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The equilibrium of XIAP and Smac/DIABLO expression is gradually deranged during the development and progression of testicular germ cell tumours.

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The equilibrium of XIAP and Smac/DIABLO expression is gradually deranged during the development and progression of testicular germ cell tumours. / Kempkensteffen, Carsten; Jäger, Tobias; Bub, Johannes; Weikert, Steffen; Hinz, Stefan; Christoph, Frank; Krause, Hans; Schostak, Martin; Miller, Kurt; Schrader, Mark.

In: INT J ANDROL, Vol. 30, No. 5, 5, 2007, p. 476-483.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kempkensteffen, C, Jäger, T, Bub, J, Weikert, S, Hinz, S, Christoph, F, Krause, H, Schostak, M, Miller, K & Schrader, M 2007, 'The equilibrium of XIAP and Smac/DIABLO expression is gradually deranged during the development and progression of testicular germ cell tumours.', INT J ANDROL, vol. 30, no. 5, 5, pp. 476-483. <http://www.ncbi.nlm.nih.gov/pubmed/17298543?dopt=Citation>

APA

Kempkensteffen, C., Jäger, T., Bub, J., Weikert, S., Hinz, S., Christoph, F., Krause, H., Schostak, M., Miller, K., & Schrader, M. (2007). The equilibrium of XIAP and Smac/DIABLO expression is gradually deranged during the development and progression of testicular germ cell tumours. INT J ANDROL, 30(5), 476-483. [5]. http://www.ncbi.nlm.nih.gov/pubmed/17298543?dopt=Citation

Vancouver

Kempkensteffen C, Jäger T, Bub J, Weikert S, Hinz S, Christoph F et al. The equilibrium of XIAP and Smac/DIABLO expression is gradually deranged during the development and progression of testicular germ cell tumours. INT J ANDROL. 2007;30(5):476-483. 5.

Bibtex

@article{b261b79d8aaf410e8fb0037e78205f6b,
title = "The equilibrium of XIAP and Smac/DIABLO expression is gradually deranged during the development and progression of testicular germ cell tumours.",
abstract = "Overexpression of the inhibitor of apoptosis protein (IAP) XIAP (BIRC4) and downregulation of its antagonist Smac/DIABLO (DIABLO) are associated with the onset and progression of various malignancies. In this study, real-time RT-PCR was used to quantify the mRNA expression of XIAP and Smac/DIABLO in normal testicular tissue (n = 19), testicular carcinoma in situ (CIS; n = 4), testicular seminomas (n = 64) and non-seminomatous germ cell tumours (NSGCT; n = 35). XIAP and Smac/DIABLO were commonly expressed in normal and malignant testicular tissue with no apparent differences in XIAP mRNA levels among the histologic subgroups. Smac/DIABLO levels, on the other hand, gradually decreased from normal testicular tissue to CIS and seminomas and finally to NSGCT (p <0.001). An inverse trend was observed when calculating the XIAP-to-Smac/DIABLO ratio (p <0.001). This ratio differed when comparing normal testicular tissue with CIS (p = 0.014), seminomas (p <0.001) and NSGCT (p <0.001) and when comparing seminomas with NSGCT (p = 0.002), whereas no such difference was observed between CIS and seminomas (p = 0.302). TGCT patients dichotomized by the overall median XIAP-to-Smac/DIABLO ratio were more likely to present with a high ratio in clinical stage (CS) III than in CS I or II (p = 0.034). These data indicate that the balance of mRNA expression between XIAP and Smac/DIABLO is altered in favour of antiapoptotic XIAP during the development and progression of TGCT. Thus the expression of proapoptotic Smac/DIABLO is lowest in NSGCT and stage III tumours.",
author = "Carsten Kempkensteffen and Tobias J{\"a}ger and Johannes Bub and Steffen Weikert and Stefan Hinz and Frank Christoph and Hans Krause and Martin Schostak and Kurt Miller and Mark Schrader",
year = "2007",
language = "Deutsch",
volume = "30",
pages = "476--483",
number = "5",

}

RIS

TY - JOUR

T1 - The equilibrium of XIAP and Smac/DIABLO expression is gradually deranged during the development and progression of testicular germ cell tumours.

