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T-bet and Eomes instruct the development of two distinct natural killer cell lineages in the liver and in the bone marrow

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T-bet and Eomes instruct the development of two distinct natural killer cell lineages in the liver and in the bone marrow. / Daussy, Cécile; Faure, Fabrice; Mayol, Katia; Viel, Sébastien; Gasteiger, Georg; Charrier, Emily; Bienvenu, Jacques; Henry, Thomas; Debien, Emilie; Hasan, Uzma A; Marvel, Jacqueline; Yoh, Keigyou; Takahashi, Satoru; Prinz, Immo; de Bernard, Simon; Buffat, Laurent; Walzer, Thierry.

In: J EXP MED, Vol. 211, No. 3, 10.03.2014, p. 563-77.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Daussy, C, Faure, F, Mayol, K, Viel, S, Gasteiger, G, Charrier, E, Bienvenu, J, Henry, T, Debien, E, Hasan, UA, Marvel, J, Yoh, K, Takahashi, S, Prinz, I, de Bernard, S, Buffat, L & Walzer, T 2014, 'T-bet and Eomes instruct the development of two distinct natural killer cell lineages in the liver and in the bone marrow', J EXP MED, vol. 211, no. 3, pp. 563-77. https://doi.org/10.1084/jem.20131560

APA

Daussy, C., Faure, F., Mayol, K., Viel, S., Gasteiger, G., Charrier, E., Bienvenu, J., Henry, T., Debien, E., Hasan, U. A., Marvel, J., Yoh, K., Takahashi, S., Prinz, I., de Bernard, S., Buffat, L., & Walzer, T. (2014). T-bet and Eomes instruct the development of two distinct natural killer cell lineages in the liver and in the bone marrow. J EXP MED, 211(3), 563-77. https://doi.org/10.1084/jem.20131560

Vancouver

Daussy C, Faure F, Mayol K, Viel S, Gasteiger G, Charrier E et al. T-bet and Eomes instruct the development of two distinct natural killer cell lineages in the liver and in the bone marrow. J EXP MED. 2014 Mar 10;211(3):563-77. https://doi.org/10.1084/jem.20131560

Bibtex

@article{2e79fff64cac496cb518944b7d95fcb0,
title = "T-bet and Eomes instruct the development of two distinct natural killer cell lineages in the liver and in the bone marrow",
abstract = "Trail(+)DX5(-)Eomes(-) natural killer (NK) cells arise in the mouse fetal liver and persist in the adult liver. Their relationships with Trail(-)DX5(+) NK cells remain controversial. We generated a novel Eomes-GFP reporter murine model to address this question. We found that Eomes(-) NK cells are not precursors of classical Eomes(+) NK cells but rather constitute a distinct lineage of innate lymphoid cells. Eomes(-) NK cells are strictly dependent on both T-bet and IL-15, similarly to NKT cells. We observed that, in the liver, expression of T-bet in progenitors represses Eomes expression and the development of Eomes(+) NK cells. Reciprocally, the bone marrow (BM) microenvironment restricts T-bet expression in developing NK cells. Ectopic expression of T-bet forces the development of Eomes(-) NK cells, demonstrating that repression of T-bet is essential for the development of Eomes(+) NK cells. Gene profile analyses show that Eomes(-) NK cells share part of their transcriptional program with NKT cells, including genes involved in liver homing and NK cell receptors. Moreover, Eomes(-) NK cells produce a broad range of cytokines, including IL-2 and TNF in vitro and in vivo, during immune responses against vaccinia virus. Thus, mutually exclusive expression of T-bet and Eomes drives the development of different NK cell lineages with complementary functions.",
keywords = "Adoptive Transfer, Animals, Bone Marrow/metabolism, Cell Differentiation/immunology, Cell Lineage/immunology, DNA Primers/genetics, Flow Cytometry, Gene Knock-In Techniques, Killer Cells, Natural/cytology, Liver/metabolism, Mice, Microarray Analysis, Models, Animal, Real-Time Polymerase Chain Reaction, Stem Cell Niche/immunology, T-Box Domain Proteins/genetics",
author = "C{\'e}cile Daussy and Fabrice Faure and Katia Mayol and S{\'e}bastien Viel and Georg Gasteiger and Emily Charrier and Jacques Bienvenu and Thomas Henry and Emilie Debien and Hasan, {Uzma A} and Jacqueline Marvel and Keigyou Yoh and Satoru Takahashi and Immo Prinz and {de Bernard}, Simon and Laurent Buffat and Thierry Walzer",
year = "2014",
month = mar,
day = "10",
doi = "10.1084/jem.20131560",
language = "English",
volume = "211",
pages = "563--77",
journal = "J EXP MED",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "3",

