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Targeting the lateral but not the third ventricle induces bone loss in ewe: An experimental approach to generate an improved large animal model of osteoporosis.

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Targeting the lateral but not the third ventricle induces bone loss in ewe: An experimental approach to generate an improved large animal model of osteoporosis. / Oheim, Ralf; Beil, Frank Timo; Barvencik, Florian; Egermann, Marcus; Amling, Michael; Clarke, Iain J; Pogoda, Pia.

In: J TRAUMA, Vol. 72, No. 3, 3, 2012, p. 720-726.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Oheim, R, Beil, FT, Barvencik, F, Egermann, M, Amling, M, Clarke, IJ & Pogoda, P 2012, 'Targeting the lateral but not the third ventricle induces bone loss in ewe: An experimental approach to generate an improved large animal model of osteoporosis.', J TRAUMA, vol. 72, no. 3, 3, pp. 720-726. <http://www.ncbi.nlm.nih.gov/pubmed/22491560?dopt=Citation>

APA

Oheim, R., Beil, F. T., Barvencik, F., Egermann, M., Amling, M., Clarke, I. J., & Pogoda, P. (2012). Targeting the lateral but not the third ventricle induces bone loss in ewe: An experimental approach to generate an improved large animal model of osteoporosis. J TRAUMA, 72(3), 720-726. [3]. http://www.ncbi.nlm.nih.gov/pubmed/22491560?dopt=Citation

Vancouver

Oheim R, Beil FT, Barvencik F, Egermann M, Amling M, Clarke IJ et al. Targeting the lateral but not the third ventricle induces bone loss in ewe: An experimental approach to generate an improved large animal model of osteoporosis. J TRAUMA. 2012;72(3):720-726. 3.

Bibtex

@article{0bd0cc1cbb094b7999b0bd2a00bef53a,
title = "Targeting the lateral but not the third ventricle induces bone loss in ewe: An experimental approach to generate an improved large animal model of osteoporosis.",
abstract = "BACKGROUND:: Osteoporosis is a chronic disease characterized by bone loss and increased skeletal fragility. Large animal models are required for preclinical testing of new therapeutic approaches. We have recently demonstrated that continuous intracerebroventricular (ICV) application of leptin into the lateral ventricle (LV) induces bone loss in ewe. On the basis of these findings, we reasoned that the third ventricle (TV) is an even better target because of its closer location to the hypothalamus that mediates leptin effects on bone. METHODS:: Corriedale sheep were randomly mixed to four groups of four ewe each: control entire (control), ovarectomy plus ICV application of cerebrospinal fluid (OVX), OVX plus ICV application of leptin into the LV (leptin-LV); and ICV application of leptin into the TV (leptin-TV). After 3 months, histomorphometric characterization and bone turnover parameters were analyzed. RESULTS:: Highly significant loss of trabecular bone was observed only in leptin-LV group. Increased osteoclast indices and urinary cross-lap excretion were observed in OVX and leptin-TV group. In contrast, serum parameters of osteoblast activity were only significantly decreased in leptin-LV group. Autopsy of ewe brain showed fibrosis around the stainless steel cannula in leptin-TV group. CONCLUSIONS:: ICV application of leptin into the LV strongly reduces bone formation and leads to a highly significant trabecular bone loss in ewe. In contrast, ICV application of leptin into the TV is technically more demanding and results are unpredictable, because the required use of stainless steel cannula induces peri-implant fibrosis that might prevent leptin to enter the cerebrospinal fluid.",
author = "Ralf Oheim and Beil, {Frank Timo} and Florian Barvencik and Marcus Egermann and Michael Amling and Clarke, {Iain J} and Pia Pogoda",
year = "2012",
language = "English",
volume = "72",
pages = "720--726",
number = "3",

}

RIS

TY - JOUR

T1 - Targeting the lateral but not the third ventricle induces bone loss in ewe: An experimental approach to generate an improved large animal model of osteoporosis.

AU - Oheim, Ralf

AU - Beil, Frank Timo

AU - Barvencik, Florian

AU - Egermann, Marcus

AU - Amling, Michael

AU - Clarke, Iain J

AU - Pogoda, Pia

PY - 2012

Y1 - 2012

N2 - BACKGROUND:: Osteoporosis is a chronic disease characterized by bone loss and increased skeletal fragility. Large animal models are required for preclinical testing of new therapeutic approaches. We have recently demonstrated that continuous intracerebroventricular (ICV) application of leptin into the lateral ventricle (LV) induces bone loss in ewe. On the basis of these findings, we reasoned that the third ventricle (TV) is an even better target because of its closer location to the hypothalamus that mediates leptin effects on bone. METHODS:: Corriedale sheep were randomly mixed to four groups of four ewe each: control entire (control), ovarectomy plus ICV application of cerebrospinal fluid (OVX), OVX plus ICV application of leptin into the LV (leptin-LV); and ICV application of leptin into the TV (leptin-TV). After 3 months, histomorphometric characterization and bone turnover parameters were analyzed. RESULTS:: Highly significant loss of trabecular bone was observed only in leptin-LV group. Increased osteoclast indices and urinary cross-lap excretion were observed in OVX and leptin-TV group. In contrast, serum parameters of osteoblast activity were only significantly decreased in leptin-LV group. Autopsy of ewe brain showed fibrosis around the stainless steel cannula in leptin-TV group. CONCLUSIONS:: ICV application of leptin into the LV strongly reduces bone formation and leads to a highly significant trabecular bone loss in ewe. In contrast, ICV application of leptin into the TV is technically more demanding and results are unpredictable, because the required use of stainless steel cannula induces peri-implant fibrosis that might prevent leptin to enter the cerebrospinal fluid.

AB - BACKGROUND:: Osteoporosis is a chronic disease characterized by bone loss and increased skeletal fragility. Large animal models are required for preclinical testing of new therapeutic approaches. We have recently demonstrated that continuous intracerebroventricular (ICV) application of leptin into the lateral ventricle (LV) induces bone loss in ewe. On the basis of these findings, we reasoned that the third ventricle (TV) is an even better target because of its closer location to the hypothalamus that mediates leptin effects on bone. METHODS:: Corriedale sheep were randomly mixed to four groups of four ewe each: control entire (control), ovarectomy plus ICV application of cerebrospinal fluid (OVX), OVX plus ICV application of leptin into the LV (leptin-LV); and ICV application of leptin into the TV (leptin-TV). After 3 months, histomorphometric characterization and bone turnover parameters were analyzed. RESULTS:: Highly significant loss of trabecular bone was observed only in leptin-LV group. Increased osteoclast indices and urinary cross-lap excretion were observed in OVX and leptin-TV group. In contrast, serum parameters of osteoblast activity were only significantly decreased in leptin-LV group. Autopsy of ewe brain showed fibrosis around the stainless steel cannula in leptin-TV group. CONCLUSIONS:: ICV application of leptin into the LV strongly reduces bone formation and leads to a highly significant trabecular bone loss in ewe. In contrast, ICV application of leptin into the TV is technically more demanding and results are unpredictable, because the required use of stainless steel cannula induces peri-implant fibrosis that might prevent leptin to enter the cerebrospinal fluid.

M3 - SCORING: Journal article

VL - 72

SP - 720

EP - 726

IS - 3

M1 - 3

ER -