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Stra13, a prostaglandin E2-induced gene, regulates the cellular redox state of podocytes

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Stra13, a prostaglandin E2-induced gene, regulates the cellular redox state of podocytes. / Bek, Martin J; Wahle, Stephanie; Müller, Barbara; Benzing, Thomas; Huber, Tobias B; Kretzler, Matthias; Cohen, Clemen; Busse-Grawitz, Andrea; Pavenstädt, Hermann.

In: FASEB J, Vol. 17, No. 6, 04.2003, p. 682-4.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bek, MJ, Wahle, S, Müller, B, Benzing, T, Huber, TB, Kretzler, M, Cohen, C, Busse-Grawitz, A & Pavenstädt, H 2003, 'Stra13, a prostaglandin E2-induced gene, regulates the cellular redox state of podocytes', FASEB J, vol. 17, no. 6, pp. 682-4. https://doi.org/10.1096/fj.02-0250fje

APA

Bek, M. J., Wahle, S., Müller, B., Benzing, T., Huber, T. B., Kretzler, M., Cohen, C., Busse-Grawitz, A., & Pavenstädt, H. (2003). Stra13, a prostaglandin E2-induced gene, regulates the cellular redox state of podocytes. FASEB J, 17(6), 682-4. https://doi.org/10.1096/fj.02-0250fje

Vancouver

Bek MJ, Wahle S, Müller B, Benzing T, Huber TB, Kretzler M et al. Stra13, a prostaglandin E2-induced gene, regulates the cellular redox state of podocytes. FASEB J. 2003 Apr;17(6):682-4. https://doi.org/10.1096/fj.02-0250fje

Bibtex

@article{ca8d53ac071642ee88665195c9f12413,
title = "Stra13, a prostaglandin E2-induced gene, regulates the cellular redox state of podocytes",
abstract = "Podocyte injury is a central mechanism in the pathogenesis of proteinuria. Prostaglandin E2 (PGE2) has been suggested to protect podocytes from cellular injury. Here we investigated whether PGE2-induced gene expression accounts for the protective role of PGE2 in podocytes. Using a suppressive-subtractive hybridization method, we isolated a differentially expressed clone that was identified as Stra13, a recently described retinoic acid-inducible gene. PGE2, forskolin, and retinoic acid induced a time-dependent up-regulation of Stra13 mRNA and protein expression in podocytes. To test the function of Stra13 in podocytes, Stra13 was overexpressed by using retroviral gene transfer. Compared with control cells, cells overexpressing Stra13 showed markedly reduced NADPH-dependent superoxid anion generation. Furthermore, expression of heme oxygenase 1 (HO-1) was increased in podocytes overexpressing Stra13. HO-1 plays an important protective role in the defense against reactive oxygen species (ROS). After stimulation with exogenous ROS, Stra13-overexpressing podocytes were more resistant to oxidative stress than were control cells. Our data indicate that Stra13 may play an important protective role against oxidative stress in podocytes. ROS are involved in the pathogenesis of glomerular inflammation in several forms of glomerulonephritis. Therefore, knowledge about protective mechanisms may provide insight into new therapeutic strategies for glomerulopathies.",
keywords = "Animals, Basic Helix-Loop-Helix Transcription Factors, Blotting, Western, Colforsin, DNA, Antisense, Dinoprostone, Gene Expression Regulation, Heme Oxygenase (Decyclizing), Heme Oxygenase-1, Homeodomain Proteins, Kidney Glomerulus, Membrane Proteins, Mice, NADPH Oxidase, Oxidation-Reduction, RNA, Messenger, Reactive Oxygen Species, Tretinoin, Journal Article",
author = "Bek, {Martin J} and Stephanie Wahle and Barbara M{\"u}ller and Thomas Benzing and Huber, {Tobias B} and Matthias Kretzler and Clemen Cohen and Andrea Busse-Grawitz and Hermann Pavenst{\"a}dt",
year = "2003",
month = apr,
doi = "10.1096/fj.02-0250fje",
language = "English",
volume = "17",
pages = "682--4",
journal = "FASEB J",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

