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T(H)1 to T(H)2 shift of cytokines in peripheral blood of HIV-infected patients is detectable by reverse transcriptase polymerase chain reaction but not by enzyme-linked immunosorbent assay under nonstimulated conditions

Standard

T(H)1 to T(H)2 shift of cytokines in peripheral blood of HIV-infected patients is detectable by reverse transcriptase polymerase chain reaction but not by enzyme-linked immunosorbent assay under nonstimulated conditions. / Altfeld, M; Addo, Marylyn M.; Kreuzer, K A; Rockstroh, J K; Dumoulin, F L; Schliefer, K; Leifeld, L; Sauerbruch, T; Spengler, U.

In: JAIDS-J ACQ IMM DEF, Vol. 23, No. 4, 01.04.2000, p. 287-94.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Altfeld, M, Addo, MM, Kreuzer, KA, Rockstroh, JK, Dumoulin, FL, Schliefer, K, Leifeld, L, Sauerbruch, T & Spengler, U 2000, 'T(H)1 to T(H)2 shift of cytokines in peripheral blood of HIV-infected patients is detectable by reverse transcriptase polymerase chain reaction but not by enzyme-linked immunosorbent assay under nonstimulated conditions', JAIDS-J ACQ IMM DEF, vol. 23, no. 4, pp. 287-94. https://doi.org/10.1097/00126334-200004010-00001

APA

Altfeld, M., Addo, M. M., Kreuzer, K. A., Rockstroh, J. K., Dumoulin, F. L., Schliefer, K., Leifeld, L., Sauerbruch, T., & Spengler, U. (2000). T(H)1 to T(H)2 shift of cytokines in peripheral blood of HIV-infected patients is detectable by reverse transcriptase polymerase chain reaction but not by enzyme-linked immunosorbent assay under nonstimulated conditions. JAIDS-J ACQ IMM DEF, 23(4), 287-94. https://doi.org/10.1097/00126334-200004010-00001

Vancouver

Altfeld M, Addo MM, Kreuzer KA, Rockstroh JK, Dumoulin FL, Schliefer K et al. T(H)1 to T(H)2 shift of cytokines in peripheral blood of HIV-infected patients is detectable by reverse transcriptase polymerase chain reaction but not by enzyme-linked immunosorbent assay under nonstimulated conditions. JAIDS-J ACQ IMM DEF. 2000 Apr 1;23(4):287-94. https://doi.org/10.1097/00126334-200004010-00001

Bibtex

@article{b330b37c635f45eb97c321089749975e,
title = "T(H)1 to T(H)2 shift of cytokines in peripheral blood of HIV-infected patients is detectable by reverse transcriptase polymerase chain reaction but not by enzyme-linked immunosorbent assay under nonstimulated conditions",
abstract = "BACKGROUND: Dysregulation of cytokines has been implicated in the pathogenesis of HIV infection. Therefore, we determined tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), IL-4, IL-10, and interferon-gamma (IFN-gamma) mRNA and serum levels in HIV-infected patients under nonstimulated conditions.MATERIAL AND METHODS: Blood samples of 32 HIV-infected patients and 10 healthy HIV-negative controls were analyzed. Cytokine serum levels were quantified by enzyme-linked immunosorbent assay (ELISA). Cytokine mRNA levels were determined semiquantitatively by competitive reverse transcriptase polymerase chain reaction (RT-PCR) and expressed as ratios relative to those of beta-actin.RESULTS: Competitive RT-PCR was shown to be more sensitive than protein ELISA in analyzing cytokine production. We found a significant correlation between steady-state mRNA ratios and serum protein levels for TNF-alpha. Significantly higher cytokine mRNA ratios were found in those patients with IL-10 and IFN-gamma levels detectable by ELISA. Steady-state mRNA ratios of TNF-alpha, IL-4, and IL-10 were significantly increased in patients with highly replicative HIV-infection. Furthermore, elevated IL-4:IFN-gamma ratios were related to both high viral load and loss of CD4 cells.DISCUSSION: Determination of steady-state mRNA ratios by semiquantitative RT-PCR represents a sensitive method to analyze cytokines in peripheral blood of HIV-infected patients under nonstimulated conditions. The data obtained with this technique provide further evidence for a T(H)1 to T(H)2 cytokine shift with progressive HIV disease.",
keywords = "CD4 Lymphocyte Count, Cytokines/blood, Enzyme-Linked Immunosorbent Assay/methods, HIV Infections/immunology, HIV-1/genetics, Humans, Lymphocyte Activation/immunology, RNA, Messenger/blood, RNA, Viral/blood, Reverse Transcriptase Polymerase Chain Reaction/methods, Th1 Cells/metabolism, Th2 Cells/metabolism",
author = "M Altfeld and Addo, {Marylyn M.} and Kreuzer, {K A} and Rockstroh, {J K} and Dumoulin, {F L} and K Schliefer and L Leifeld and T Sauerbruch and U Spengler",
year = "2000",
month = apr,
day = "1",
doi = "10.1097/00126334-200004010-00001",
language = "English",
volume = "23",
pages = "287--94",
journal = "JAIDS-J ACQ IMM DEF",
issn = "1525-4135",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

RIS

TY - JOUR

T1 - T(H)1 to T(H)2 shift of cytokines in peripheral blood of HIV-infected patients is detectable by reverse transcriptase polymerase chain reaction but not by enzyme-linked immunosorbent assay under nonstimulated conditions

AU - Altfeld, M

AU - Addo, Marylyn M.

