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Stage-dependent increase of orosomucoid and zinc-alpha2-glycoprotein in urinary bladder cancer.

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Stage-dependent increase of orosomucoid and zinc-alpha2-glycoprotein in urinary bladder cancer. / Irmak, Ster; Tilki, Derya; Heukeshoven, Jochen; Oliveira-Ferrer, Leticia; Friedrich, Martin; Huland, Hartwig; Ergün, Süleyman.

In: PROTEOMICS, Vol. 5, No. 16, 16, 2005, p. 4296-4304.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Irmak, S, Tilki, D, Heukeshoven, J, Oliveira-Ferrer, L, Friedrich, M, Huland, H & Ergün, S 2005, 'Stage-dependent increase of orosomucoid and zinc-alpha2-glycoprotein in urinary bladder cancer.', PROTEOMICS, vol. 5, no. 16, 16, pp. 4296-4304. <http://www.ncbi.nlm.nih.gov/pubmed/16196100?dopt=Citation>

APA

Irmak, S., Tilki, D., Heukeshoven, J., Oliveira-Ferrer, L., Friedrich, M., Huland, H., & Ergün, S. (2005). Stage-dependent increase of orosomucoid and zinc-alpha2-glycoprotein in urinary bladder cancer. PROTEOMICS, 5(16), 4296-4304. [16]. http://www.ncbi.nlm.nih.gov/pubmed/16196100?dopt=Citation

Vancouver

Irmak S, Tilki D, Heukeshoven J, Oliveira-Ferrer L, Friedrich M, Huland H et al. Stage-dependent increase of orosomucoid and zinc-alpha2-glycoprotein in urinary bladder cancer. PROTEOMICS. 2005;5(16):4296-4304. 16.

Bibtex

@article{51e296b04ad14d9d9390678bc0409da0,
title = "Stage-dependent increase of orosomucoid and zinc-alpha2-glycoprotein in urinary bladder cancer.",
abstract = "Identification and characterization of biomarkers in body fluids such as serum or urine serve as a basis for early detection of diseases, particularly of cancer. Performing 2-DE with subsequent MS analyses, conventional immunoblotting and immunohistochemistry we identified two proteins, orosomucoid (ORM) and human zinc-alpha(2)-glycoprotein (ZAG), which were increased in the urine samples of patients with bladder cancer in comparison to the urine samples of healthy volunteers. The highest amount of both proteins was found in invasive bladder cancer stages such as pT2-3. Immunohistochemical studies showed ORM in inflammatory cells but also in endothelial cells of blood vessels within or adjacent to the tumor area and in part of the tumor cells. ZAG was prominent in tumor cells at the tumor invasion front. Additionally, ZAG was localized at the luminal surface of normal urothelium, which switches to the basal side when a superficial papillary tumor was observed. These results show that we have been able to identify two new proteins that may be related to the development of superficial bladder cancer and to its switch to an invasive phenotype.",
author = "Ster Irmak and Derya Tilki and Jochen Heukeshoven and Leticia Oliveira-Ferrer and Martin Friedrich and Hartwig Huland and S{\"u}leyman Erg{\"u}n",
year = "2005",
language = "Deutsch",
volume = "5",
pages = "4296--4304",
journal = "PROTEOMICS",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag GmbH",
number = "16",

}

RIS

TY - JOUR

T1 - Stage-dependent increase of orosomucoid and zinc-alpha2-glycoprotein in urinary bladder cancer.

AU - Irmak, Ster

AU - Tilki, Derya

AU - Heukeshoven, Jochen

AU - Oliveira-Ferrer, Leticia

AU - Friedrich, Martin

AU - Huland, Hartwig

AU - Ergün, Süleyman

PY - 2005

Y1 - 2005

N2 - Identification and characterization of biomarkers in body fluids such as serum or urine serve as a basis for early detection of diseases, particularly of cancer. Performing 2-DE with subsequent MS analyses, conventional immunoblotting and immunohistochemistry we identified two proteins, orosomucoid (ORM) and human zinc-alpha(2)-glycoprotein (ZAG), which were increased in the urine samples of patients with bladder cancer in comparison to the urine samples of healthy volunteers. The highest amount of both proteins was found in invasive bladder cancer stages such as pT2-3. Immunohistochemical studies showed ORM in inflammatory cells but also in endothelial cells of blood vessels within or adjacent to the tumor area and in part of the tumor cells. ZAG was prominent in tumor cells at the tumor invasion front. Additionally, ZAG was localized at the luminal surface of normal urothelium, which switches to the basal side when a superficial papillary tumor was observed. These results show that we have been able to identify two new proteins that may be related to the development of superficial bladder cancer and to its switch to an invasive phenotype.

AB - Identification and characterization of biomarkers in body fluids such as serum or urine serve as a basis for early detection of diseases, particularly of cancer. Performing 2-DE with subsequent MS analyses, conventional immunoblotting and immunohistochemistry we identified two proteins, orosomucoid (ORM) and human zinc-alpha(2)-glycoprotein (ZAG), which were increased in the urine samples of patients with bladder cancer in comparison to the urine samples of healthy volunteers. The highest amount of both proteins was found in invasive bladder cancer stages such as pT2-3. Immunohistochemical studies showed ORM in inflammatory cells but also in endothelial cells of blood vessels within or adjacent to the tumor area and in part of the tumor cells. ZAG was prominent in tumor cells at the tumor invasion front. Additionally, ZAG was localized at the luminal surface of normal urothelium, which switches to the basal side when a superficial papillary tumor was observed. These results show that we have been able to identify two new proteins that may be related to the development of superficial bladder cancer and to its switch to an invasive phenotype.

M3 - SCORING: Zeitschriftenaufsatz

VL - 5

SP - 4296

EP - 4304

JO - PROTEOMICS

JF - PROTEOMICS

SN - 1615-9853

IS - 16

M1 - 16

ER -