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Spinal afferent neurons projecting to the rat lung and pleura express acid sensitive channels

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Spinal afferent neurons projecting to the rat lung and pleura express acid sensitive channels. / Groth, Michael; Helbig, Tanja; Grau, Veronika; Kummer, Wolfgang; Haberberger, Rainer V.

In: RESP RES, Vol. 7, 2006, p. 96.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Groth, M, Helbig, T, Grau, V, Kummer, W & Haberberger, RV 2006, 'Spinal afferent neurons projecting to the rat lung and pleura express acid sensitive channels', RESP RES, vol. 7, pp. 96. https://doi.org/10.1186/1465-9921-7-96

APA

Groth, M., Helbig, T., Grau, V., Kummer, W., & Haberberger, R. V. (2006). Spinal afferent neurons projecting to the rat lung and pleura express acid sensitive channels. RESP RES, 7, 96. https://doi.org/10.1186/1465-9921-7-96

Vancouver

Groth M, Helbig T, Grau V, Kummer W, Haberberger RV. Spinal afferent neurons projecting to the rat lung and pleura express acid sensitive channels. RESP RES. 2006;7:96. https://doi.org/10.1186/1465-9921-7-96

Bibtex

@article{cf6f5f92c1d84a2194162f121a6576de,
title = "Spinal afferent neurons projecting to the rat lung and pleura express acid sensitive channels",
abstract = "BACKGROUND: The acid sensitive ion channels TRPV1 (transient receptor potential vanilloid receptor-1) and ASIC3 (acid sensing ion channel-3) respond to tissue acidification in the range that occurs during painful conditions such as inflammation and ischemia. Here, we investigated to which extent they are expressed by rat dorsal root ganglion neurons projecting to lung and pleura, respectively.METHODS: The tracer DiI was either injected into the left lung or applied to the costal pleura. Retrogradely labelled dorsal root ganglion neurons were subjected to triple-labelling immunohistochemistry using antisera against TRPV1, ASIC3 and neurofilament 68 (marker for myelinated neurons), and their soma diameter was measured.RESULTS: Whereas 22% of pulmonary spinal afferents contained neither channel-immunoreactivity, at least one is expressed by 97% of pleural afferents. TRPV1+/ASIC3- neurons with probably slow conduction velocity (small soma, neurofilament 68-negative) were significantly more frequent among pleural (35%) than pulmonary afferents (20%). TRPV1+/ASIC3+ neurons amounted to 14 and 10% respectively. TRPV1-/ASIC3+ neurons made up between 44% (lung) and 48% (pleura) of neurons, and half of them presumably conducted in the A-fibre range (larger soma, neurofilament 68-positive).CONCLUSION: Rat pleural and pulmonary spinal afferents express at least two different acid-sensitive channels that make them suitable to monitor tissue acidification. Patterns of co-expression and structural markers define neuronal subgroups that can be inferred to subserve different functions and may initiate specific reflex responses. The higher prevalence of TRPV1+/ASIC3- neurons among pleural afferents probably reflects the high sensitivity of the parietal pleura to painful stimuli.",
keywords = "Acid Sensing Ion Channels, Animals, Carbocyanines, Cell Size, Female, Fluorescent Dyes, Ganglia, Spinal, Immunohistochemistry, Lung, Membrane Proteins, Nerve Tissue Proteins, Neurofilament Proteins, Neurons, Afferent, Pleura, Rats, Rats, Wistar, Sodium Channels, TRPV Cation Channels",
author = "Michael Groth and Tanja Helbig and Veronika Grau and Wolfgang Kummer and Haberberger, {Rainer V}",
year = "2006",
doi = "10.1186/1465-9921-7-96",
language = "English",
volume = "7",
pages = "96",
journal = "RESP RES",
issn = "1465-993X",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - Spinal afferent neurons projecting to the rat lung and pleura express acid sensitive channels

