Structure of the murine tenascin-R gene and functional characterisation of the promoter

TY - JOUR

T1 - Structure of the murine tenascin-R gene and functional characterisation of the promoter

AU - Putthoff, Peggy

AU - Akyüz, Nuray

AU - Kutsche, Michael

AU - Zardi, Luciano

AU - Borgmeyer, Uwe

AU - Schachner, Melitta

PY - 2003/9/5

Y1 - 2003/9/5

N2 - The tenascin-R (TN-R) gene encodes a multidomain extracellular matrix glycoprotein belonging to the tenascin family. It is detectable mainly in oligodendrocytes and neuronal subpopulations of the central nervous system. In this report, we describe the structure of the 5'-region of the mouse TN-R gene and characterise the activity of its promoter. By in silico cloning and genome walking, we have deduced the organisation of the gene and identified the promoter sequence by 5'-RACE technology. TN-R transcripts in adult mouse brain contain non-coding exons 1 and 2 as demonstrated by the reverse transcriptase-polymerase chain reaction. The promoter displays its activity in cultured cells of neural origin, but not in a fibroblast-like cell line or an undifferentiated teratocarcimoma cell line. As for the human and rat genes, the elements required for the full and cell type-specific activity of the promoter are contained in exon 1 and 167 bp upstream of this exon. The mouse TN-R promoter sequence is similar to that of rat and human in that it displays similarly unusual features: it lacks any classical TATA-box or CAAT-box, GC-rich regions or initiator elements. The promoter contains consensus sequences for binding of a variety of transcription factors, notably p53/p73 and glucocorticoid receptors.

AB - The tenascin-R (TN-R) gene encodes a multidomain extracellular matrix glycoprotein belonging to the tenascin family. It is detectable mainly in oligodendrocytes and neuronal subpopulations of the central nervous system. In this report, we describe the structure of the 5'-region of the mouse TN-R gene and characterise the activity of its promoter. By in silico cloning and genome walking, we have deduced the organisation of the gene and identified the promoter sequence by 5'-RACE technology. TN-R transcripts in adult mouse brain contain non-coding exons 1 and 2 as demonstrated by the reverse transcriptase-polymerase chain reaction. The promoter displays its activity in cultured cells of neural origin, but not in a fibroblast-like cell line or an undifferentiated teratocarcimoma cell line. As for the human and rat genes, the elements required for the full and cell type-specific activity of the promoter are contained in exon 1 and 167 bp upstream of this exon. The mouse TN-R promoter sequence is similar to that of rat and human in that it displays similarly unusual features: it lacks any classical TATA-box or CAAT-box, GC-rich regions or initiator elements. The promoter contains consensus sequences for binding of a variety of transcription factors, notably p53/p73 and glucocorticoid receptors.

KW - Animals

KW - Base Sequence

KW - Brain

KW - Cell Differentiation

KW - Cloning, Molecular

KW - DNA, Complementary

KW - DNA-Binding Proteins

KW - Exons

KW - Fibroblasts

KW - Genes, Tumor Suppressor

KW - Genome

KW - Humans

KW - Mice

KW - Mice, Inbred C57BL

KW - Models, Genetic

KW - Molecular Sequence Data

KW - Neurons

KW - Nuclear Proteins

KW - Polymerase Chain Reaction

KW - Promoter Regions, Genetic

KW - Protein Binding

KW - RNA, Messenger

KW - Rats

KW - Receptors, Glucocorticoid

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Homology, Nucleic Acid

KW - Species Specificity

KW - Tenascin

KW - Transcription, Genetic

KW - Transfection

KW - Tumor Cells, Cultured

KW - Tumor Suppressor Protein p53

KW - Tumor Suppressor Proteins

M3 - SCORING: Journal article

C2 - 12927810

VL - 308

SP - 940

EP - 949

JO - BIOCHEM BIOPH RES CO

JF - BIOCHEM BIOPH RES CO

SN - 0006-291X

IS - 4

ER -