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Staining of endothelial cells does not change the result of cell density

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Staining of endothelial cells does not change the result of cell density. / Wenzel, Daniel A; Kunzmann, Berenike C; Spitzer, Martin S; Schultheiss, Maximilian.

In: CELL TISSUE BANK, Vol. 20, No. 2, 06.2019, p. 327-328.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wenzel, DA, Kunzmann, BC, Spitzer, MS & Schultheiss, M 2019, 'Staining of endothelial cells does not change the result of cell density', CELL TISSUE BANK, vol. 20, no. 2, pp. 327-328. https://doi.org/10.1007/s10561-019-09759-6

APA

Wenzel, D. A., Kunzmann, B. C., Spitzer, M. S., & Schultheiss, M. (2019). Staining of endothelial cells does not change the result of cell density. CELL TISSUE BANK, 20(2), 327-328. https://doi.org/10.1007/s10561-019-09759-6

Vancouver

Wenzel DA, Kunzmann BC, Spitzer MS, Schultheiss M. Staining of endothelial cells does not change the result of cell density. CELL TISSUE BANK. 2019 Jun;20(2):327-328. https://doi.org/10.1007/s10561-019-09759-6

Bibtex

@article{9dec82aed4d04140b051a0e559cbddb2,
title = "Staining of endothelial cells does not change the result of cell density",
abstract = "To determine the corneal endothelial cell density human donor corneas are usually placed in hypotonic balanced salt solution for unstained cell counting. Experimental studies often stain the corneal endothelium to highlight cells and cell borders and simplify counting. However, staining normally terminates the experiments. Up to date, there is no publication comparing endothelial cell counts before and after staining. This study compared the counting results of unstained (hypotonic balanced salt solution) and stained (trypan blue & alizarin red S) assessment of the corneal endothelial cell density. No evidence for significantly differing cell counts was found between unstained and stained analysis of the corneal endothelial cell density. Therefore, we consider unstained counting non-inferior to stained counting.",
author = "Wenzel, {Daniel A} and Kunzmann, {Berenike C} and Spitzer, {Martin S} and Maximilian Schultheiss",
year = "2019",
month = jun,
doi = "10.1007/s10561-019-09759-6",
language = "English",
volume = "20",
pages = "327--328",
journal = "CELL TISSUE BANK",
issn = "1389-9333",
publisher = "Springer Netherlands",
number = "2",

}

RIS

TY - JOUR

T1 - Staining of endothelial cells does not change the result of cell density

AU - Wenzel, Daniel A

AU - Kunzmann, Berenike C

AU - Spitzer, Martin S

AU - Schultheiss, Maximilian

PY - 2019/6

Y1 - 2019/6

N2 - To determine the corneal endothelial cell density human donor corneas are usually placed in hypotonic balanced salt solution for unstained cell counting. Experimental studies often stain the corneal endothelium to highlight cells and cell borders and simplify counting. However, staining normally terminates the experiments. Up to date, there is no publication comparing endothelial cell counts before and after staining. This study compared the counting results of unstained (hypotonic balanced salt solution) and stained (trypan blue & alizarin red S) assessment of the corneal endothelial cell density. No evidence for significantly differing cell counts was found between unstained and stained analysis of the corneal endothelial cell density. Therefore, we consider unstained counting non-inferior to stained counting.

AB - To determine the corneal endothelial cell density human donor corneas are usually placed in hypotonic balanced salt solution for unstained cell counting. Experimental studies often stain the corneal endothelium to highlight cells and cell borders and simplify counting. However, staining normally terminates the experiments. Up to date, there is no publication comparing endothelial cell counts before and after staining. This study compared the counting results of unstained (hypotonic balanced salt solution) and stained (trypan blue & alizarin red S) assessment of the corneal endothelial cell density. No evidence for significantly differing cell counts was found between unstained and stained analysis of the corneal endothelial cell density. Therefore, we consider unstained counting non-inferior to stained counting.

U2 - 10.1007/s10561-019-09759-6

DO - 10.1007/s10561-019-09759-6

M3 - SCORING: Journal article

C2 - 30810847

VL - 20

SP - 327

EP - 328

JO - CELL TISSUE BANK

JF - CELL TISSUE BANK

SN - 1389-9333

IS - 2

ER -