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The GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium.

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The GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium. / Jaschke, Alexander; Chung, Bomee; Hesse, Deike; Kluge, Reinhart; Zahn, Claudia; Moser, Markus; Petzke, Klaus-Jürgen; Brigelius-Flohé, Regina; Puchkov, Dmytro; Koepsell, Hermann; Heeren, Jörg; Joost, Hans-Georg; Schürmann, Annette.

In: HUM MOL GENET, Vol. 21, No. 14, 14, 2012, p. 3128-3142.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Jaschke, A, Chung, B, Hesse, D, Kluge, R, Zahn, C, Moser, M, Petzke, K-J, Brigelius-Flohé, R, Puchkov, D, Koepsell, H, Heeren, J, Joost, H-G & Schürmann, A 2012, 'The GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium.', HUM MOL GENET, vol. 21, no. 14, 14, pp. 3128-3142. <http://www.ncbi.nlm.nih.gov/pubmed/22505585?dopt=Citation>

APA

Jaschke, A., Chung, B., Hesse, D., Kluge, R., Zahn, C., Moser, M., Petzke, K-J., Brigelius-Flohé, R., Puchkov, D., Koepsell, H., Heeren, J., Joost, H-G., & Schürmann, A. (2012). The GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium. HUM MOL GENET, 21(14), 3128-3142. [14]. http://www.ncbi.nlm.nih.gov/pubmed/22505585?dopt=Citation

Vancouver

Jaschke A, Chung B, Hesse D, Kluge R, Zahn C, Moser M et al. The GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium. HUM MOL GENET. 2012;21(14):3128-3142. 14.

Bibtex

@article{919e7213b53a4c5badb8e307a2b8c973,
title = "The GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium.",
abstract = "The uptake and processing of dietary lipids by the small intestine is a multistep process that involves several steps including vesicular and protein transport. The GTPase ADP-ribosylation factor-related protein 1 (ARFRP1) controls the ARF-like 1 (ARL1)-mediated Golgi recruitment of GRIP domain proteins which in turn bind several Rab-GTPases. Here, we describe the essential role of ARFRP1 and its interaction with Rab2 in the assembly and lipidation of chylomicrons in the intestinal epithelium. Mice lacking Arfrp1 specifically in the intestine (Arfrp1(vil-/-)) exhibit an early post-natal growth retardation with reduced plasma triacylglycerol and free fatty acid concentrations. Arfrp1(vil-/-) enterocytes as well as Arfrp1 mRNA depleted Caco-2 cells absorbed fatty acids normally but secreted chylomicrons with a markedly reduced triacylglycerol content. In addition, the release of apolipoprotein A-I (ApoA-I) was dramatically decreased, and ApoA-I accumulated in the Arfrp1(vil-/-) epithelium, where it predominantly co-localized with Rab2. The release of chylomicrons from Caco-2 was markedly reduced after the suppression of Rab2, ARL1 and Golgin-245. Thus, the GTPase ARFRP1 and its downstream proteins are required for the lipidation of chylo-microns and the assembly of ApoA-I to these particles in the Golgi of intestinal epithelial cells.",
keywords = "Animals, Humans, Mice, Mice, Knockout, Lipid Metabolism, Protein Transport, Protein Binding, GTP Phosphohydrolases/genetics/*metabolism, ADP-Ribosylation Factors/genetics/*metabolism, Apolipoprotein A-I/metabolism, Chylomicrons/*metabolism, Golgi Apparatus/*enzymology/metabolism, Intestinal Mucosa/*enzymology/metabolism, rab2 GTP-Binding Protein/genetics/metabolism, Animals, Humans, Mice, Mice, Knockout, Lipid Metabolism, Protein Transport, Protein Binding, GTP Phosphohydrolases/genetics/*metabolism, ADP-Ribosylation Factors/genetics/*metabolism, Apolipoprotein A-I/metabolism, Chylomicrons/*metabolism, Golgi Apparatus/*enzymology/metabolism, Intestinal Mucosa/*enzymology/metabolism, rab2 GTP-Binding Protein/genetics/metabolism",
author = "Alexander Jaschke and Bomee Chung and Deike Hesse and Reinhart Kluge and Claudia Zahn and Markus Moser and Klaus-J{\"u}rgen Petzke and Regina Brigelius-Floh{\'e} and Dmytro Puchkov and Hermann Koepsell and J{\"o}rg Heeren and Hans-Georg Joost and Annette Sch{\"u}rmann",
year = "2012",
language = "English",
volume = "21",
pages = "3128--3142",
journal = "HUM MOL GENET",
issn = "0964-6906",
publisher = "Oxford University Press",
number = "14",

}

RIS

TY - JOUR

T1 - The GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium.

