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Technical Advance: a new cell preparation strategy that greatly improves the yield of vital and functional Tregs and NKT cells

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Technical Advance: a new cell preparation strategy that greatly improves the yield of vital and functional Tregs and NKT cells. / Rissiek, Björn; Danquah, Welbeck; Haag, Friedrich; Nolte, Friedrich.

In: J LEUKOCYTE BIOL, Vol. 95, No. 3, 01.03.2014, p. 543-9.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Rissiek, B, Danquah, W, Haag, F & Nolte, F 2014, 'Technical Advance: a new cell preparation strategy that greatly improves the yield of vital and functional Tregs and NKT cells', J LEUKOCYTE BIOL, vol. 95, no. 3, pp. 543-9. https://doi.org/10.1189/jlb.0713407

APA

Rissiek, B., Danquah, W., Haag, F., & Nolte, F. (2014). Technical Advance: a new cell preparation strategy that greatly improves the yield of vital and functional Tregs and NKT cells. J LEUKOCYTE BIOL, 95(3), 543-9. https://doi.org/10.1189/jlb.0713407

Vancouver

Rissiek B, Danquah W, Haag F, Nolte F. Technical Advance: a new cell preparation strategy that greatly improves the yield of vital and functional Tregs and NKT cells. J LEUKOCYTE BIOL. 2014 Mar 1;95(3):543-9. https://doi.org/10.1189/jlb.0713407

Bibtex

@article{7718648d8de24d83a282199d4439c100,
title = "Technical Advance: a new cell preparation strategy that greatly improves the yield of vital and functional Tregs and NKT cells",
abstract = "Release of NAD(+) during preparation of murine lymphocytes causes enzymatic ADP-ribosylation of cell-surface proteins on T cells, catalyzed by toxin-related ecto-ADP-ribosyltransferase, ARTC2. ADP-riboslyation activates the cytolytic P2X7 ion channel and affects, in particular, the vitality and function of Tregs and NKT cells. Here, we describe a simple method-injection of an ARTC2-blocking nanobody-to greatly improve Treg and NKT cell vitality and to preserve their function during in vitro assays and in adoptive-transfer experiments. Moreover, we present a method for the sorting of functional, primary NKT cells, based on coexpression of ARTC2 and NK1.1. Our results pave the way for the efficient ex vivo proliferation of Tregs and NKT cells and for new experimental and therapeutic uses of these important regulatory cells.",
keywords = "ADP Ribose Transferases, Adoptive Transfer, Animals, Antibodies, Blocking, Cell Separation, Cell Survival, Flow Cytometry, Mice, Mice, Inbred C57BL, Mice, Knockout, Natural Killer T-Cells, Single-Domain Antibodies, T-Lymphocytes, Regulatory",
author = "Bj{\"o}rn Rissiek and Welbeck Danquah and Friedrich Haag and Friedrich Nolte",
year = "2014",
month = mar,
day = "1",
doi = "10.1189/jlb.0713407",
language = "English",
volume = "95",
pages = "543--9",
journal = "J LEUKOCYTE BIOL",
issn = "0741-5400",
publisher = "FASEB",
number = "3",

}

RIS

TY - JOUR

T1 - Technical Advance: a new cell preparation strategy that greatly improves the yield of vital and functional Tregs and NKT cells

AU - Rissiek, Björn

AU - Danquah, Welbeck

AU - Haag, Friedrich

AU - Nolte, Friedrich

PY - 2014/3/1

Y1 - 2014/3/1

N2 - Release of NAD(+) during preparation of murine lymphocytes causes enzymatic ADP-ribosylation of cell-surface proteins on T cells, catalyzed by toxin-related ecto-ADP-ribosyltransferase, ARTC2. ADP-riboslyation activates the cytolytic P2X7 ion channel and affects, in particular, the vitality and function of Tregs and NKT cells. Here, we describe a simple method-injection of an ARTC2-blocking nanobody-to greatly improve Treg and NKT cell vitality and to preserve their function during in vitro assays and in adoptive-transfer experiments. Moreover, we present a method for the sorting of functional, primary NKT cells, based on coexpression of ARTC2 and NK1.1. Our results pave the way for the efficient ex vivo proliferation of Tregs and NKT cells and for new experimental and therapeutic uses of these important regulatory cells.

AB - Release of NAD(+) during preparation of murine lymphocytes causes enzymatic ADP-ribosylation of cell-surface proteins on T cells, catalyzed by toxin-related ecto-ADP-ribosyltransferase, ARTC2. ADP-riboslyation activates the cytolytic P2X7 ion channel and affects, in particular, the vitality and function of Tregs and NKT cells. Here, we describe a simple method-injection of an ARTC2-blocking nanobody-to greatly improve Treg and NKT cell vitality and to preserve their function during in vitro assays and in adoptive-transfer experiments. Moreover, we present a method for the sorting of functional, primary NKT cells, based on coexpression of ARTC2 and NK1.1. Our results pave the way for the efficient ex vivo proliferation of Tregs and NKT cells and for new experimental and therapeutic uses of these important regulatory cells.

KW - ADP Ribose Transferases

KW - Adoptive Transfer

KW - Animals

KW - Antibodies, Blocking

KW - Cell Separation

KW - Cell Survival

KW - Flow Cytometry

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Natural Killer T-Cells

KW - Single-Domain Antibodies

KW - T-Lymphocytes, Regulatory

U2 - 10.1189/jlb.0713407

DO - 10.1189/jlb.0713407

M3 - SCORING: Journal article

C2 - 24212099

VL - 95

SP - 543

EP - 549

JO - J LEUKOCYTE BIOL

JF - J LEUKOCYTE BIOL

SN - 0741-5400

IS - 3

ER -