Research ExplorerPublicationsSyntaxin 11 is required for NK and CD8⁺ T-cell cytotoxicity ...

Syntaxin 11 is required for NK and CD8⁺ T-cell cytotoxicity and neutrophil degranulation.

Standard

Syntaxin 11 is required for NK and CD8⁺ T-cell cytotoxicity and neutrophil degranulation. / D'Orlando, Orietta; Zhao, Fang; Kasper, Brigitte; Orinska, Zane; Müller, Jürgen; Hermans-Borgmeyer, Irm; Griffiths, Gillian M; Zur Stadt, Udo; Bulfone-Paus, Silvia.

In: EUR J IMMUNOL, Vol. 43, No. 1, 1, 2013, p. 194-208.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

D'Orlando, O, Zhao, F, Kasper, B, Orinska, Z, Müller, J, Hermans-Borgmeyer, I, Griffiths, GM, Zur Stadt, U & Bulfone-Paus, S 2013, 'Syntaxin 11 is required for NK and CD8⁺ T-cell cytotoxicity and neutrophil degranulation.', EUR J IMMUNOL, vol. 43, no. 1, 1, pp. 194-208. https://doi.org/10.1002/eji.201142343

APA

D'Orlando, O., Zhao, F., Kasper, B., Orinska, Z., Müller, J., Hermans-Borgmeyer, I., Griffiths, G. M., Zur Stadt, U., & Bulfone-Paus, S. (2013). Syntaxin 11 is required for NK and CD8⁺ T-cell cytotoxicity and neutrophil degranulation. EUR J IMMUNOL, 43(1), 194-208. [1]. https://doi.org/10.1002/eji.201142343

Vancouver

D'Orlando O, Zhao F, Kasper B, Orinska Z, Müller J, Hermans-Borgmeyer I et al. Syntaxin 11 is required for NK and CD8⁺ T-cell cytotoxicity and neutrophil degranulation. EUR J IMMUNOL. 2013;43(1):194-208. 1. https://doi.org/10.1002/eji.201142343

Bibtex

@article{c60e61440c1f447095bb37b54c18585c,
title = "Syntaxin 11 is required for NK and CD8⁺ T-cell cytotoxicity and neutrophil degranulation.",
abstract = "Syntaxin 11 (STX11) controls vesicular trafficking and is a key player in exocytosis. Since Stx11 mutations are causally associated with a familial hemophagocytic lymphohistio-cytosis, we wanted to clarify whether STX11 is functionally important for key immune cell populations. This was studied in primary cells obtained from newly generated Stx11(-/-) mice. Our data revealed that STX11 is not only widely expressed in different immune cells, but also induced upon LPS or IFN-γ treatment. However, Stx11 deficiency does not affect macrophage phagocytic function and cytokine secretion, mast cell activation, or antigen presentation by DCs. Instead, STX11 selectively controls lymphocyte cytotoxicity in NK and activated CD8(+) T cells and degranulation in neutrophils. Stx11(-/-) NK cells and CTLs show impaired degranulation, despite a comparable activation, maturation and expression of the complex-forming partners MUNC18-2 and VTI1B. In addition, Stx11(-/-) CTLs and NK cells produce abnormal levels of IFN-γ. Since functional reconstitution rescues the defective phenotype of Stx11(-/-) CTLs, we suggest a direct, specific and key role of STX11 in controlling lymphocyte cytotoxicity, cytokine production and secretion. Finally, we show that these mice are a very useful tool for dissecting the role of STX11 in vesicular trafficking and secretion.",
keywords = "Animals, Humans, Mice, Mice, Inbred C57BL, Mice, Knockout, Cell Line, Mutation/genetics, Interferon-gamma/immunology, Neutrophils/*immunology, Killer Cells, Natural/*immunology, Cytotoxicity, Immunologic/genetics, CD8-Positive T-Lymphocytes/*immunology, Cell Degranulation/genetics, Lipopolysaccharides/immunology, Lymphohistiocytosis, Hemophagocytic/genetics/immunology, Munc18 Proteins/immunology, Qa-SNARE Proteins/genetics/*immunology, Qb-SNARE Proteins/immunology, Animals, Humans, Mice, Mice, Inbred C57BL, Mice, Knockout, Cell Line, Mutation/genetics, Interferon-gamma/immunology, Neutrophils/*immunology, Killer Cells, Natural/*immunology, Cytotoxicity, Immunologic/genetics, CD8-Positive T-Lymphocytes/*immunology, Cell Degranulation/genetics, Lipopolysaccharides/immunology, Lymphohistiocytosis, Hemophagocytic/genetics/immunology, Munc18 Proteins/immunology, Qa-SNARE Proteins/genetics/*immunology, Qb-SNARE Proteins/immunology",
author = "Orietta D'Orlando and Fang Zhao and Brigitte Kasper and Zane Orinska and J{\"u}rgen M{\"u}ller and Irm Hermans-Borgmeyer and Griffiths, {Gillian M} and {Zur Stadt}, Udo and Silvia Bulfone-Paus",
note = "{\textcopyright} 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2013",
doi = "10.1002/eji.201142343",
language = "English",
volume = "43",
pages = "194--208",
journal = "EUR J IMMUNOL",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag GmbH",
number = "1",

}

RIS

TY - JOUR

T1 - Syntaxin 11 is required for NK and CD8⁺ T-cell cytotoxicity and neutrophil degranulation.

