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The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney.

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The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney. / Behrends, S; Budäus, Lars; Kempfert, J; Scholz, H; Starbatty, J; Vehse, K.

In: N-S ARCH PHARMACOL, Vol. 364, No. 6, 6, 2001, p. 573-576.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Behrends, S, Budäus, L, Kempfert, J, Scholz, H, Starbatty, J & Vehse, K 2001, 'The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney.', N-S ARCH PHARMACOL, vol. 364, no. 6, 6, pp. 573-576. <http://www.ncbi.nlm.nih.gov/pubmed/11770014?dopt=Citation>

APA

Behrends, S., Budäus, L., Kempfert, J., Scholz, H., Starbatty, J., & Vehse, K. (2001). The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney. N-S ARCH PHARMACOL, 364(6), 573-576. [6]. http://www.ncbi.nlm.nih.gov/pubmed/11770014?dopt=Citation

Vancouver

Behrends S, Budäus L, Kempfert J, Scholz H, Starbatty J, Vehse K. The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney. N-S ARCH PHARMACOL. 2001;364(6):573-576. 6.

Bibtex

@article{6191af71554a48e0b62a4c88c7ffff8f,
title = "The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney.",
abstract = "We have recently shown that nitric oxide activates the beta2 subunit of soluble guanylyl cyclase. In the present study, we show developmental regulation of this subunit. Analysis of mRNA expression by RT-PCR and RNase protection analysis in kidneys revealed no expression of the beta2 subunit in neonatal and strong expression in adult rats. A reciprocal regulation with much lower expression levels was observed in rat lung. Further examination of kidneys from 3, 6, 16, 22, 25, 31 and 36-day-old rats showed that significant expression appears between postnatal day 16 and 22. Isolation of the rat beta2 promoter by genome walking and cloning into a reporter gene vector showed promoter activity for the sense but not the antisense construct providing an in vitro assay for further analysis of the developmental beta2 subunit regulation.",
author = "S Behrends and Lars Bud{\"a}us and J Kempfert and H Scholz and J Starbatty and K Vehse",
year = "2001",
language = "Deutsch",
volume = "364",
pages = "573--576",
journal = "N-S ARCH PHARMACOL",
issn = "0028-1298",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney.

AU - Behrends, S

AU - Budäus, Lars

AU - Kempfert, J

AU - Scholz, H

AU - Starbatty, J

AU - Vehse, K

PY - 2001

Y1 - 2001

N2 - We have recently shown that nitric oxide activates the beta2 subunit of soluble guanylyl cyclase. In the present study, we show developmental regulation of this subunit. Analysis of mRNA expression by RT-PCR and RNase protection analysis in kidneys revealed no expression of the beta2 subunit in neonatal and strong expression in adult rats. A reciprocal regulation with much lower expression levels was observed in rat lung. Further examination of kidneys from 3, 6, 16, 22, 25, 31 and 36-day-old rats showed that significant expression appears between postnatal day 16 and 22. Isolation of the rat beta2 promoter by genome walking and cloning into a reporter gene vector showed promoter activity for the sense but not the antisense construct providing an in vitro assay for further analysis of the developmental beta2 subunit regulation.

AB - We have recently shown that nitric oxide activates the beta2 subunit of soluble guanylyl cyclase. In the present study, we show developmental regulation of this subunit. Analysis of mRNA expression by RT-PCR and RNase protection analysis in kidneys revealed no expression of the beta2 subunit in neonatal and strong expression in adult rats. A reciprocal regulation with much lower expression levels was observed in rat lung. Further examination of kidneys from 3, 6, 16, 22, 25, 31 and 36-day-old rats showed that significant expression appears between postnatal day 16 and 22. Isolation of the rat beta2 promoter by genome walking and cloning into a reporter gene vector showed promoter activity for the sense but not the antisense construct providing an in vitro assay for further analysis of the developmental beta2 subunit regulation.

M3 - SCORING: Zeitschriftenaufsatz

VL - 364

SP - 573

EP - 576

JO - N-S ARCH PHARMACOL

JF - N-S ARCH PHARMACOL

SN - 0028-1298

IS - 6

M1 - 6

ER -