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Superoxide production and expression of nox family proteins in human atherosclerosis

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Superoxide production and expression of nox family proteins in human atherosclerosis. / Sorescu, Dan; Weiss, Daiana; Lassègue, Bernard; Clempus, Roza E; Szöcs, Katalin; Sorescu, George P; Valppu, Liisa; Quinn, Mark T; Lambeth, J David; Vega, J David; Taylor, W Robert; Griendling, Kathy K.

In: CIRCULATION, Vol. 105, No. 12, 26.03.2002, p. 1429-1435.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Sorescu, D, Weiss, D, Lassègue, B, Clempus, RE, Szöcs, K, Sorescu, GP, Valppu, L, Quinn, MT, Lambeth, JD, Vega, JD, Taylor, WR & Griendling, KK 2002, 'Superoxide production and expression of nox family proteins in human atherosclerosis', CIRCULATION, vol. 105, no. 12, pp. 1429-1435. https://doi.org/10.1161/01.cir.0000012917.74432.66

APA

Sorescu, D., Weiss, D., Lassègue, B., Clempus, R. E., Szöcs, K., Sorescu, G. P., Valppu, L., Quinn, M. T., Lambeth, J. D., Vega, J. D., Taylor, W. R., & Griendling, K. K. (2002). Superoxide production and expression of nox family proteins in human atherosclerosis. CIRCULATION, 105(12), 1429-1435. https://doi.org/10.1161/01.cir.0000012917.74432.66

Vancouver

Sorescu D, Weiss D, Lassègue B, Clempus RE, Szöcs K, Sorescu GP et al. Superoxide production and expression of nox family proteins in human atherosclerosis. CIRCULATION. 2002 Mar 26;105(12):1429-1435. https://doi.org/10.1161/01.cir.0000012917.74432.66

Bibtex

@article{d85cec2eeb98496eac72ee4ae493bb8f,
title = "Superoxide production and expression of nox family proteins in human atherosclerosis",
abstract = "BACKGROUND: NAD(P)H oxidases are important sources of superoxide in the vasculature, the activity of which is associated with risk factors for human atherosclerosis. This study was designed to investigate the localization of superoxide production and the expression of the Nox family of NAD(P)H oxidase proteins (gp91phox, Nox1, and Nox4) in nonatherosclerotic and atherosclerotic human coronary arteries.METHODS AND RESULTS: In coronary artery segments from explanted human hearts, we examined intracellular superoxide production with dihydroethidium. In nonatherosclerotic coronary arteries, superoxide was present homogenously throughout the intima, media, and adventitia. In atherosclerotic arteries, there was an additional intense area of superoxide in the plaque shoulder, which is rich in macrophages and alpha-actin-positive cells. p22phox colocalized with gp91phox mainly in macrophages, whereas Nox4 was found only in nonphagocytic vascular cells. Expression of gp91phox and p22phox mRNA was associated with the severity of atherosclerosis. gp91phox correlated with the plaque macrophage content, whereas Nox4 correlated with the content of alpha-actin-positive cells. Nox1 expression was low both in human coronary arteries and isolated vascular cells.CONCLUSIONS: Several Nox proteins, including gp91phox and Nox4, may contribute to increased intracellular oxidative stress in human coronary atherosclerosis in a cell-specific manner and thus may be involved in the genesis and progression of human coronary atherosclerotic disease.",
keywords = "Arteriosclerosis/metabolism, Cells, Cultured, Coronary Artery Disease/metabolism, Coronary Vessels/chemistry, Endothelium, Vascular/cytology, Female, Fibroblasts/cytology, Fluorescent Antibody Technique, Fluorescent Dyes, Heart Failure/metabolism, Humans, Isoenzymes/genetics, Male, Membrane Glycoproteins/genetics, Membrane Transport Proteins, Middle Aged, Muscle, Smooth, Vascular/cytology, NADH, NADPH Oxidoreductases/biosynthesis, NADPH Dehydrogenase/genetics, NADPH Oxidase 2, NADPH Oxidase 4, NADPH Oxidases/genetics, Phosphoproteins/genetics, Polymerase Chain Reaction, RNA, Messenger/biosynthesis, Superoxides/analysis",
author = "Dan Sorescu and Daiana Weiss and Bernard Lass{\`e}gue and Clempus, {Roza E} and Katalin Sz{\"o}cs and Sorescu, {George P} and Liisa Valppu and Quinn, {Mark T} and Lambeth, {J David} and Vega, {J David} and Taylor, {W Robert} and Griendling, {Kathy K}",
year = "2002",
month = mar,
day = "26",
doi = "10.1161/01.cir.0000012917.74432.66",
language = "English",
volume = "105",
pages = "1429--1435",
journal = "CIRCULATION",
issn = "0009-7322",
publisher = "Lippincott Williams and Wilkins",
number = "12",

