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T-Bet Enhances Regulatory T Cell Fitness and Directs Control of Th1 Responses in Crescentic GN

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T-Bet Enhances Regulatory T Cell Fitness and Directs Control of Th1 Responses in Crescentic GN. / Nosko, Anna; Kluger, Malte A; Diefenhardt, Paul; Melderis, Simon; Wegscheid, Claudia; Tiegs, Gisa; Stahl, Rolf A K; Panzer, Ulf; Steinmetz, Oliver M.

In: J AM SOC NEPHROL, Vol. 28, No. 1, 01.2017, p. 185-196.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Nosko, A, Kluger, MA, Diefenhardt, P, Melderis, S, Wegscheid, C, Tiegs, G, Stahl, RAK, Panzer, U & Steinmetz, OM 2017, 'T-Bet Enhances Regulatory T Cell Fitness and Directs Control of Th1 Responses in Crescentic GN', J AM SOC NEPHROL, vol. 28, no. 1, pp. 185-196. https://doi.org/10.1681/ASN.2015070820

APA

Nosko, A., Kluger, M. A., Diefenhardt, P., Melderis, S., Wegscheid, C., Tiegs, G., Stahl, R. A. K., Panzer, U., & Steinmetz, O. M. (2017). T-Bet Enhances Regulatory T Cell Fitness and Directs Control of Th1 Responses in Crescentic GN. J AM SOC NEPHROL, 28(1), 185-196. https://doi.org/10.1681/ASN.2015070820

Vancouver

Nosko A, Kluger MA, Diefenhardt P, Melderis S, Wegscheid C, Tiegs G et al. T-Bet Enhances Regulatory T Cell Fitness and Directs Control of Th1 Responses in Crescentic GN. J AM SOC NEPHROL. 2017 Jan;28(1):185-196. https://doi.org/10.1681/ASN.2015070820

Bibtex

@article{eacc4091db594ce6a0786e7e813c11d4,
title = "T-Bet Enhances Regulatory T Cell Fitness and Directs Control of Th1 Responses in Crescentic GN",
abstract = "Th1 cells are central pathogenic mediators of crescentic GN (cGN). Mechanisms responsible for Th1 cell downregulation, however, remain widely unknown. Recently, it was proposed that activation of the Th1-characteristic transcription factor T-bet optimizes Foxp3(+) regulatory T (Treg) cells to counteract Th1-type inflammation. Because very little is known about the role of T-bet(+) Treg1 cells in inflammatory diseases, we studied the function of these cells in the nephrotoxic nephritis (NTN) model of cGN. The percentage of Treg1 cells progressively increased in kidneys of nephritic wild-type mice during the course of NTN, indicating their functional importance. Notably, na{\"i}ve Foxp3(Cre)xT-bet(fl/fl) mice, lacking Treg1 cells, showed spontaneous skewing toward Th1 immunity. Furthermore, absence of Treg1 cells resulted in aggravated NTN with selectively dysregulated renal and systemic Th1 responses. Detailed analyses of Treg cells from Foxp3(Cre)xT-bet(fl/fl) mice revealed unaltered cytokine production and suppressive capacity. However, in competitive cotransfer experiments, wild-type Treg cells outcompeted T-bet-deficient Treg cells in terms of population expansion and expression levels of Foxp3, indicating that T-bet expression is crucial for general Treg fitness. Additionally, T-bet-deficient Treg cells lacked expression of the Th1-characteristic trafficking receptor CXCR3, which correlated with significant impairment of renal Treg infiltration. In summary, our data indicate a new subtype of Treg cells in cGN. These Treg1 cells are characterized by activation of the transcription factor T-bet, which enhances the overall fitness of these cells and optimizes their capacity to downregulate Th1 responses by inducing chemokine receptor CXCR3 expression.",
author = "Anna Nosko and Kluger, {Malte A} and Paul Diefenhardt and Simon Melderis and Claudia Wegscheid and Gisa Tiegs and Stahl, {Rolf A K} and Ulf Panzer and Steinmetz, {Oliver M}",
note = "Copyright {\textcopyright} 2016 by the American Society of Nephrology.",
year = "2017",
month = jan,
doi = "10.1681/ASN.2015070820",
language = "English",
volume = "28",
pages = "185--196",
journal = "J AM SOC NEPHROL",
issn = "1046-6673",
publisher = "American Society of Nephrology",
number = "1",

}

RIS

TY - JOUR

T1 - T-Bet Enhances Regulatory T Cell Fitness and Directs Control of Th1 Responses in Crescentic GN

AU - Nosko, Anna

AU - Kluger, Malte A

AU - Diefenhardt, Paul

AU - Melderis, Simon

AU - Wegscheid, Claudia

AU - Tiegs, Gisa

AU - Stahl, Rolf A K

AU - Panzer, Ulf

AU - Steinmetz, Oliver M

N1 - Copyright © 2016 by the American Society of Nephrology.

