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Temporal and spatial dynamics of cerebral immune cell accumulation in stroke.

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Temporal and spatial dynamics of cerebral immune cell accumulation in stroke. / Gelderblom, Mathias; Leypoldt, Frank; Steinbach, Karin; Behrens, Doerthe; Choe, Chi-Un; Siler, Dominic A; Arumugam, Thiruma V; Orthey, Ellen; Gerloff, Christian; Tolosa, Eva; Magnus, Tim.

In: STROKE, Vol. 40, No. 5, 5, 2009, p. 1849-1857.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Gelderblom, M, Leypoldt, F, Steinbach, K, Behrens, D, Choe, C-U, Siler, DA, Arumugam, TV, Orthey, E, Gerloff, C, Tolosa, E & Magnus, T 2009, 'Temporal and spatial dynamics of cerebral immune cell accumulation in stroke.', STROKE, vol. 40, no. 5, 5, pp. 1849-1857. <http://www.ncbi.nlm.nih.gov/pubmed/19265055?dopt=Citation>

APA

Gelderblom, M., Leypoldt, F., Steinbach, K., Behrens, D., Choe, C-U., Siler, D. A., Arumugam, T. V., Orthey, E., Gerloff, C., Tolosa, E., & Magnus, T. (2009). Temporal and spatial dynamics of cerebral immune cell accumulation in stroke. STROKE, 40(5), 1849-1857. [5]. http://www.ncbi.nlm.nih.gov/pubmed/19265055?dopt=Citation

Vancouver

Gelderblom M, Leypoldt F, Steinbach K, Behrens D, Choe C-U, Siler DA et al. Temporal and spatial dynamics of cerebral immune cell accumulation in stroke. STROKE. 2009;40(5):1849-1857. 5.

Bibtex

@article{f21113a0918e420481e507c513eed347,
title = "Temporal and spatial dynamics of cerebral immune cell accumulation in stroke.",
abstract = "BACKGROUND AND PURPOSE: Ischemic stroke leads to significant morbidity and mortality in the Western world. Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage. The understanding of postischemic inflammation is very limited. The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia-reperfusion injury model. METHODS: Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice. Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets. RESULTS: Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx. DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells. DCs exhibited a significant upregulation of major histocompatibility complex II and major histocompatibility complex II high-expressing DCs were found 100 times more abundant than in sham conditions. Upregulation of the costimulatory molecule CD80 was observed in DCs and microglial cells but did not further increase in major histocompatibility complex II high-expressing DCs. No lymphocyte activation was observed. Additionally, regulatory immune cells (natural killer T-cells, CD4(-)/CD8(-)T lymphocytes) cumulated in the ischemic hemisphere. CONCLUSIONS: This study provides a detailed analysis of the temporal dynamics of immune cell accumulation in a rodent stroke model. The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.",
author = "Mathias Gelderblom and Frank Leypoldt and Karin Steinbach and Doerthe Behrens and Chi-Un Choe and Siler, {Dominic A} and Arumugam, {Thiruma V} and Ellen Orthey and Christian Gerloff and Eva Tolosa and Tim Magnus",
year = "2009",
language = "Deutsch",
volume = "40",
pages = "1849--1857",
journal = "STROKE",
issn = "0039-2499",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

RIS

TY - JOUR

T1 - Temporal and spatial dynamics of cerebral immune cell accumulation in stroke.

AU - Gelderblom, Mathias

AU - Leypoldt, Frank

AU - Steinbach, Karin

AU - Behrens, Doerthe

AU - Choe, Chi-Un

AU - Siler, Dominic A

AU - Arumugam, Thiruma V

AU - Orthey, Ellen

AU - Gerloff, Christian

AU - Tolosa, Eva

AU - Magnus, Tim

PY - 2009

Y1 - 2009

N2 - BACKGROUND AND PURPOSE: Ischemic stroke leads to significant morbidity and mortality in the Western world. Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage. The understanding of postischemic inflammation is very limited. The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia-reperfusion injury model. METHODS: Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice. Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets. RESULTS: Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx. DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells. DCs exhibited a significant upregulation of major histocompatibility complex II and major histocompatibility complex II high-expressing DCs were found 100 times more abundant than in sham conditions. Upregulation of the costimulatory molecule CD80 was observed in DCs and microglial cells but did not further increase in major histocompatibility complex II high-expressing DCs. No lymphocyte activation was observed. Additionally, regulatory immune cells (natural killer T-cells, CD4(-)/CD8(-)T lymphocytes) cumulated in the ischemic hemisphere. CONCLUSIONS: This study provides a detailed analysis of the temporal dynamics of immune cell accumulation in a rodent stroke model. The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.

AB - BACKGROUND AND PURPOSE: Ischemic stroke leads to significant morbidity and mortality in the Western world. Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage. The understanding of postischemic inflammation is very limited. The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia-reperfusion injury model. METHODS: Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice. Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets. RESULTS: Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx. DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells. DCs exhibited a significant upregulation of major histocompatibility complex II and major histocompatibility complex II high-expressing DCs were found 100 times more abundant than in sham conditions. Upregulation of the costimulatory molecule CD80 was observed in DCs and microglial cells but did not further increase in major histocompatibility complex II high-expressing DCs. No lymphocyte activation was observed. Additionally, regulatory immune cells (natural killer T-cells, CD4(-)/CD8(-)T lymphocytes) cumulated in the ischemic hemisphere. CONCLUSIONS: This study provides a detailed analysis of the temporal dynamics of immune cell accumulation in a rodent stroke model. The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.

M3 - SCORING: Zeitschriftenaufsatz

VL - 40

SP - 1849

EP - 1857

JO - STROKE

JF - STROKE

SN - 0039-2499

IS - 5

M1 - 5

ER -