Subclassification of nerve sheath tumors by gene expression profiling
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Subclassification of nerve sheath tumors by gene expression profiling. / Holtkamp, Nikola; Reuss, David E; Atallah, Isis; Kuban, Ralf-Jürgen; Hartmann, Christian; Mautner, Victor-F; Frahm, Silke; Friedrich, Reinhard E; Algermissen, Bernd; Pham, Van-Anh; Prietz, Sandra; Rosenbaum, Thorsten; Estevez-Schwarz, Lope; von Deimling, Andreas.
In: BRAIN PATHOL, Vol. 14, No. 3, 01.07.2004, p. 258-64.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Subclassification of nerve sheath tumors by gene expression profiling
AU - Holtkamp, Nikola
AU - Reuss, David E
AU - Atallah, Isis
AU - Kuban, Ralf-Jürgen
AU - Hartmann, Christian
AU - Mautner, Victor-F
AU - Frahm, Silke
AU - Friedrich, Reinhard E
AU - Algermissen, Bernd
AU - Pham, Van-Anh
AU - Prietz, Sandra
AU - Rosenbaum, Thorsten
AU - Estevez-Schwarz, Lope
AU - von Deimling, Andreas
PY - 2004/7/1
Y1 - 2004/7/1
N2 - Nerve sheath tumors are the most common tumors of Neurofibromatosis type 1 (NF1) patients. Dermal neurofibromas develop in nearly all NF1-patients, whereas plexiform neurofibromas are only observed in one-third of the patients. NF1-patients have about a 10% lifetime risk for developing malignant pheripheral nerve sheath tumors (MPNST). The origin of these tumors is thought to be the Schwann cell lacking functional neurofibromin. However, additional genetic alterations are likely to modulate tumor biology and to contribute to individual nerve sheath tumor entities. To gain insight into the molecular events and to determine whether these tumors can be classified according to gene expression profiles, we performed expression analysis applying cDNA array technology. Nine dermal neurofibromas, 7 plexiform neurofibromas, ten MPNST and two MPNST cell cultures were examined. All tumors but 6 sporadic MPNST were obtained from NF1-patients. We detected significant differences in gene expression patterns between neurofibromas and MPNST and between dermal neurofibromas and plexiform neurofibromas. Tumor class prediction agreed in all but one case with histological and clinical classification. NF1-associated and sporadic MPNST could not be distinguished by their gene expression patterns. We present a panel of discriminating genes that may assist subclassification of nerve sheath tumors.
AB - Nerve sheath tumors are the most common tumors of Neurofibromatosis type 1 (NF1) patients. Dermal neurofibromas develop in nearly all NF1-patients, whereas plexiform neurofibromas are only observed in one-third of the patients. NF1-patients have about a 10% lifetime risk for developing malignant pheripheral nerve sheath tumors (MPNST). The origin of these tumors is thought to be the Schwann cell lacking functional neurofibromin. However, additional genetic alterations are likely to modulate tumor biology and to contribute to individual nerve sheath tumor entities. To gain insight into the molecular events and to determine whether these tumors can be classified according to gene expression profiles, we performed expression analysis applying cDNA array technology. Nine dermal neurofibromas, 7 plexiform neurofibromas, ten MPNST and two MPNST cell cultures were examined. All tumors but 6 sporadic MPNST were obtained from NF1-patients. We detected significant differences in gene expression patterns between neurofibromas and MPNST and between dermal neurofibromas and plexiform neurofibromas. Tumor class prediction agreed in all but one case with histological and clinical classification. NF1-associated and sporadic MPNST could not be distinguished by their gene expression patterns. We present a panel of discriminating genes that may assist subclassification of nerve sheath tumors.
KW - Adolescent
KW - Adult
KW - Aged
KW - Cell Line, Tumor
KW - Gene Expression
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Neoplastic
KW - Humans
KW - Image Processing, Computer-Assisted
KW - In Situ Hybridization
KW - Middle Aged
KW - Nerve Sheath Neoplasms
KW - Neurofibroma
KW - Neurofibroma, Plexiform
KW - Neurofibromatosis 1
KW - Oligonucleotide Array Sequence Analysis
M3 - SCORING: Journal article
C2 - 15446580
VL - 14
SP - 258
EP - 264
JO - BRAIN PATHOL
JF - BRAIN PATHOL
SN - 1015-6305
IS - 3
ER -