AU - Kreuzer, K A

AU - Rockstroh, J K

AU - Dumoulin, F L

AU - Schliefer, K

AU - Leifeld, L

AU - Sauerbruch, T

AU - Spengler, U

PY - 2000/4/1

Y1 - 2000/4/1

N2 - BACKGROUND: Dysregulation of cytokines has been implicated in the pathogenesis of HIV infection. Therefore, we determined tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), IL-4, IL-10, and interferon-gamma (IFN-gamma) mRNA and serum levels in HIV-infected patients under nonstimulated conditions.MATERIAL AND METHODS: Blood samples of 32 HIV-infected patients and 10 healthy HIV-negative controls were analyzed. Cytokine serum levels were quantified by enzyme-linked immunosorbent assay (ELISA). Cytokine mRNA levels were determined semiquantitatively by competitive reverse transcriptase polymerase chain reaction (RT-PCR) and expressed as ratios relative to those of beta-actin.RESULTS: Competitive RT-PCR was shown to be more sensitive than protein ELISA in analyzing cytokine production. We found a significant correlation between steady-state mRNA ratios and serum protein levels for TNF-alpha. Significantly higher cytokine mRNA ratios were found in those patients with IL-10 and IFN-gamma levels detectable by ELISA. Steady-state mRNA ratios of TNF-alpha, IL-4, and IL-10 were significantly increased in patients with highly replicative HIV-infection. Furthermore, elevated IL-4:IFN-gamma ratios were related to both high viral load and loss of CD4 cells.DISCUSSION: Determination of steady-state mRNA ratios by semiquantitative RT-PCR represents a sensitive method to analyze cytokines in peripheral blood of HIV-infected patients under nonstimulated conditions. The data obtained with this technique provide further evidence for a T(H)1 to T(H)2 cytokine shift with progressive HIV disease.

AB - BACKGROUND: Dysregulation of cytokines has been implicated in the pathogenesis of HIV infection. Therefore, we determined tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), IL-4, IL-10, and interferon-gamma (IFN-gamma) mRNA and serum levels in HIV-infected patients under nonstimulated conditions.MATERIAL AND METHODS: Blood samples of 32 HIV-infected patients and 10 healthy HIV-negative controls were analyzed. Cytokine serum levels were quantified by enzyme-linked immunosorbent assay (ELISA). Cytokine mRNA levels were determined semiquantitatively by competitive reverse transcriptase polymerase chain reaction (RT-PCR) and expressed as ratios relative to those of beta-actin.RESULTS: Competitive RT-PCR was shown to be more sensitive than protein ELISA in analyzing cytokine production. We found a significant correlation between steady-state mRNA ratios and serum protein levels for TNF-alpha. Significantly higher cytokine mRNA ratios were found in those patients with IL-10 and IFN-gamma levels detectable by ELISA. Steady-state mRNA ratios of TNF-alpha, IL-4, and IL-10 were significantly increased in patients with highly replicative HIV-infection. Furthermore, elevated IL-4:IFN-gamma ratios were related to both high viral load and loss of CD4 cells.DISCUSSION: Determination of steady-state mRNA ratios by semiquantitative RT-PCR represents a sensitive method to analyze cytokines in peripheral blood of HIV-infected patients under nonstimulated conditions. The data obtained with this technique provide further evidence for a T(H)1 to T(H)2 cytokine shift with progressive HIV disease.

KW - CD4 Lymphocyte Count

KW - Cytokines/blood

KW - Enzyme-Linked Immunosorbent Assay/methods

KW - HIV Infections/immunology

KW - HIV-1/genetics

KW - Humans

KW - Lymphocyte Activation/immunology

KW - RNA, Messenger/blood

KW - RNA, Viral/blood

KW - Reverse Transcriptase Polymerase Chain Reaction/methods

KW - Th1 Cells/metabolism

KW - Th2 Cells/metabolism

U2 - 10.1097/00126334-200004010-00001

DO - 10.1097/00126334-200004010-00001

M3 - SCORING: Journal article

C2 - 10836750

VL - 23

SP - 287

EP - 294

JO - JAIDS-J ACQ IMM DEF

JF - JAIDS-J ACQ IMM DEF

SN - 1525-4135

IS - 4

ER -