AU - Groth, Michael

AU - Helbig, Tanja

AU - Grau, Veronika

AU - Kummer, Wolfgang

AU - Haberberger, Rainer V

PY - 2006

Y1 - 2006

N2 - BACKGROUND: The acid sensitive ion channels TRPV1 (transient receptor potential vanilloid receptor-1) and ASIC3 (acid sensing ion channel-3) respond to tissue acidification in the range that occurs during painful conditions such as inflammation and ischemia. Here, we investigated to which extent they are expressed by rat dorsal root ganglion neurons projecting to lung and pleura, respectively.METHODS: The tracer DiI was either injected into the left lung or applied to the costal pleura. Retrogradely labelled dorsal root ganglion neurons were subjected to triple-labelling immunohistochemistry using antisera against TRPV1, ASIC3 and neurofilament 68 (marker for myelinated neurons), and their soma diameter was measured.RESULTS: Whereas 22% of pulmonary spinal afferents contained neither channel-immunoreactivity, at least one is expressed by 97% of pleural afferents. TRPV1+/ASIC3- neurons with probably slow conduction velocity (small soma, neurofilament 68-negative) were significantly more frequent among pleural (35%) than pulmonary afferents (20%). TRPV1+/ASIC3+ neurons amounted to 14 and 10% respectively. TRPV1-/ASIC3+ neurons made up between 44% (lung) and 48% (pleura) of neurons, and half of them presumably conducted in the A-fibre range (larger soma, neurofilament 68-positive).CONCLUSION: Rat pleural and pulmonary spinal afferents express at least two different acid-sensitive channels that make them suitable to monitor tissue acidification. Patterns of co-expression and structural markers define neuronal subgroups that can be inferred to subserve different functions and may initiate specific reflex responses. The higher prevalence of TRPV1+/ASIC3- neurons among pleural afferents probably reflects the high sensitivity of the parietal pleura to painful stimuli.

AB - BACKGROUND: The acid sensitive ion channels TRPV1 (transient receptor potential vanilloid receptor-1) and ASIC3 (acid sensing ion channel-3) respond to tissue acidification in the range that occurs during painful conditions such as inflammation and ischemia. Here, we investigated to which extent they are expressed by rat dorsal root ganglion neurons projecting to lung and pleura, respectively.METHODS: The tracer DiI was either injected into the left lung or applied to the costal pleura. Retrogradely labelled dorsal root ganglion neurons were subjected to triple-labelling immunohistochemistry using antisera against TRPV1, ASIC3 and neurofilament 68 (marker for myelinated neurons), and their soma diameter was measured.RESULTS: Whereas 22% of pulmonary spinal afferents contained neither channel-immunoreactivity, at least one is expressed by 97% of pleural afferents. TRPV1+/ASIC3- neurons with probably slow conduction velocity (small soma, neurofilament 68-negative) were significantly more frequent among pleural (35%) than pulmonary afferents (20%). TRPV1+/ASIC3+ neurons amounted to 14 and 10% respectively. TRPV1-/ASIC3+ neurons made up between 44% (lung) and 48% (pleura) of neurons, and half of them presumably conducted in the A-fibre range (larger soma, neurofilament 68-positive).CONCLUSION: Rat pleural and pulmonary spinal afferents express at least two different acid-sensitive channels that make them suitable to monitor tissue acidification. Patterns of co-expression and structural markers define neuronal subgroups that can be inferred to subserve different functions and may initiate specific reflex responses. The higher prevalence of TRPV1+/ASIC3- neurons among pleural afferents probably reflects the high sensitivity of the parietal pleura to painful stimuli.

KW - Acid Sensing Ion Channels

KW - Animals

KW - Carbocyanines

KW - Cell Size

KW - Female

KW - Fluorescent Dyes

KW - Ganglia, Spinal

KW - Immunohistochemistry

KW - Lung

KW - Membrane Proteins

KW - Nerve Tissue Proteins

KW - Neurofilament Proteins

KW - Neurons, Afferent

KW - Pleura

KW - Rats

KW - Rats, Wistar

KW - Sodium Channels

KW - TRPV Cation Channels

U2 - 10.1186/1465-9921-7-96

DO - 10.1186/1465-9921-7-96

M3 - SCORING: Journal article

C2 - 16813657

VL - 7

SP - 96

JO - RESP RES

JF - RESP RES

SN - 1465-993X

ER -