AU - Kempkensteffen, Carsten

AU - Jäger, Tobias

AU - Bub, Johannes

AU - Weikert, Steffen

AU - Hinz, Stefan

AU - Christoph, Frank

AU - Krause, Hans

AU - Schostak, Martin

AU - Miller, Kurt

AU - Schrader, Mark

PY - 2007

Y1 - 2007

N2 - Overexpression of the inhibitor of apoptosis protein (IAP) XIAP (BIRC4) and downregulation of its antagonist Smac/DIABLO (DIABLO) are associated with the onset and progression of various malignancies. In this study, real-time RT-PCR was used to quantify the mRNA expression of XIAP and Smac/DIABLO in normal testicular tissue (n = 19), testicular carcinoma in situ (CIS; n = 4), testicular seminomas (n = 64) and non-seminomatous germ cell tumours (NSGCT; n = 35). XIAP and Smac/DIABLO were commonly expressed in normal and malignant testicular tissue with no apparent differences in XIAP mRNA levels among the histologic subgroups. Smac/DIABLO levels, on the other hand, gradually decreased from normal testicular tissue to CIS and seminomas and finally to NSGCT (p <0.001). An inverse trend was observed when calculating the XIAP-to-Smac/DIABLO ratio (p <0.001). This ratio differed when comparing normal testicular tissue with CIS (p = 0.014), seminomas (p <0.001) and NSGCT (p <0.001) and when comparing seminomas with NSGCT (p = 0.002), whereas no such difference was observed between CIS and seminomas (p = 0.302). TGCT patients dichotomized by the overall median XIAP-to-Smac/DIABLO ratio were more likely to present with a high ratio in clinical stage (CS) III than in CS I or II (p = 0.034). These data indicate that the balance of mRNA expression between XIAP and Smac/DIABLO is altered in favour of antiapoptotic XIAP during the development and progression of TGCT. Thus the expression of proapoptotic Smac/DIABLO is lowest in NSGCT and stage III tumours.

AB - Overexpression of the inhibitor of apoptosis protein (IAP) XIAP (BIRC4) and downregulation of its antagonist Smac/DIABLO (DIABLO) are associated with the onset and progression of various malignancies. In this study, real-time RT-PCR was used to quantify the mRNA expression of XIAP and Smac/DIABLO in normal testicular tissue (n = 19), testicular carcinoma in situ (CIS; n = 4), testicular seminomas (n = 64) and non-seminomatous germ cell tumours (NSGCT; n = 35). XIAP and Smac/DIABLO were commonly expressed in normal and malignant testicular tissue with no apparent differences in XIAP mRNA levels among the histologic subgroups. Smac/DIABLO levels, on the other hand, gradually decreased from normal testicular tissue to CIS and seminomas and finally to NSGCT (p <0.001). An inverse trend was observed when calculating the XIAP-to-Smac/DIABLO ratio (p <0.001). This ratio differed when comparing normal testicular tissue with CIS (p = 0.014), seminomas (p <0.001) and NSGCT (p <0.001) and when comparing seminomas with NSGCT (p = 0.002), whereas no such difference was observed between CIS and seminomas (p = 0.302). TGCT patients dichotomized by the overall median XIAP-to-Smac/DIABLO ratio were more likely to present with a high ratio in clinical stage (CS) III than in CS I or II (p = 0.034). These data indicate that the balance of mRNA expression between XIAP and Smac/DIABLO is altered in favour of antiapoptotic XIAP during the development and progression of TGCT. Thus the expression of proapoptotic Smac/DIABLO is lowest in NSGCT and stage III tumours.

M3 - SCORING: Zeitschriftenaufsatz

VL - 30

SP - 476

EP - 483

IS - 5

M1 - 5

ER -