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Specification of germ cell fate in mice

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Specification of germ cell fate in mice. / Saitou, Mitinori; Payer, Bernhard; Lange, Ulrike C; Erhardt, Sylvia; Barton, Sheila C; Surani, M Azim.

In: PHILOS T R SOC B, Vol. 358, No. 1436, 29.08.2003, p. 1363-70.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Saitou, M, Payer, B, Lange, UC, Erhardt, S, Barton, SC & Surani, MA 2003, 'Specification of germ cell fate in mice', PHILOS T R SOC B, vol. 358, no. 1436, pp. 1363-70. https://doi.org/10.1098/rstb.2003.1324

APA

Saitou, M., Payer, B., Lange, U. C., Erhardt, S., Barton, S. C., & Surani, M. A. (2003). Specification of germ cell fate in mice. PHILOS T R SOC B, 358(1436), 1363-70. https://doi.org/10.1098/rstb.2003.1324

Vancouver

Saitou M, Payer B, Lange UC, Erhardt S, Barton SC, Surani MA. Specification of germ cell fate in mice. PHILOS T R SOC B. 2003 Aug 29;358(1436):1363-70. https://doi.org/10.1098/rstb.2003.1324

Bibtex

@article{a1667fcfc425434fa3f1b087488ba667,
title = "Specification of germ cell fate in mice",
abstract = "An early fundamental event during development is the segregation of germ cells from somatic cells. In many organisms, this is accomplished by the inheritance of preformed germ plasm, which apparently imposes transcriptional repression to prevent somatic cell fate. However, in mammals, pluripotent epiblast cells acquire germ cell fate in response to signalling molecules. We have used single cell analysis to study how epiblast cells acquire germ cell competence and undergo specification. Germ cell competent cells express Fragilis and initially progress towards a somatic mesodermal fate. However, a subset of these cells, the future primordial germ cells (PGCs), then shows rapid upregulation of Fragilis with concomitant transcriptional repression of a number of genes, including Hox and Smad genes. This repression may be a key event associated with germ cell specification. Furthermore, PGCs express Stella and other genes, such as Oct-4 that are associated with pluripotency. While these molecules are also detected in mature oocytes as maternally inherited factors, their early role is to regulate development and maintain pluripotency, and they do not serve the role of classical germline determinants.",
keywords = "Animals, Cell Differentiation, Gene Expression Regulation, Developmental, Gene Silencing, Germ Cells, Membrane Proteins, Mice, Pluripotent Stem Cells, Signal Transduction, Transcription, Genetic, Up-Regulation",
author = "Mitinori Saitou and Bernhard Payer and Lange, {Ulrike C} and Sylvia Erhardt and Barton, {Sheila C} and Surani, {M Azim}",
year = "2003",
month = aug,
day = "29",
doi = "10.1098/rstb.2003.1324",
language = "English",
volume = "358",
pages = "1363--70",
journal = "PHILOS T R SOC B",
issn = "0962-8436",
publisher = "Royal Society of London",
number = "1436",

}

RIS

TY - JOUR

T1 - Specification of germ cell fate in mice

AU - Saitou, Mitinori

AU - Payer, Bernhard

AU - Lange, Ulrike C

AU - Erhardt, Sylvia

AU - Barton, Sheila C

AU - Surani, M Azim

PY - 2003/8/29

Y1 - 2003/8/29

N2 - An early fundamental event during development is the segregation of germ cells from somatic cells. In many organisms, this is accomplished by the inheritance of preformed germ plasm, which apparently imposes transcriptional repression to prevent somatic cell fate. However, in mammals, pluripotent epiblast cells acquire germ cell fate in response to signalling molecules. We have used single cell analysis to study how epiblast cells acquire germ cell competence and undergo specification. Germ cell competent cells express Fragilis and initially progress towards a somatic mesodermal fate. However, a subset of these cells, the future primordial germ cells (PGCs), then shows rapid upregulation of Fragilis with concomitant transcriptional repression of a number of genes, including Hox and Smad genes. This repression may be a key event associated with germ cell specification. Furthermore, PGCs express Stella and other genes, such as Oct-4 that are associated with pluripotency. While these molecules are also detected in mature oocytes as maternally inherited factors, their early role is to regulate development and maintain pluripotency, and they do not serve the role of classical germline determinants.

AB - An early fundamental event during development is the segregation of germ cells from somatic cells. In many organisms, this is accomplished by the inheritance of preformed germ plasm, which apparently imposes transcriptional repression to prevent somatic cell fate. However, in mammals, pluripotent epiblast cells acquire germ cell fate in response to signalling molecules. We have used single cell analysis to study how epiblast cells acquire germ cell competence and undergo specification. Germ cell competent cells express Fragilis and initially progress towards a somatic mesodermal fate. However, a subset of these cells, the future primordial germ cells (PGCs), then shows rapid upregulation of Fragilis with concomitant transcriptional repression of a number of genes, including Hox and Smad genes. This repression may be a key event associated with germ cell specification. Furthermore, PGCs express Stella and other genes, such as Oct-4 that are associated with pluripotency. While these molecules are also detected in mature oocytes as maternally inherited factors, their early role is to regulate development and maintain pluripotency, and they do not serve the role of classical germline determinants.

KW - Animals

KW - Cell Differentiation

KW - Gene Expression Regulation, Developmental

KW - Gene Silencing

KW - Germ Cells

KW - Membrane Proteins

KW - Mice

KW - Pluripotent Stem Cells

KW - Signal Transduction

KW - Transcription, Genetic

KW - Up-Regulation

U2 - 10.1098/rstb.2003.1324

DO - 10.1098/rstb.2003.1324

M3 - SCORING: Journal article

C2 - 14511483

VL - 358

SP - 1363

EP - 1370

JO - PHILOS T R SOC B

JF - PHILOS T R SOC B

SN - 0962-8436

IS - 1436

ER -