RIS

TY - JOUR

T1 - Stra13, a prostaglandin E2-induced gene, regulates the cellular redox state of podocytes

AU - Bek, Martin J

AU - Wahle, Stephanie

AU - Müller, Barbara

AU - Benzing, Thomas

AU - Huber, Tobias B

AU - Kretzler, Matthias

AU - Cohen, Clemen

AU - Busse-Grawitz, Andrea

AU - Pavenstädt, Hermann

PY - 2003/4

Y1 - 2003/4

N2 - Podocyte injury is a central mechanism in the pathogenesis of proteinuria. Prostaglandin E2 (PGE2) has been suggested to protect podocytes from cellular injury. Here we investigated whether PGE2-induced gene expression accounts for the protective role of PGE2 in podocytes. Using a suppressive-subtractive hybridization method, we isolated a differentially expressed clone that was identified as Stra13, a recently described retinoic acid-inducible gene. PGE2, forskolin, and retinoic acid induced a time-dependent up-regulation of Stra13 mRNA and protein expression in podocytes. To test the function of Stra13 in podocytes, Stra13 was overexpressed by using retroviral gene transfer. Compared with control cells, cells overexpressing Stra13 showed markedly reduced NADPH-dependent superoxid anion generation. Furthermore, expression of heme oxygenase 1 (HO-1) was increased in podocytes overexpressing Stra13. HO-1 plays an important protective role in the defense against reactive oxygen species (ROS). After stimulation with exogenous ROS, Stra13-overexpressing podocytes were more resistant to oxidative stress than were control cells. Our data indicate that Stra13 may play an important protective role against oxidative stress in podocytes. ROS are involved in the pathogenesis of glomerular inflammation in several forms of glomerulonephritis. Therefore, knowledge about protective mechanisms may provide insight into new therapeutic strategies for glomerulopathies.

AB - Podocyte injury is a central mechanism in the pathogenesis of proteinuria. Prostaglandin E2 (PGE2) has been suggested to protect podocytes from cellular injury. Here we investigated whether PGE2-induced gene expression accounts for the protective role of PGE2 in podocytes. Using a suppressive-subtractive hybridization method, we isolated a differentially expressed clone that was identified as Stra13, a recently described retinoic acid-inducible gene. PGE2, forskolin, and retinoic acid induced a time-dependent up-regulation of Stra13 mRNA and protein expression in podocytes. To test the function of Stra13 in podocytes, Stra13 was overexpressed by using retroviral gene transfer. Compared with control cells, cells overexpressing Stra13 showed markedly reduced NADPH-dependent superoxid anion generation. Furthermore, expression of heme oxygenase 1 (HO-1) was increased in podocytes overexpressing Stra13. HO-1 plays an important protective role in the defense against reactive oxygen species (ROS). After stimulation with exogenous ROS, Stra13-overexpressing podocytes were more resistant to oxidative stress than were control cells. Our data indicate that Stra13 may play an important protective role against oxidative stress in podocytes. ROS are involved in the pathogenesis of glomerular inflammation in several forms of glomerulonephritis. Therefore, knowledge about protective mechanisms may provide insight into new therapeutic strategies for glomerulopathies.

KW - Animals

KW - Basic Helix-Loop-Helix Transcription Factors

KW - Blotting, Western

KW - Colforsin

KW - DNA, Antisense

KW - Dinoprostone

KW - Gene Expression Regulation

KW - Heme Oxygenase (Decyclizing)

KW - Heme Oxygenase-1

KW - Homeodomain Proteins

KW - Kidney Glomerulus

KW - Membrane Proteins

KW - Mice

KW - NADPH Oxidase

KW - Oxidation-Reduction

KW - RNA, Messenger

KW - Reactive Oxygen Species

KW - Tretinoin

KW - Journal Article

U2 - 10.1096/fj.02-0250fje

DO - 10.1096/fj.02-0250fje

M3 - SCORING: Journal article

C2 - 12594185

VL - 17

SP - 682

EP - 684

JO - FASEB J

JF - FASEB J

SN - 0892-6638

IS - 6

ER -