AU - Jaschke, Alexander

AU - Chung, Bomee

AU - Hesse, Deike

AU - Kluge, Reinhart

AU - Zahn, Claudia

AU - Moser, Markus

AU - Petzke, Klaus-Jürgen

AU - Brigelius-Flohé, Regina

AU - Puchkov, Dmytro

AU - Koepsell, Hermann

AU - Heeren, Jörg

AU - Joost, Hans-Georg

AU - Schürmann, Annette

PY - 2012

Y1 - 2012

N2 - The uptake and processing of dietary lipids by the small intestine is a multistep process that involves several steps including vesicular and protein transport. The GTPase ADP-ribosylation factor-related protein 1 (ARFRP1) controls the ARF-like 1 (ARL1)-mediated Golgi recruitment of GRIP domain proteins which in turn bind several Rab-GTPases. Here, we describe the essential role of ARFRP1 and its interaction with Rab2 in the assembly and lipidation of chylomicrons in the intestinal epithelium. Mice lacking Arfrp1 specifically in the intestine (Arfrp1(vil-/-)) exhibit an early post-natal growth retardation with reduced plasma triacylglycerol and free fatty acid concentrations. Arfrp1(vil-/-) enterocytes as well as Arfrp1 mRNA depleted Caco-2 cells absorbed fatty acids normally but secreted chylomicrons with a markedly reduced triacylglycerol content. In addition, the release of apolipoprotein A-I (ApoA-I) was dramatically decreased, and ApoA-I accumulated in the Arfrp1(vil-/-) epithelium, where it predominantly co-localized with Rab2. The release of chylomicrons from Caco-2 was markedly reduced after the suppression of Rab2, ARL1 and Golgin-245. Thus, the GTPase ARFRP1 and its downstream proteins are required for the lipidation of chylo-microns and the assembly of ApoA-I to these particles in the Golgi of intestinal epithelial cells.

AB - The uptake and processing of dietary lipids by the small intestine is a multistep process that involves several steps including vesicular and protein transport. The GTPase ADP-ribosylation factor-related protein 1 (ARFRP1) controls the ARF-like 1 (ARL1)-mediated Golgi recruitment of GRIP domain proteins which in turn bind several Rab-GTPases. Here, we describe the essential role of ARFRP1 and its interaction with Rab2 in the assembly and lipidation of chylomicrons in the intestinal epithelium. Mice lacking Arfrp1 specifically in the intestine (Arfrp1(vil-/-)) exhibit an early post-natal growth retardation with reduced plasma triacylglycerol and free fatty acid concentrations. Arfrp1(vil-/-) enterocytes as well as Arfrp1 mRNA depleted Caco-2 cells absorbed fatty acids normally but secreted chylomicrons with a markedly reduced triacylglycerol content. In addition, the release of apolipoprotein A-I (ApoA-I) was dramatically decreased, and ApoA-I accumulated in the Arfrp1(vil-/-) epithelium, where it predominantly co-localized with Rab2. The release of chylomicrons from Caco-2 was markedly reduced after the suppression of Rab2, ARL1 and Golgin-245. Thus, the GTPase ARFRP1 and its downstream proteins are required for the lipidation of chylo-microns and the assembly of ApoA-I to these particles in the Golgi of intestinal epithelial cells.

KW - Animals

KW - Humans

KW - Mice

KW - Mice, Knockout

KW - Lipid Metabolism

KW - Protein Transport

KW - Protein Binding

KW - GTP Phosphohydrolases/genetics/metabolism

KW - ADP-Ribosylation Factors/genetics/metabolism

KW - Apolipoprotein A-I/metabolism

KW - Chylomicrons/metabolism

KW - Golgi Apparatus/enzymology/metabolism

KW - Intestinal Mucosa/enzymology/metabolism

KW - rab2 GTP-Binding Protein/genetics/metabolism

KW - Animals

KW - Humans

KW - Mice

KW - Mice, Knockout

KW - Lipid Metabolism

KW - Protein Transport

KW - Protein Binding

KW - GTP Phosphohydrolases/genetics/metabolism

KW - ADP-Ribosylation Factors/genetics/metabolism

KW - Apolipoprotein A-I/metabolism

KW - Chylomicrons/metabolism

KW - Golgi Apparatus/enzymology/metabolism

KW - Intestinal Mucosa/enzymology/metabolism

KW - rab2 GTP-Binding Protein/genetics/metabolism

M3 - SCORING: Journal article

VL - 21

SP - 3128

EP - 3142

JO - HUM MOL GENET

JF - HUM MOL GENET

SN - 0964-6906

IS - 14

M1 - 14

ER -