Somatostatin regulates tight junction function and composition in human keratinocytes.
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Somatostatin regulates tight junction function and composition in human keratinocytes. / Vockel, Matthias; Breitenbach, Ute; Kreienkamp, Hans-Jürgen; Brandner, Johanna M.
In: EXP DERMATOL, Vol. 19, No. 10, 10, 2010, p. 888-894.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Somatostatin regulates tight junction function and composition in human keratinocytes.
AU - Vockel, Matthias
AU - Breitenbach, Ute
AU - Kreienkamp, Hans-Jürgen
AU - Brandner, Johanna M
PY - 2010
Y1 - 2010
N2 - Somatostatin (SST) is a regulatory peptide hormone that acts through five different G protein-coupled receptors (SSTR1-5). Whereas expression of all five SSTR subtypes in epidermis has been shown, the biological relevance of the SST/SSTR system in the skin is completely unknown. We show here that SST is expressed in human skin and is present in a subset of Merkel cells and dendritic cells as well as in keratinocytes. We focused further on the somatostatin receptor subtype 3 (SSTR3) and its interacting protein MUPP1, as both were found to be localized at cellular junctions in epidermal keratinocytes. MUPP1 is a component of tight junctions (TJs); these cell-cell junctions contribute to barrier function of the paracellular pathway in cultured keratinocytes. We provide evidence that SSTR3 and MUPP1 interact in primary cultured human keratinocytes at high Ca(2+) conditions. Interestingly, SST, presumably via SSTR3/MUPP1, regulates TJ permeability in cultured keratinocytes. During long-term treatment of human keratinocytes, SST also affects the expression of distinct TJ proteins such as claudin-4. Our data are the first example of a peptide hormone regulating TJ functionality and composition in human keratinocytes, suggesting that control via peptide hormones provides the possibility to regulate the TJ barrier characteristics of the skin.
AB - Somatostatin (SST) is a regulatory peptide hormone that acts through five different G protein-coupled receptors (SSTR1-5). Whereas expression of all five SSTR subtypes in epidermis has been shown, the biological relevance of the SST/SSTR system in the skin is completely unknown. We show here that SST is expressed in human skin and is present in a subset of Merkel cells and dendritic cells as well as in keratinocytes. We focused further on the somatostatin receptor subtype 3 (SSTR3) and its interacting protein MUPP1, as both were found to be localized at cellular junctions in epidermal keratinocytes. MUPP1 is a component of tight junctions (TJs); these cell-cell junctions contribute to barrier function of the paracellular pathway in cultured keratinocytes. We provide evidence that SSTR3 and MUPP1 interact in primary cultured human keratinocytes at high Ca(2+) conditions. Interestingly, SST, presumably via SSTR3/MUPP1, regulates TJ permeability in cultured keratinocytes. During long-term treatment of human keratinocytes, SST also affects the expression of distinct TJ proteins such as claudin-4. Our data are the first example of a peptide hormone regulating TJ functionality and composition in human keratinocytes, suggesting that control via peptide hormones provides the possibility to regulate the TJ barrier characteristics of the skin.
KW - Adult
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Adolescent
KW - Young Adult
KW - Cells, Cultured
KW - RNA, Messenger metabolism
KW - Calcium metabolism
KW - Carrier Proteins genetics
KW - Claudins metabolism
KW - Cyclic AMP metabolism
KW - Keratinocytes cytology
KW - Permeability
KW - Receptors, Somatostatin genetics
KW - Somatostatin genetics
KW - Tight Junctions metabolism
KW - Adult
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Adolescent
KW - Young Adult
KW - Cells, Cultured
KW - RNA, Messenger metabolism
KW - Calcium metabolism
KW - Carrier Proteins genetics
KW - Claudins metabolism
KW - Cyclic AMP metabolism
KW - Keratinocytes cytology
KW - Permeability
KW - Receptors, Somatostatin genetics
KW - Somatostatin genetics
KW - Tight Junctions metabolism
M3 - SCORING: Zeitschriftenaufsatz
VL - 19
SP - 888
EP - 894
JO - EXP DERMATOL
JF - EXP DERMATOL
SN - 0906-6705
IS - 10
M1 - 10
ER -
