - Female
KW - Genotype
KW - HIV Infections/immunology
KW - HIV-1/genetics
KW - Hepacivirus/genetics
KW - Hepatitis C/immunology
KW - Histocompatibility Testing
KW - Humans
KW - Male
KW - Middle Aged
KW - RNA, Viral/blood
KW - Viral Load
U2 - 10.1182/blood-2004-06-2336
DO - 10.1182/blood-2004-06-2336
M3 - SCORING: Journal article
C2 - 15459014
VL - 105
SP - 1170
EP - 1178
JO - BLOOD
JF - BLOOD
SN - 0006-4971
IS - 3
ER -