The dual pro-inflammatory and bone-protective role of calcitonin gene-related peptide alpha in age-related osteoarthritis

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The dual pro-inflammatory and bone-protective role of calcitonin gene-related peptide alpha in age-related osteoarthritis. / Hildebrandt, Alexander; Dietrich, Tamara; Weber, Jérôme; Günderoth, Mara Meyer; Zhou, Sijia; Fleckenstein, Florian N; Jiang, Shan; Winkler, Tobias; Duda, Georg N; Tsitsilonis, Serafeim; Keller, Johannes; Maleitzke, Tazio.

In: ARTHRITIS RES THER, Vol. 25, No. 1, 244, 15.12.2023.

Research output: SCORING: Contribution to journalSCORING: Journal articleResearchpeer-review

Harvard

Hildebrandt, A, Dietrich, T, Weber, J, Günderoth, MM, Zhou, S, Fleckenstein, FN, Jiang, S, Winkler, T, Duda, GN, Tsitsilonis, S, Keller, J & Maleitzke, T 2023, 'The dual pro-inflammatory and bone-protective role of calcitonin gene-related peptide alpha in age-related osteoarthritis', ARTHRITIS RES THER, vol. 25, no. 1, 244. https://doi.org/10.1186/s13075-023-03215-3

APA

Hildebrandt, A., Dietrich, T., Weber, J., Günderoth, M. M., Zhou, S., Fleckenstein, F. N., Jiang, S., Winkler, T., Duda, G. N., Tsitsilonis, S., Keller, J., & Maleitzke, T. (2023). The dual pro-inflammatory and bone-protective role of calcitonin gene-related peptide alpha in age-related osteoarthritis. ARTHRITIS RES THER, 25(1), [244]. https://doi.org/10.1186/s13075-023-03215-3

Vancouver

Bibtex

@article{4742f9ce85f842c7aa5ba0c6c2799e6f,
title = "The dual pro-inflammatory and bone-protective role of calcitonin gene-related peptide alpha in age-related osteoarthritis",
abstract = "BACKGROUND: The vasoactive neuropeptide calcitonin gene-related peptide alpha (αCGRP) enhances nociception in primary knee osteoarthritis (OA) and has been shown to disrupt cartilage and joint integrity in experimental rheumatoid arthritis (RA). Little is known about how αCGRP may alter articular structures in primary OA. We investigated whether αCGRP modulates local inflammation and concomitant cartilage and bone changes in a murine model of age-dependent OA.METHODS: Sixteen- to 18-month-old αCGRP-deficient mice (αCGRP-/-aged) were compared to, first, age-matched wild type (WTaged) and, second, young 4- to 5-month-old non-OA αCGRP-deficient (αCGRP-/-CTRL) and non-OA WT animals (WTCTRL). αCGRP levels were measured in serum. Knee and hip joint inflammation, cartilage degradation, and bone alterations were assessed by histology (OARSI histopathological grading score), gene expression analysis, and µ-computed tomography.RESULTS: WTaged mice exhibited elevated αCGRP serum levels compared to young WTCTRL animals. Marked signs of OA-induced cartilage destruction were seen in WTaged animals, while αCGRP-/-aged mice were mostly protected from this effect. Age-dependent OA was accompanied by an increased gene expression of pro-inflammatory Tnfa, Il1b, and Il6 and catabolic Mmp13, Adamts5, Ctsk, Tnfs11 (Rankl), and Cxcl12/Cxcr4 in WTaged but not in αCGRP-/-aged mice. αCGRP-deficiency however further aggravated subchondral bone sclerosis of the medial tibial plateau and accelerated bone loss in the epi- and metaphyseal trabecular tibial bone in age-dependent OA.CONCLUSIONS: Similar to its function in experimental RA, αCGRP exerts a dual pro-inflammatory and bone-protective function in murine primary OA. Although anti-CGRP treatment was previously not successful in reducing pain in OA clinically, these data underline a crucial pathophysiological role of αCGRP in age-related OA.",
author = "Alexander Hildebrandt and Tamara Dietrich and J{\'e}r{\^o}me Weber and G{\"u}nderoth, {Mara Meyer} and Sijia Zhou and Fleckenstein, {Florian N} and Shan Jiang and Tobias Winkler and Duda, {Georg N} and Serafeim Tsitsilonis and Johannes Keller and Tazio Maleitzke",
note = "{\textcopyright} 2023. The Author(s).",
year = "2023",
month = dec,
day = "15",
doi = "10.1186/s13075-023-03215-3",
language = "English",
volume = "25",
journal = "ARTHRITIS RES THER",
issn = "1478-6354",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - The dual pro-inflammatory and bone-protective role of calcitonin gene-related peptide alpha in age-related osteoarthritis

AU - Hildebrandt, Alexander

AU - Dietrich, Tamara

AU - Weber, Jérôme

AU - Günderoth, Mara Meyer

AU - Zhou, Sijia

AU - Fleckenstein, Florian N

AU - Jiang, Shan

AU - Winkler, Tobias

AU - Duda, Georg N

AU - Tsitsilonis, Serafeim

AU - Keller, Johannes

AU - Maleitzke, Tazio

N1 - © 2023. The Author(s).

