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Specificity protein 2 (Sp2) is essential for mouse development and autonomous proliferation of mouse embryonic fibroblasts

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Specificity protein 2 (Sp2) is essential for mouse development and autonomous proliferation of mouse embryonic fibroblasts. / Baur, Frank; Nau, Kerstin; Sadic, Dennis; Allweiss, Lena; Elsässer, Hans-Peter; Gillemans, Nynke; de Wit, Ton; Krüger, Imme; Vollmer, Marion; Philipsen, Sjaak; Suske, Guntram.

In: PLOS ONE, Vol. 5, No. 3, 01.01.2010, p. e9587.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Baur, F, Nau, K, Sadic, D, Allweiss, L, Elsässer, H-P, Gillemans, N, de Wit, T, Krüger, I, Vollmer, M, Philipsen, S & Suske, G 2010, 'Specificity protein 2 (Sp2) is essential for mouse development and autonomous proliferation of mouse embryonic fibroblasts', PLOS ONE, vol. 5, no. 3, pp. e9587. https://doi.org/10.1371/journal.pone.0009587

APA

Baur, F., Nau, K., Sadic, D., Allweiss, L., Elsässer, H-P., Gillemans, N., de Wit, T., Krüger, I., Vollmer, M., Philipsen, S., & Suske, G. (2010). Specificity protein 2 (Sp2) is essential for mouse development and autonomous proliferation of mouse embryonic fibroblasts. PLOS ONE, 5(3), e9587. https://doi.org/10.1371/journal.pone.0009587

Vancouver

Baur F, Nau K, Sadic D, Allweiss L, Elsässer H-P, Gillemans N et al. Specificity protein 2 (Sp2) is essential for mouse development and autonomous proliferation of mouse embryonic fibroblasts. PLOS ONE. 2010 Jan 1;5(3):e9587. https://doi.org/10.1371/journal.pone.0009587

Bibtex

@article{05af7f0a8c6e4b3f9717b37637e0070a,
title = "Specificity protein 2 (Sp2) is essential for mouse development and autonomous proliferation of mouse embryonic fibroblasts",
abstract = "BACKGROUND: The zinc finger protein Sp2 (specificity protein 2) is a member of the glutamine-rich Sp family of transcription factors. Despite its close similarity to Sp1, Sp3 and Sp4, Sp2 does not bind to DNA or activate transcription when expressed in mammalian cell lines. The expression pattern and the biological relevance of Sp2 in the mouse are unknown.METHODOLOGY/PRINCIPAL FINDINGS: Whole-mount in situ hybridization of mouse embryos between E7.5 and E9.5 revealed abundant expression in most embryonic and extra-embryonic tissues. In order to unravel the biological relevance of Sp2, we have targeted the Sp2 gene by a tri-loxP strategy. Constitutive Sp2null and conditional Sp2cko knockout alleles were obtained by crossings with appropriate Cre recombinase expressing mice. Constitutive disruption of the mouse Sp2 gene (Sp2null) resulted in severe growth retardation and lethality before E9.5. Mouse embryonic fibroblasts (MEFs) derived from Sp2null embryos at E9.5 failed to grow. Cre-mediated ablation of Sp2 in Sp2cko/cko MEFs obtained from E13.5 strongly impaired cell proliferation.CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that Sp2 is essential for early mouse development and autonomous proliferation of MEFs in culture. Comparison of the Sp2 knockout phenotype with the phenotypes of Sp1, Sp3 and Sp4 knockout strains shows that, despite their structural similarity and evolutionary relationship, all four glutamine-rich members of the Sp family of transcription factors have distinct non-redundant functions in vivo.",
keywords = "Animals, Cell Proliferation, DNA, Evolution, Molecular, Female, Fibroblasts, Gene Expression Regulation, Developmental, In Situ Hybridization, Male, Mice, Mice, Knockout, Phenotype, Sp2 Transcription Factor, Time Factors, Transcription, Genetic",
author = "Frank Baur and Kerstin Nau and Dennis Sadic and Lena Allweiss and Hans-Peter Els{\"a}sser and Nynke Gillemans and {de Wit}, Ton and Imme Kr{\"u}ger and Marion Vollmer and Sjaak Philipsen and Guntram Suske",
year = "2010",
month = jan,
day = "1",
doi = "10.1371/journal.pone.0009587",
language = "English",
volume = "5",
pages = "e9587",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