PY - 2017/1

Y1 - 2017/1

N2 - Th1 cells are central pathogenic mediators of crescentic GN (cGN). Mechanisms responsible for Th1 cell downregulation, however, remain widely unknown. Recently, it was proposed that activation of the Th1-characteristic transcription factor T-bet optimizes Foxp3(+) regulatory T (Treg) cells to counteract Th1-type inflammation. Because very little is known about the role of T-bet(+) Treg1 cells in inflammatory diseases, we studied the function of these cells in the nephrotoxic nephritis (NTN) model of cGN. The percentage of Treg1 cells progressively increased in kidneys of nephritic wild-type mice during the course of NTN, indicating their functional importance. Notably, naïve Foxp3(Cre)xT-bet(fl/fl) mice, lacking Treg1 cells, showed spontaneous skewing toward Th1 immunity. Furthermore, absence of Treg1 cells resulted in aggravated NTN with selectively dysregulated renal and systemic Th1 responses. Detailed analyses of Treg cells from Foxp3(Cre)xT-bet(fl/fl) mice revealed unaltered cytokine production and suppressive capacity. However, in competitive cotransfer experiments, wild-type Treg cells outcompeted T-bet-deficient Treg cells in terms of population expansion and expression levels of Foxp3, indicating that T-bet expression is crucial for general Treg fitness. Additionally, T-bet-deficient Treg cells lacked expression of the Th1-characteristic trafficking receptor CXCR3, which correlated with significant impairment of renal Treg infiltration. In summary, our data indicate a new subtype of Treg cells in cGN. These Treg1 cells are characterized by activation of the transcription factor T-bet, which enhances the overall fitness of these cells and optimizes their capacity to downregulate Th1 responses by inducing chemokine receptor CXCR3 expression.

AB - Th1 cells are central pathogenic mediators of crescentic GN (cGN). Mechanisms responsible for Th1 cell downregulation, however, remain widely unknown. Recently, it was proposed that activation of the Th1-characteristic transcription factor T-bet optimizes Foxp3(+) regulatory T (Treg) cells to counteract Th1-type inflammation. Because very little is known about the role of T-bet(+) Treg1 cells in inflammatory diseases, we studied the function of these cells in the nephrotoxic nephritis (NTN) model of cGN. The percentage of Treg1 cells progressively increased in kidneys of nephritic wild-type mice during the course of NTN, indicating their functional importance. Notably, naïve Foxp3(Cre)xT-bet(fl/fl) mice, lacking Treg1 cells, showed spontaneous skewing toward Th1 immunity. Furthermore, absence of Treg1 cells resulted in aggravated NTN with selectively dysregulated renal and systemic Th1 responses. Detailed analyses of Treg cells from Foxp3(Cre)xT-bet(fl/fl) mice revealed unaltered cytokine production and suppressive capacity. However, in competitive cotransfer experiments, wild-type Treg cells outcompeted T-bet-deficient Treg cells in terms of population expansion and expression levels of Foxp3, indicating that T-bet expression is crucial for general Treg fitness. Additionally, T-bet-deficient Treg cells lacked expression of the Th1-characteristic trafficking receptor CXCR3, which correlated with significant impairment of renal Treg infiltration. In summary, our data indicate a new subtype of Treg cells in cGN. These Treg1 cells are characterized by activation of the transcription factor T-bet, which enhances the overall fitness of these cells and optimizes their capacity to downregulate Th1 responses by inducing chemokine receptor CXCR3 expression.

U2 - 10.1681/ASN.2015070820

DO - 10.1681/ASN.2015070820

M3 - SCORING: Journal article

C2 - 27297951

VL - 28

SP - 185

EP - 196

JO - J AM SOC NEPHROL

JF - J AM SOC NEPHROL

SN - 1046-6673

IS - 1

ER -