AU - D'Orlando, Orietta

AU - Zhao, Fang

AU - Kasper, Brigitte

AU - Orinska, Zane

AU - Müller, Jürgen

AU - Hermans-Borgmeyer, Irm

AU - Griffiths, Gillian M

AU - Zur Stadt, Udo

AU - Bulfone-Paus, Silvia

N1 - © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2013

Y1 - 2013

N2 - Syntaxin 11 (STX11) controls vesicular trafficking and is a key player in exocytosis. Since Stx11 mutations are causally associated with a familial hemophagocytic lymphohistio-cytosis, we wanted to clarify whether STX11 is functionally important for key immune cell populations. This was studied in primary cells obtained from newly generated Stx11(-/-) mice. Our data revealed that STX11 is not only widely expressed in different immune cells, but also induced upon LPS or IFN-γ treatment. However, Stx11 deficiency does not affect macrophage phagocytic function and cytokine secretion, mast cell activation, or antigen presentation by DCs. Instead, STX11 selectively controls lymphocyte cytotoxicity in NK and activated CD8(+) T cells and degranulation in neutrophils. Stx11(-/-) NK cells and CTLs show impaired degranulation, despite a comparable activation, maturation and expression of the complex-forming partners MUNC18-2 and VTI1B. In addition, Stx11(-/-) CTLs and NK cells produce abnormal levels of IFN-γ. Since functional reconstitution rescues the defective phenotype of Stx11(-/-) CTLs, we suggest a direct, specific and key role of STX11 in controlling lymphocyte cytotoxicity, cytokine production and secretion. Finally, we show that these mice are a very useful tool for dissecting the role of STX11 in vesicular trafficking and secretion.

AB - Syntaxin 11 (STX11) controls vesicular trafficking and is a key player in exocytosis. Since Stx11 mutations are causally associated with a familial hemophagocytic lymphohistio-cytosis, we wanted to clarify whether STX11 is functionally important for key immune cell populations. This was studied in primary cells obtained from newly generated Stx11(-/-) mice. Our data revealed that STX11 is not only widely expressed in different immune cells, but also induced upon LPS or IFN-γ treatment. However, Stx11 deficiency does not affect macrophage phagocytic function and cytokine secretion, mast cell activation, or antigen presentation by DCs. Instead, STX11 selectively controls lymphocyte cytotoxicity in NK and activated CD8(+) T cells and degranulation in neutrophils. Stx11(-/-) NK cells and CTLs show impaired degranulation, despite a comparable activation, maturation and expression of the complex-forming partners MUNC18-2 and VTI1B. In addition, Stx11(-/-) CTLs and NK cells produce abnormal levels of IFN-γ. Since functional reconstitution rescues the defective phenotype of Stx11(-/-) CTLs, we suggest a direct, specific and key role of STX11 in controlling lymphocyte cytotoxicity, cytokine production and secretion. Finally, we show that these mice are a very useful tool for dissecting the role of STX11 in vesicular trafficking and secretion.

KW - Animals

KW - Humans

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Cell Line

KW - Mutation/genetics

KW - Interferon-gamma/immunology

KW - Neutrophils/immunology

KW - Killer Cells, Natural/immunology

KW - Cytotoxicity, Immunologic/genetics

KW - CD8-Positive T-Lymphocytes/immunology

KW - Cell Degranulation/genetics

KW - Lipopolysaccharides/immunology

KW - Lymphohistiocytosis, Hemophagocytic/genetics/immunology

KW - Munc18 Proteins/immunology

KW - Qa-SNARE Proteins/genetics/immunology

KW - Qb-SNARE Proteins/immunology

KW - Animals

KW - Humans

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Cell Line

KW - Mutation/genetics

KW - Interferon-gamma/immunology

KW - Neutrophils/immunology

KW - Killer Cells, Natural/immunology

KW - Cytotoxicity, Immunologic/genetics

KW - CD8-Positive T-Lymphocytes/immunology

KW - Cell Degranulation/genetics

KW - Lipopolysaccharides/immunology

KW - Lymphohistiocytosis, Hemophagocytic/genetics/immunology

KW - Munc18 Proteins/immunology

KW - Qa-SNARE Proteins/genetics/immunology

KW - Qb-SNARE Proteins/immunology

U2 - 10.1002/eji.201142343

DO - 10.1002/eji.201142343

M3 - SCORING: Journal article

C2 - 23042080

VL - 43

SP - 194

EP - 208

JO - EUR J IMMUNOL

JF - EUR J IMMUNOL

SN - 0014-2980

IS - 1

M1 - 1

ER -