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Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids.

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Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids. / Mortezai, Naghmeh; Harder, Sönke; Schnabel, Claudia; Moors, Eva; Gauly, Matthias; Schlüter, Hartmut; Wagener, Christoph; Buck, Friedrich.

In: J PROTEOME RES, Vol. 9, No. 12, 12, 03.12.2010, p. 6126-6134.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Mortezai, N, Harder, S, Schnabel, C, Moors, E, Gauly, M, Schlüter, H, Wagener, C & Buck, F 2010, 'Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids.', J PROTEOME RES, vol. 9, no. 12, 12, pp. 6126-6134. https://doi.org/10.1021/pr100224y

APA

Mortezai, N., Harder, S., Schnabel, C., Moors, E., Gauly, M., Schlüter, H., Wagener, C., & Buck, F. (2010). Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids. J PROTEOME RES, 9(12), 6126-6134. [12]. https://doi.org/10.1021/pr100224y

Vancouver

Mortezai N, Harder S, Schnabel C, Moors E, Gauly M, Schlüter H et al. Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids. J PROTEOME RES. 2010 Dec 3;9(12):6126-6134. 12. https://doi.org/10.1021/pr100224y

Bibtex

@article{7b3d82f7d5c147bfb29e885980f12442,
title = "Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids.",
abstract = "Protein biomarker discovery in the low concentration range of human body fluids requires the enrichment of the proteins of interest. Here we report on a tandem affinity strategy: In the first step, we isolated a human plasma glyco-subproteome of healthy individuals by wheat germ agglutinin (WGA) lectin affinity chromatography. In the second step, the proteins of this subproteome were used to raise antibodies in llama (Lama glama). The heavy-chain fraction of the llama antibodies was used to deplete from the WGA lectin binding fraction all proteins normally found in human plasma. In this way, we selectively enriched the glycoprotein, CEA, a known cancer marker which had been spiked into normal plasma. As a proof of concept, we applied this method to the analysis of plasma sample from colon cancer patients. We could demonstrate the selective enrichment of CEA by a factor of 600-800.",
keywords = "Animals, Antibody Affinity, Biomarkers, Blotting, Western, Camelids, New World, Carcinoembryonic Antigen, Chromatography, Affinity, Chromatography, High Pressure Liquid, Colonic Neoplasms, Humans, Mass Spectrometry, Proteome, Proteomics, Reproducibility of Results, Wheat Germ Agglutinins, Journal Article",
author = "Naghmeh Mortezai and S{\"o}nke Harder and Claudia Schnabel and Eva Moors and Matthias Gauly and Hartmut Schl{\"u}ter and Christoph Wagener and Friedrich Buck",
year = "2010",
month = dec,
day = "3",
doi = "10.1021/pr100224y",
language = "English",
volume = "9",
pages = "6126--6134",
journal = "J PROTEOME RES",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "12",

}

RIS

TY - JOUR

T1 - Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids.

AU - Mortezai, Naghmeh

AU - Harder, Sönke

AU - Schnabel, Claudia

AU - Moors, Eva

AU - Gauly, Matthias

AU - Schlüter, Hartmut

AU - Wagener, Christoph

AU - Buck, Friedrich

PY - 2010/12/3

Y1 - 2010/12/3

N2 - Protein biomarker discovery in the low concentration range of human body fluids requires the enrichment of the proteins of interest. Here we report on a tandem affinity strategy: In the first step, we isolated a human plasma glyco-subproteome of healthy individuals by wheat germ agglutinin (WGA) lectin affinity chromatography. In the second step, the proteins of this subproteome were used to raise antibodies in llama (Lama glama). The heavy-chain fraction of the llama antibodies was used to deplete from the WGA lectin binding fraction all proteins normally found in human plasma. In this way, we selectively enriched the glycoprotein, CEA, a known cancer marker which had been spiked into normal plasma. As a proof of concept, we applied this method to the analysis of plasma sample from colon cancer patients. We could demonstrate the selective enrichment of CEA by a factor of 600-800.

AB - Protein biomarker discovery in the low concentration range of human body fluids requires the enrichment of the proteins of interest. Here we report on a tandem affinity strategy: In the first step, we isolated a human plasma glyco-subproteome of healthy individuals by wheat germ agglutinin (WGA) lectin affinity chromatography. In the second step, the proteins of this subproteome were used to raise antibodies in llama (Lama glama). The heavy-chain fraction of the llama antibodies was used to deplete from the WGA lectin binding fraction all proteins normally found in human plasma. In this way, we selectively enriched the glycoprotein, CEA, a known cancer marker which had been spiked into normal plasma. As a proof of concept, we applied this method to the analysis of plasma sample from colon cancer patients. We could demonstrate the selective enrichment of CEA by a factor of 600-800.

KW - Animals

KW - Antibody Affinity

KW - Biomarkers

KW - Blotting, Western

KW - Camelids, New World

KW - Carcinoembryonic Antigen

KW - Chromatography, Affinity

KW - Chromatography, High Pressure Liquid

KW - Colonic Neoplasms

KW - Humans

KW - Mass Spectrometry

KW - Proteome

KW - Proteomics

KW - Reproducibility of Results

KW - Wheat Germ Agglutinins

KW - Journal Article

U2 - 10.1021/pr100224y

DO - 10.1021/pr100224y

M3 - SCORING: Journal article

C2 - 20839810

VL - 9

SP - 6126

EP - 6134

JO - J PROTEOME RES

JF - J PROTEOME RES

SN - 1535-3893

IS - 12

M1 - 12

ER -