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TCR repertoire analysis reveals phosphoantigen-induced polyclonal proliferation of Vγ9Vδ2 T cells in neonates and adults

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TCR repertoire analysis reveals phosphoantigen-induced polyclonal proliferation of Vγ9Vδ2 T cells in neonates and adults. / Fichtner, Alina S; Bubke, Anja; Rampoldi, Francesca; Tan, Likai; Wilharm, Anneke; Steinbrück, Lars; Schultze-Florey, Christian; von Kaisenberg, Constantin; Prinz, Immo; Herrmann, Thomas; Ravens, Sarina.

In: J LEUKOCYTE BIOL, Vol. 107, No. 6, 06.2020, p. 1023-1032.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Fichtner, AS, Bubke, A, Rampoldi, F, Tan, L, Wilharm, A, Steinbrück, L, Schultze-Florey, C, von Kaisenberg, C, Prinz, I, Herrmann, T & Ravens, S 2020, 'TCR repertoire analysis reveals phosphoantigen-induced polyclonal proliferation of Vγ9Vδ2 T cells in neonates and adults', J LEUKOCYTE BIOL, vol. 107, no. 6, pp. 1023-1032. https://doi.org/10.1002/JLB.1MA0120-427RR

APA

Fichtner, A. S., Bubke, A., Rampoldi, F., Tan, L., Wilharm, A., Steinbrück, L., Schultze-Florey, C., von Kaisenberg, C., Prinz, I., Herrmann, T., & Ravens, S. (2020). TCR repertoire analysis reveals phosphoantigen-induced polyclonal proliferation of Vγ9Vδ2 T cells in neonates and adults. J LEUKOCYTE BIOL, 107(6), 1023-1032. https://doi.org/10.1002/JLB.1MA0120-427RR

Vancouver

Fichtner AS, Bubke A, Rampoldi F, Tan L, Wilharm A, Steinbrück L et al. TCR repertoire analysis reveals phosphoantigen-induced polyclonal proliferation of Vγ9Vδ2 T cells in neonates and adults. J LEUKOCYTE BIOL. 2020 Jun;107(6):1023-1032. https://doi.org/10.1002/JLB.1MA0120-427RR

Bibtex

@article{b14ec6287d504897ad265cc3d999cf68,
title = "TCR repertoire analysis reveals phosphoantigen-induced polyclonal proliferation of Vγ9Vδ2 T cells in neonates and adults",
abstract = "The Vγ9Vδ2 T cell subset is the major γδ T cell subset in human peripheral blood and has the unique ability to contribute to immune surveillance by detecting pyrophosphorylated metabolites of isoprenoid synthesis, termed phosphoantigens (pAgs). Vγ9Vδ2 T cells are first detected at midgestation and show postnatal expansion. Interestingly, neonatal Vγ9Vδ2 T cells display a higher TCR repertoire diversity with more public clonotypes and lower pAg responsiveness than in adults. Notably, it is not known whether postnatal changes occur by TCR-dependent reactivity to pAg exposure. Here, we applied next-generation sequencing of γδ TCR repertoires to understand potential differences in the pAg-mediated response of neonatal and adult Vγ9Vδ2 T cells at the level of the expressed γδ TCR. We observed a polyclonal pAg-induced response of neonatal and adult Vγ9Vδ2 T cells, albeit neonatal γδ T cells showed less in vitro pAg responsiveness. Neonatal Vγ9Vδ2 T cells displayed a less pronounced bias for Jδ1 usage and a more frequent use of Jδ2 or Jδ3 that remained stable after pAg exposure. In addition, public and private Vδ2 TRD clones took part in the polyclonal pAg-induced response in neonates and adults. In conclusion, adult and neonatal Vγ9Vδ2 T cells both undergo polyclonal pAg-induced proliferation, whereas especially adult Vγ9Vδ2 T cells display a high stability at the level of the expressed TCR repertoire.",
keywords = "Adult, Cell Proliferation/drug effects, Clone Cells, Fetal Blood/cytology, Gene Expression, High-Throughput Nucleotide Sequencing, Humans, Infant, Newborn, Interleukin-2/pharmacology, Lymphocyte Activation/drug effects, Organophosphates/pharmacology, Phosphoproteins/genetics, Primary Cell Culture, Receptors, Antigen, T-Cell, gamma-delta/genetics, T-Lymphocyte Subsets/cytology, Zoledronic Acid/pharmacology",
author = "Fichtner, {Alina S} and Anja Bubke and Francesca Rampoldi and Likai Tan and Anneke Wilharm and Lars Steinbr{\"u}ck and Christian Schultze-Florey and {von Kaisenberg}, Constantin and Immo Prinz and Thomas Herrmann and Sarina Ravens",
note = "{\textcopyright}2020 Society for Leukocyte Biology.",
year = "2020",
month = jun,
doi = "10.1002/JLB.1MA0120-427RR",
language = "English",
volume = "107",
pages = "1023--1032",
journal = "J LEUKOCYTE BIOL",
issn = "0741-5400",
publisher = "FASEB",
number = "6",

