Stimulation of Local Cytosolic Calcium Release by Photothermal Heating for Studying Intra- and Intercellular Calcium Waves
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Stimulation of Local Cytosolic Calcium Release by Photothermal Heating for Studying Intra- and Intercellular Calcium Waves. / Zhu, Dingcheng; Feng, Lucy; Feliu, Neus; Guse, Andreas H.; Parak, Wolfgang J.
In: ADV MATER, Vol. 33, No. 24, 06.2021, p. e2008261.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Stimulation of Local Cytosolic Calcium Release by Photothermal Heating for Studying Intra- and Intercellular Calcium Waves
AU - Zhu, Dingcheng
AU - Feng, Lucy
AU - Feliu, Neus
AU - Guse, Andreas H.
AU - Parak, Wolfgang J
N1 - © 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.
PY - 2021/6
Y1 - 2021/6
N2 - A methodology is described that allows for localized Ca2+ release by photoexcitation. For this, cells are loaded with polymer capsules with integrated plasmonic nanoparticles, which reside in endo-lysosomes. The micrometer-sized capsules can be individually excited by near-infrared light from a light pointer, causing photothermal heating, upon which there is a rise in the free cytosolic Ca2+ concentration ([Ca2+ ]i ). The [Ca2+ ]i can be analyzed with a Ca2+ indicator fluorophore. In this way, it is possible to excite local lysosomal Ca2+ release in a desired target cell.
AB - A methodology is described that allows for localized Ca2+ release by photoexcitation. For this, cells are loaded with polymer capsules with integrated plasmonic nanoparticles, which reside in endo-lysosomes. The micrometer-sized capsules can be individually excited by near-infrared light from a light pointer, causing photothermal heating, upon which there is a rise in the free cytosolic Ca2+ concentration ([Ca2+ ]i ). The [Ca2+ ]i can be analyzed with a Ca2+ indicator fluorophore. In this way, it is possible to excite local lysosomal Ca2+ release in a desired target cell.
U2 - 10.1002/adma.202008261
DO - 10.1002/adma.202008261
M3 - SCORING: Journal article
C2 - 33949733
VL - 33
SP - e2008261
JO - ADV MATER
JF - ADV MATER
SN - 0935-9648
IS - 24
ER -