}

RIS

TY - JOUR

T1 - T-bet and Eomes instruct the development of two distinct natural killer cell lineages in the liver and in the bone marrow

AU - Daussy, Cécile

AU - Faure, Fabrice

AU - Mayol, Katia

AU - Viel, Sébastien

AU - Gasteiger, Georg

AU - Charrier, Emily

AU - Bienvenu, Jacques

AU - Henry, Thomas

AU - Debien, Emilie

AU - Hasan, Uzma A

AU - Marvel, Jacqueline

AU - Yoh, Keigyou

AU - Takahashi, Satoru

AU - Prinz, Immo

AU - de Bernard, Simon

AU - Buffat, Laurent

AU - Walzer, Thierry

PY - 2014/3/10

Y1 - 2014/3/10

N2 - Trail(+)DX5(-)Eomes(-) natural killer (NK) cells arise in the mouse fetal liver and persist in the adult liver. Their relationships with Trail(-)DX5(+) NK cells remain controversial. We generated a novel Eomes-GFP reporter murine model to address this question. We found that Eomes(-) NK cells are not precursors of classical Eomes(+) NK cells but rather constitute a distinct lineage of innate lymphoid cells. Eomes(-) NK cells are strictly dependent on both T-bet and IL-15, similarly to NKT cells. We observed that, in the liver, expression of T-bet in progenitors represses Eomes expression and the development of Eomes(+) NK cells. Reciprocally, the bone marrow (BM) microenvironment restricts T-bet expression in developing NK cells. Ectopic expression of T-bet forces the development of Eomes(-) NK cells, demonstrating that repression of T-bet is essential for the development of Eomes(+) NK cells. Gene profile analyses show that Eomes(-) NK cells share part of their transcriptional program with NKT cells, including genes involved in liver homing and NK cell receptors. Moreover, Eomes(-) NK cells produce a broad range of cytokines, including IL-2 and TNF in vitro and in vivo, during immune responses against vaccinia virus. Thus, mutually exclusive expression of T-bet and Eomes drives the development of different NK cell lineages with complementary functions.

AB - Trail(+)DX5(-)Eomes(-) natural killer (NK) cells arise in the mouse fetal liver and persist in the adult liver. Their relationships with Trail(-)DX5(+) NK cells remain controversial. We generated a novel Eomes-GFP reporter murine model to address this question. We found that Eomes(-) NK cells are not precursors of classical Eomes(+) NK cells but rather constitute a distinct lineage of innate lymphoid cells. Eomes(-) NK cells are strictly dependent on both T-bet and IL-15, similarly to NKT cells. We observed that, in the liver, expression of T-bet in progenitors represses Eomes expression and the development of Eomes(+) NK cells. Reciprocally, the bone marrow (BM) microenvironment restricts T-bet expression in developing NK cells. Ectopic expression of T-bet forces the development of Eomes(-) NK cells, demonstrating that repression of T-bet is essential for the development of Eomes(+) NK cells. Gene profile analyses show that Eomes(-) NK cells share part of their transcriptional program with NKT cells, including genes involved in liver homing and NK cell receptors. Moreover, Eomes(-) NK cells produce a broad range of cytokines, including IL-2 and TNF in vitro and in vivo, during immune responses against vaccinia virus. Thus, mutually exclusive expression of T-bet and Eomes drives the development of different NK cell lineages with complementary functions.

KW - Adoptive Transfer

KW - Animals

KW - Bone Marrow/metabolism

KW - Cell Differentiation/immunology

KW - Cell Lineage/immunology

KW - DNA Primers/genetics

KW - Flow Cytometry

KW - Gene Knock-In Techniques

KW - Killer Cells, Natural/cytology

KW - Liver/metabolism

KW - Mice

KW - Microarray Analysis

KW - Models, Animal

KW - Real-Time Polymerase Chain Reaction

KW - Stem Cell Niche/immunology

KW - T-Box Domain Proteins/genetics

U2 - 10.1084/jem.20131560

DO - 10.1084/jem.20131560

M3 - SCORING: Journal article

C2 - 24516120

VL - 211

SP - 563

EP - 577

JO - J EXP MED

JF - J EXP MED

SN - 0022-1007

IS - 3

ER -