}

RIS

TY - JOUR

T1 - TCR repertoire analysis reveals phosphoantigen-induced polyclonal proliferation of Vγ9Vδ2 T cells in neonates and adults

AU - Fichtner, Alina S

AU - Bubke, Anja

AU - Rampoldi, Francesca

AU - Tan, Likai

AU - Wilharm, Anneke

AU - Steinbrück, Lars

AU - Schultze-Florey, Christian

AU - von Kaisenberg, Constantin

AU - Prinz, Immo

AU - Herrmann, Thomas

AU - Ravens, Sarina

N1 - ©2020 Society for Leukocyte Biology.

PY - 2020/6

Y1 - 2020/6

N2 - The Vγ9Vδ2 T cell subset is the major γδ T cell subset in human peripheral blood and has the unique ability to contribute to immune surveillance by detecting pyrophosphorylated metabolites of isoprenoid synthesis, termed phosphoantigens (pAgs). Vγ9Vδ2 T cells are first detected at midgestation and show postnatal expansion. Interestingly, neonatal Vγ9Vδ2 T cells display a higher TCR repertoire diversity with more public clonotypes and lower pAg responsiveness than in adults. Notably, it is not known whether postnatal changes occur by TCR-dependent reactivity to pAg exposure. Here, we applied next-generation sequencing of γδ TCR repertoires to understand potential differences in the pAg-mediated response of neonatal and adult Vγ9Vδ2 T cells at the level of the expressed γδ TCR. We observed a polyclonal pAg-induced response of neonatal and adult Vγ9Vδ2 T cells, albeit neonatal γδ T cells showed less in vitro pAg responsiveness. Neonatal Vγ9Vδ2 T cells displayed a less pronounced bias for Jδ1 usage and a more frequent use of Jδ2 or Jδ3 that remained stable after pAg exposure. In addition, public and private Vδ2 TRD clones took part in the polyclonal pAg-induced response in neonates and adults. In conclusion, adult and neonatal Vγ9Vδ2 T cells both undergo polyclonal pAg-induced proliferation, whereas especially adult Vγ9Vδ2 T cells display a high stability at the level of the expressed TCR repertoire.

AB - The Vγ9Vδ2 T cell subset is the major γδ T cell subset in human peripheral blood and has the unique ability to contribute to immune surveillance by detecting pyrophosphorylated metabolites of isoprenoid synthesis, termed phosphoantigens (pAgs). Vγ9Vδ2 T cells are first detected at midgestation and show postnatal expansion. Interestingly, neonatal Vγ9Vδ2 T cells display a higher TCR repertoire diversity with more public clonotypes and lower pAg responsiveness than in adults. Notably, it is not known whether postnatal changes occur by TCR-dependent reactivity to pAg exposure. Here, we applied next-generation sequencing of γδ TCR repertoires to understand potential differences in the pAg-mediated response of neonatal and adult Vγ9Vδ2 T cells at the level of the expressed γδ TCR. We observed a polyclonal pAg-induced response of neonatal and adult Vγ9Vδ2 T cells, albeit neonatal γδ T cells showed less in vitro pAg responsiveness. Neonatal Vγ9Vδ2 T cells displayed a less pronounced bias for Jδ1 usage and a more frequent use of Jδ2 or Jδ3 that remained stable after pAg exposure. In addition, public and private Vδ2 TRD clones took part in the polyclonal pAg-induced response in neonates and adults. In conclusion, adult and neonatal Vγ9Vδ2 T cells both undergo polyclonal pAg-induced proliferation, whereas especially adult Vγ9Vδ2 T cells display a high stability at the level of the expressed TCR repertoire.

KW - Adult

KW - Cell Proliferation/drug effects

KW - Clone Cells

KW - Fetal Blood/cytology

KW - Gene Expression

KW - High-Throughput Nucleotide Sequencing

KW - Humans

KW - Infant, Newborn

KW - Interleukin-2/pharmacology

KW - Lymphocyte Activation/drug effects

KW - Organophosphates/pharmacology

KW - Phosphoproteins/genetics

KW - Primary Cell Culture

KW - Receptors, Antigen, T-Cell, gamma-delta/genetics

KW - T-Lymphocyte Subsets/cytology

KW - Zoledronic Acid/pharmacology

U2 - 10.1002/JLB.1MA0120-427RR

DO - 10.1002/JLB.1MA0120-427RR

M3 - SCORING: Journal article

C2 - 32064671

VL - 107

SP - 1023

EP - 1032

JO - J LEUKOCYTE BIOL

JF - J LEUKOCYTE BIOL

SN - 0741-5400

IS - 6

ER -