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The added value of a commercial 16S/18S-PCR assay (UMD-SelectNA, Molzym) for microbiological diagnosis of spondylodiscitis:an observational study

Standard

The added value of a commercial 16S/18S-PCR assay (UMD-SelectNA, Molzym) for microbiological diagnosis of spondylodiscitis:an observational study. / Both, Anna; Christner, Martin; Berinson, Benjamin; Dreimann, Marc; Viezens, Lennart; Lütgehetmann, Marc; Aepfelbacher, Martin; Rohde, Holger; Stangenberg, Martin.

In: DIAGN MICR INFEC DIS, Vol. 106, No. 1, 115926, 05.2023.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Both, A, Christner, M, Berinson, B, Dreimann, M, Viezens, L, Lütgehetmann, M, Aepfelbacher, M, Rohde, H & Stangenberg, M 2023, 'The added value of a commercial 16S/18S-PCR assay (UMD-SelectNA, Molzym) for microbiological diagnosis of spondylodiscitis:an observational study', DIAGN MICR INFEC DIS, vol. 106, no. 1, 115926. https://doi.org/10.1016/j.diagmicrobio.2023.115926

APA

Both, A., Christner, M., Berinson, B., Dreimann, M., Viezens, L., Lütgehetmann, M., Aepfelbacher, M., Rohde, H., & Stangenberg, M. (2023). The added value of a commercial 16S/18S-PCR assay (UMD-SelectNA, Molzym) for microbiological diagnosis of spondylodiscitis:an observational study. DIAGN MICR INFEC DIS, 106(1), [115926]. https://doi.org/10.1016/j.diagmicrobio.2023.115926

Vancouver

Both A, Christner M, Berinson B, Dreimann M, Viezens L, Lütgehetmann M et al. The added value of a commercial 16S/18S-PCR assay (UMD-SelectNA, Molzym) for microbiological diagnosis of spondylodiscitis:an observational study. DIAGN MICR INFEC DIS. 2023 May;106(1). 115926. https://doi.org/10.1016/j.diagmicrobio.2023.115926

Bibtex

@article{88a06908235b47e69bf65e99fc1be9b8,
title = "The added value of a commercial 16S/18S-PCR assay (UMD-SelectNA, Molzym) for microbiological diagnosis of spondylodiscitis:an observational study",
abstract = "In spondylodiscitis, pathogen identification is important to guide therapy strategies. Here the use of an rDNA PCR assay (Molzym UMDSelectNA) for pathogen detection in spondylodiscitis was evaluated in 182 specimens from 124 spondylodiscitis patients. In 81% of specimens rDNA PCR and conventional culture produced concordant results. Compared to conventional culture, sensitivity and specificity of rDNA PCR were 75% and 83.9%, respectively. The rDNA PCR performed better than conventional culture in identification of Streptococcus spp.. However, overall sensitivity was suboptimal, e.g., in cases with low bacterial burden, and only 5 of 124 patients (4%) received a microbiological diagnosis by employing rDNA PCR. Thus, the added value of routine use of rDNA PCR on spondylodiscitis specimens is limited. Targeted use of the assay in culture-negative cases may be efficient and moderately increase diagnostic yield. The need for susceptibility information implies that 16S rDNA PCR may only be used as an add-on tool to culture.",
author = "Anna Both and Martin Christner and Benjamin Berinson and Marc Dreimann and Lennart Viezens and Marc L{\"u}tgehetmann and Martin Aepfelbacher and Holger Rohde and Martin Stangenberg",
note = "Copyright {\textcopyright} 2023 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2023",
month = may,
doi = "10.1016/j.diagmicrobio.2023.115926",
language = "English",
volume = "106",
journal = "DIAGN MICR INFEC DIS",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - The added value of a commercial 16S/18S-PCR assay (UMD-SelectNA, Molzym) for microbiological diagnosis of spondylodiscitis:an observational study

AU - Both, Anna

AU - Christner, Martin

AU - Berinson, Benjamin

AU - Dreimann, Marc

AU - Viezens, Lennart

AU - Lütgehetmann, Marc

AU - Aepfelbacher, Martin

AU - Rohde, Holger

AU - Stangenberg, Martin

N1 - Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2023/5

Y1 - 2023/5

N2 - In spondylodiscitis, pathogen identification is important to guide therapy strategies. Here the use of an rDNA PCR assay (Molzym UMDSelectNA) for pathogen detection in spondylodiscitis was evaluated in 182 specimens from 124 spondylodiscitis patients. In 81% of specimens rDNA PCR and conventional culture produced concordant results. Compared to conventional culture, sensitivity and specificity of rDNA PCR were 75% and 83.9%, respectively. The rDNA PCR performed better than conventional culture in identification of Streptococcus spp.. However, overall sensitivity was suboptimal, e.g., in cases with low bacterial burden, and only 5 of 124 patients (4%) received a microbiological diagnosis by employing rDNA PCR. Thus, the added value of routine use of rDNA PCR on spondylodiscitis specimens is limited. Targeted use of the assay in culture-negative cases may be efficient and moderately increase diagnostic yield. The need for susceptibility information implies that 16S rDNA PCR may only be used as an add-on tool to culture.

AB - In spondylodiscitis, pathogen identification is important to guide therapy strategies. Here the use of an rDNA PCR assay (Molzym UMDSelectNA) for pathogen detection in spondylodiscitis was evaluated in 182 specimens from 124 spondylodiscitis patients. In 81% of specimens rDNA PCR and conventional culture produced concordant results. Compared to conventional culture, sensitivity and specificity of rDNA PCR were 75% and 83.9%, respectively. The rDNA PCR performed better than conventional culture in identification of Streptococcus spp.. However, overall sensitivity was suboptimal, e.g., in cases with low bacterial burden, and only 5 of 124 patients (4%) received a microbiological diagnosis by employing rDNA PCR. Thus, the added value of routine use of rDNA PCR on spondylodiscitis specimens is limited. Targeted use of the assay in culture-negative cases may be efficient and moderately increase diagnostic yield. The need for susceptibility information implies that 16S rDNA PCR may only be used as an add-on tool to culture.

U2 - 10.1016/j.diagmicrobio.2023.115926

DO - 10.1016/j.diagmicrobio.2023.115926

M3 - SCORING: Journal article

C2 - 36963329

VL - 106

JO - DIAGN MICR INFEC DIS

JF - DIAGN MICR INFEC DIS

SN - 0732-8893

IS - 1

M1 - 115926

ER -