RIS

TY - JOUR

T1 - Specificity protein 2 (Sp2) is essential for mouse development and autonomous proliferation of mouse embryonic fibroblasts

AU - Baur, Frank

AU - Nau, Kerstin

AU - Sadic, Dennis

AU - Allweiss, Lena

AU - Elsässer, Hans-Peter

AU - Gillemans, Nynke

AU - de Wit, Ton

AU - Krüger, Imme

AU - Vollmer, Marion

AU - Philipsen, Sjaak

AU - Suske, Guntram

PY - 2010/1/1

Y1 - 2010/1/1

N2 - BACKGROUND: The zinc finger protein Sp2 (specificity protein 2) is a member of the glutamine-rich Sp family of transcription factors. Despite its close similarity to Sp1, Sp3 and Sp4, Sp2 does not bind to DNA or activate transcription when expressed in mammalian cell lines. The expression pattern and the biological relevance of Sp2 in the mouse are unknown.METHODOLOGY/PRINCIPAL FINDINGS: Whole-mount in situ hybridization of mouse embryos between E7.5 and E9.5 revealed abundant expression in most embryonic and extra-embryonic tissues. In order to unravel the biological relevance of Sp2, we have targeted the Sp2 gene by a tri-loxP strategy. Constitutive Sp2null and conditional Sp2cko knockout alleles were obtained by crossings with appropriate Cre recombinase expressing mice. Constitutive disruption of the mouse Sp2 gene (Sp2null) resulted in severe growth retardation and lethality before E9.5. Mouse embryonic fibroblasts (MEFs) derived from Sp2null embryos at E9.5 failed to grow. Cre-mediated ablation of Sp2 in Sp2cko/cko MEFs obtained from E13.5 strongly impaired cell proliferation.CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that Sp2 is essential for early mouse development and autonomous proliferation of MEFs in culture. Comparison of the Sp2 knockout phenotype with the phenotypes of Sp1, Sp3 and Sp4 knockout strains shows that, despite their structural similarity and evolutionary relationship, all four glutamine-rich members of the Sp family of transcription factors have distinct non-redundant functions in vivo.

AB - BACKGROUND: The zinc finger protein Sp2 (specificity protein 2) is a member of the glutamine-rich Sp family of transcription factors. Despite its close similarity to Sp1, Sp3 and Sp4, Sp2 does not bind to DNA or activate transcription when expressed in mammalian cell lines. The expression pattern and the biological relevance of Sp2 in the mouse are unknown.METHODOLOGY/PRINCIPAL FINDINGS: Whole-mount in situ hybridization of mouse embryos between E7.5 and E9.5 revealed abundant expression in most embryonic and extra-embryonic tissues. In order to unravel the biological relevance of Sp2, we have targeted the Sp2 gene by a tri-loxP strategy. Constitutive Sp2null and conditional Sp2cko knockout alleles were obtained by crossings with appropriate Cre recombinase expressing mice. Constitutive disruption of the mouse Sp2 gene (Sp2null) resulted in severe growth retardation and lethality before E9.5. Mouse embryonic fibroblasts (MEFs) derived from Sp2null embryos at E9.5 failed to grow. Cre-mediated ablation of Sp2 in Sp2cko/cko MEFs obtained from E13.5 strongly impaired cell proliferation.CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that Sp2 is essential for early mouse development and autonomous proliferation of MEFs in culture. Comparison of the Sp2 knockout phenotype with the phenotypes of Sp1, Sp3 and Sp4 knockout strains shows that, despite their structural similarity and evolutionary relationship, all four glutamine-rich members of the Sp family of transcription factors have distinct non-redundant functions in vivo.

KW - Animals

KW - Cell Proliferation

KW - DNA

KW - Evolution, Molecular

KW - Female

KW - Fibroblasts

KW - Gene Expression Regulation, Developmental

KW - In Situ Hybridization

KW - Male

KW - Mice

KW - Mice, Knockout

KW - Phenotype

KW - Sp2 Transcription Factor

KW - Time Factors

KW - Transcription, Genetic

U2 - 10.1371/journal.pone.0009587

DO - 10.1371/journal.pone.0009587

M3 - SCORING: Journal article

C2 - 20221402

VL - 5

SP - e9587

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 3

ER -