}

RIS

TY - JOUR

T1 - Superoxide production and expression of nox family proteins in human atherosclerosis

AU - Sorescu, Dan

AU - Weiss, Daiana

AU - Lassègue, Bernard

AU - Clempus, Roza E

AU - Szöcs, Katalin

AU - Sorescu, George P

AU - Valppu, Liisa

AU - Quinn, Mark T

AU - Lambeth, J David

AU - Vega, J David

AU - Taylor, W Robert

AU - Griendling, Kathy K

PY - 2002/3/26

Y1 - 2002/3/26

N2 - BACKGROUND: NAD(P)H oxidases are important sources of superoxide in the vasculature, the activity of which is associated with risk factors for human atherosclerosis. This study was designed to investigate the localization of superoxide production and the expression of the Nox family of NAD(P)H oxidase proteins (gp91phox, Nox1, and Nox4) in nonatherosclerotic and atherosclerotic human coronary arteries.METHODS AND RESULTS: In coronary artery segments from explanted human hearts, we examined intracellular superoxide production with dihydroethidium. In nonatherosclerotic coronary arteries, superoxide was present homogenously throughout the intima, media, and adventitia. In atherosclerotic arteries, there was an additional intense area of superoxide in the plaque shoulder, which is rich in macrophages and alpha-actin-positive cells. p22phox colocalized with gp91phox mainly in macrophages, whereas Nox4 was found only in nonphagocytic vascular cells. Expression of gp91phox and p22phox mRNA was associated with the severity of atherosclerosis. gp91phox correlated with the plaque macrophage content, whereas Nox4 correlated with the content of alpha-actin-positive cells. Nox1 expression was low both in human coronary arteries and isolated vascular cells.CONCLUSIONS: Several Nox proteins, including gp91phox and Nox4, may contribute to increased intracellular oxidative stress in human coronary atherosclerosis in a cell-specific manner and thus may be involved in the genesis and progression of human coronary atherosclerotic disease.

AB - BACKGROUND: NAD(P)H oxidases are important sources of superoxide in the vasculature, the activity of which is associated with risk factors for human atherosclerosis. This study was designed to investigate the localization of superoxide production and the expression of the Nox family of NAD(P)H oxidase proteins (gp91phox, Nox1, and Nox4) in nonatherosclerotic and atherosclerotic human coronary arteries.METHODS AND RESULTS: In coronary artery segments from explanted human hearts, we examined intracellular superoxide production with dihydroethidium. In nonatherosclerotic coronary arteries, superoxide was present homogenously throughout the intima, media, and adventitia. In atherosclerotic arteries, there was an additional intense area of superoxide in the plaque shoulder, which is rich in macrophages and alpha-actin-positive cells. p22phox colocalized with gp91phox mainly in macrophages, whereas Nox4 was found only in nonphagocytic vascular cells. Expression of gp91phox and p22phox mRNA was associated with the severity of atherosclerosis. gp91phox correlated with the plaque macrophage content, whereas Nox4 correlated with the content of alpha-actin-positive cells. Nox1 expression was low both in human coronary arteries and isolated vascular cells.CONCLUSIONS: Several Nox proteins, including gp91phox and Nox4, may contribute to increased intracellular oxidative stress in human coronary atherosclerosis in a cell-specific manner and thus may be involved in the genesis and progression of human coronary atherosclerotic disease.

KW - Arteriosclerosis/metabolism

KW - Cells, Cultured

KW - Coronary Artery Disease/metabolism

KW - Coronary Vessels/chemistry

KW - Endothelium, Vascular/cytology

KW - Female

KW - Fibroblasts/cytology

KW - Fluorescent Antibody Technique

KW - Fluorescent Dyes

KW - Heart Failure/metabolism

KW - Humans

KW - Isoenzymes/genetics

KW - Male

KW - Membrane Glycoproteins/genetics

KW - Membrane Transport Proteins

KW - Middle Aged

KW - Muscle, Smooth, Vascular/cytology

KW - NADH, NADPH Oxidoreductases/biosynthesis

KW - NADPH Dehydrogenase/genetics

KW - NADPH Oxidase 2

KW - NADPH Oxidase 4

KW - NADPH Oxidases/genetics

KW - Phosphoproteins/genetics

KW - Polymerase Chain Reaction

KW - RNA, Messenger/biosynthesis

KW - Superoxides/analysis

U2 - 10.1161/01.cir.0000012917.74432.66

DO - 10.1161/01.cir.0000012917.74432.66

M3 - SCORING: Journal article

C2 - 11914250

VL - 105

SP - 1429

EP - 1435

JO - CIRCULATION

JF - CIRCULATION

SN - 0009-7322

IS - 12

ER -