Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels

J Simon Wiegert; Christine E Gee; Thomas G Oertner

doi:10.1101/pdb.top089714

Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels

Standard

Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels. / Wiegert, J Simon; Gee, Christine E ; Oertner, Thomas G.

In: Cold Spring Harbor protocols, Vol. 2017, No. 2, 01.02.2017, p. pdb.top089714.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wiegert, JS, Gee, CE & Oertner, TG 2017, 'Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels', Cold Spring Harbor protocols, vol. 2017, no. 2, pp. pdb.top089714. https://doi.org/10.1101/pdb.top089714

APA

Wiegert, J. S., Gee, C. E., & Oertner, T. G. (2017). Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels. Cold Spring Harbor protocols, 2017(2), pdb.top089714. https://doi.org/10.1101/pdb.top089714

Vancouver

Wiegert JS, Gee CE , Oertner TG. Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels. Cold Spring Harbor protocols. 2017 Feb 1;2017(2):pdb.top089714. https://doi.org/10.1101/pdb.top089714

Bibtex

@article{48b72324ffaa4d139edad28976986b12,

title = "Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels",

abstract = "Heterologous expression of ion channels that can be directly gated by light has made it possible to stimulate almost any excitable cell with light. Optogenetic stimulation has been particularly powerful in the neurosciences, as it allows the activation of specific, genetically defined neurons with precise timing. Organotypic hippocampal slice cultures are a favored preparation for optogenetic experiments. They can be cultured for many weeks and, after transfection with optogenetic actuators and sensors, allow the study of individual synapses or small networks. The absence of any electrodes allows multiple imaging sessions over the course of several days and even chronic stimulation inside the incubator. These timescales are not accessible in electrophysiological experiments. Here, we introduce the production of organotypic hippocampal slice cultures and their transduction or transfection with optogenetic tools. We then discuss the options for light stimulation.",

author = "Wiegert, {J Simon} and Gee, {Christine E} and Oertner, {Thomas G}",

note = "{\textcopyright} 2017 Cold Spring Harbor Laboratory Press.",

year = "2017",

month = feb,

day = "1",

doi = "10.1101/pdb.top089714",

language = "English",

volume = "2017",

pages = "pdb.top089714",

journal = "Cold Spring Harbor protocols",

issn = "1559-6095",

publisher = "Cold Spring Harbor Laboratory Press",

number = "2",

}

RIS

TY - JOUR

T1 - Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels

AU - Wiegert, J Simon

AU - Gee, Christine E

AU - Oertner, Thomas G

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Heterologous expression of ion channels that can be directly gated by light has made it possible to stimulate almost any excitable cell with light. Optogenetic stimulation has been particularly powerful in the neurosciences, as it allows the activation of specific, genetically defined neurons with precise timing. Organotypic hippocampal slice cultures are a favored preparation for optogenetic experiments. They can be cultured for many weeks and, after transfection with optogenetic actuators and sensors, allow the study of individual synapses or small networks. The absence of any electrodes allows multiple imaging sessions over the course of several days and even chronic stimulation inside the incubator. These timescales are not accessible in electrophysiological experiments. Here, we introduce the production of organotypic hippocampal slice cultures and their transduction or transfection with optogenetic tools. We then discuss the options for light stimulation.

AB - Heterologous expression of ion channels that can be directly gated by light has made it possible to stimulate almost any excitable cell with light. Optogenetic stimulation has been particularly powerful in the neurosciences, as it allows the activation of specific, genetically defined neurons with precise timing. Organotypic hippocampal slice cultures are a favored preparation for optogenetic experiments. They can be cultured for many weeks and, after transfection with optogenetic actuators and sensors, allow the study of individual synapses or small networks. The absence of any electrodes allows multiple imaging sessions over the course of several days and even chronic stimulation inside the incubator. These timescales are not accessible in electrophysiological experiments. Here, we introduce the production of organotypic hippocampal slice cultures and their transduction or transfection with optogenetic tools. We then discuss the options for light stimulation.

U2 - 10.1101/pdb.top089714

DO - 10.1101/pdb.top089714

M3 - SCORING: Journal article

C2 - 28148885

VL - 2017

SP - pdb.top089714

JO - Cold Spring Harbor protocols

JF - Cold Spring Harbor protocols

SN - 1559-6095

IS - 2

ER -