Stable isotope dilution assay for liquid chromatography-tandem mass spectrometric determination of L-homoarginine in human plasma.
Standard
Stable isotope dilution assay for liquid chromatography-tandem mass spectrometric determination of L-homoarginine in human plasma. / Atzler, Dorothee; Mieth, Maren; Maas, Renke; Böger, Rainer; Schwedhelm, Edzard.
In: J CHROMATOGR B, Vol. 879, No. 23, 23, 2011, p. 2294-2298.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Stable isotope dilution assay for liquid chromatography-tandem mass spectrometric determination of L-homoarginine in human plasma.
AU - Atzler, Dorothee
AU - Mieth, Maren
AU - Maas, Renke
AU - Böger, Rainer
AU - Schwedhelm, Edzard
PY - 2011
Y1 - 2011
N2 - Nitric oxide (NO), the endogenous modulator of vascular tone and structure, originates from oxidation of L-arginine catalysed by NO synthase (NOS). The L-arginine derivative L-homoarginine serves as an alternative NOS substrate releasing NO, competing with L-arginine for NOS, arginase, and arginine transport. In the present article we report a liquid chromatography-tandem mass spectrometric (LC-tandem MS) method for the determination of L-homoarginine in human plasma by stable-isotope dilution. L-[(13)C(6)]-Homoarginine was used as internal standard. This method provides high sample throughput of 25-?l aliquots of plasma with an analysis time of 4 min using LC-tandem MS electrospray ionisation in the positive mode (ESI+). Specific transitions for L-homoarginine and L-[(13)C(6)]-homoarginine were m/z 245 ? m/z 211 and m/z 251 ? m/z 217, respectively. The mean intra- and interassay CVs were 7.4 ± 4.5% (±SD) for 0.1-50 ?mol/L and 7.5 ± 2.0% for 2 and 5 ?mol/L, respectively. Applying this method, a mean plasma concentration of L-homoarginine of 2.5 ± 1.0 ?mol/L was determined in 136 healthy humans.
AB - Nitric oxide (NO), the endogenous modulator of vascular tone and structure, originates from oxidation of L-arginine catalysed by NO synthase (NOS). The L-arginine derivative L-homoarginine serves as an alternative NOS substrate releasing NO, competing with L-arginine for NOS, arginase, and arginine transport. In the present article we report a liquid chromatography-tandem mass spectrometric (LC-tandem MS) method for the determination of L-homoarginine in human plasma by stable-isotope dilution. L-[(13)C(6)]-Homoarginine was used as internal standard. This method provides high sample throughput of 25-?l aliquots of plasma with an analysis time of 4 min using LC-tandem MS electrospray ionisation in the positive mode (ESI+). Specific transitions for L-homoarginine and L-[(13)C(6)]-homoarginine were m/z 245 ? m/z 211 and m/z 251 ? m/z 217, respectively. The mean intra- and interassay CVs were 7.4 ± 4.5% (±SD) for 0.1-50 ?mol/L and 7.5 ± 2.0% for 2 and 5 ?mol/L, respectively. Applying this method, a mean plasma concentration of L-homoarginine of 2.5 ± 1.0 ?mol/L was determined in 136 healthy humans.
KW - Humans
KW - Carbon Isotopes/blood
KW - Chromatography, Liquid/methods
KW - Homoarginine/blood
KW - Indicator Dilution Techniques
KW - Tandem Mass Spectrometry/methods
KW - Humans
KW - Carbon Isotopes/blood
KW - Chromatography, Liquid/methods
KW - Homoarginine/blood
KW - Indicator Dilution Techniques
KW - Tandem Mass Spectrometry/methods
M3 - SCORING: Journal article
VL - 879
SP - 2294
EP - 2298
JO - J CHROMATOGR B
JF - J CHROMATOGR B
SN - 1570-0232
IS - 23
M1 - 23
ER -