PY - 2023/12/15

Y1 - 2023/12/15

N2 - BACKGROUND: The vasoactive neuropeptide calcitonin gene-related peptide alpha (αCGRP) enhances nociception in primary knee osteoarthritis (OA) and has been shown to disrupt cartilage and joint integrity in experimental rheumatoid arthritis (RA). Little is known about how αCGRP may alter articular structures in primary OA. We investigated whether αCGRP modulates local inflammation and concomitant cartilage and bone changes in a murine model of age-dependent OA.METHODS: Sixteen- to 18-month-old αCGRP-deficient mice (αCGRP-/-aged) were compared to, first, age-matched wild type (WTaged) and, second, young 4- to 5-month-old non-OA αCGRP-deficient (αCGRP-/-CTRL) and non-OA WT animals (WTCTRL). αCGRP levels were measured in serum. Knee and hip joint inflammation, cartilage degradation, and bone alterations were assessed by histology (OARSI histopathological grading score), gene expression analysis, and µ-computed tomography.RESULTS: WTaged mice exhibited elevated αCGRP serum levels compared to young WTCTRL animals. Marked signs of OA-induced cartilage destruction were seen in WTaged animals, while αCGRP-/-aged mice were mostly protected from this effect. Age-dependent OA was accompanied by an increased gene expression of pro-inflammatory Tnfa, Il1b, and Il6 and catabolic Mmp13, Adamts5, Ctsk, Tnfs11 (Rankl), and Cxcl12/Cxcr4 in WTaged but not in αCGRP-/-aged mice. αCGRP-deficiency however further aggravated subchondral bone sclerosis of the medial tibial plateau and accelerated bone loss in the epi- and metaphyseal trabecular tibial bone in age-dependent OA.CONCLUSIONS: Similar to its function in experimental RA, αCGRP exerts a dual pro-inflammatory and bone-protective function in murine primary OA. Although anti-CGRP treatment was previously not successful in reducing pain in OA clinically, these data underline a crucial pathophysiological role of αCGRP in age-related OA.

AB - BACKGROUND: The vasoactive neuropeptide calcitonin gene-related peptide alpha (αCGRP) enhances nociception in primary knee osteoarthritis (OA) and has been shown to disrupt cartilage and joint integrity in experimental rheumatoid arthritis (RA). Little is known about how αCGRP may alter articular structures in primary OA. We investigated whether αCGRP modulates local inflammation and concomitant cartilage and bone changes in a murine model of age-dependent OA.METHODS: Sixteen- to 18-month-old αCGRP-deficient mice (αCGRP-/-aged) were compared to, first, age-matched wild type (WTaged) and, second, young 4- to 5-month-old non-OA αCGRP-deficient (αCGRP-/-CTRL) and non-OA WT animals (WTCTRL). αCGRP levels were measured in serum. Knee and hip joint inflammation, cartilage degradation, and bone alterations were assessed by histology (OARSI histopathological grading score), gene expression analysis, and µ-computed tomography.RESULTS: WTaged mice exhibited elevated αCGRP serum levels compared to young WTCTRL animals. Marked signs of OA-induced cartilage destruction were seen in WTaged animals, while αCGRP-/-aged mice were mostly protected from this effect. Age-dependent OA was accompanied by an increased gene expression of pro-inflammatory Tnfa, Il1b, and Il6 and catabolic Mmp13, Adamts5, Ctsk, Tnfs11 (Rankl), and Cxcl12/Cxcr4 in WTaged but not in αCGRP-/-aged mice. αCGRP-deficiency however further aggravated subchondral bone sclerosis of the medial tibial plateau and accelerated bone loss in the epi- and metaphyseal trabecular tibial bone in age-dependent OA.CONCLUSIONS: Similar to its function in experimental RA, αCGRP exerts a dual pro-inflammatory and bone-protective function in murine primary OA. Although anti-CGRP treatment was previously not successful in reducing pain in OA clinically, these data underline a crucial pathophysiological role of αCGRP in age-related OA.

U2 - 10.1186/s13075-023-03215-3

DO - 10.1186/s13075-023-03215-3

M3 - SCORING: Journal article

C2 - 38102666

VL - 25

JO - ARTHRITIS RES THER

JF - ARTHRITIS RES THER

SN - 1478-6354

IS - 1

M1 - 244

ER -