Single-Cell Electroporation of Neurons

J Simon Wiegert; Christine E Gee; Thomas G Oertner

doi:10.1101/pdb.prot094904

Single-Cell Electroporation of Neurons

Standard

Single-Cell Electroporation of Neurons. / Wiegert, J Simon; Gee, Christine E ; Oertner, Thomas G.

In: Cold Spring Harbor protocols, Vol. 2017, No. 2, 01.02.2017, p. pdb.prot094904.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wiegert, JS, Gee, CE & Oertner, TG 2017, 'Single-Cell Electroporation of Neurons', Cold Spring Harbor protocols, vol. 2017, no. 2, pp. pdb.prot094904. https://doi.org/10.1101/pdb.prot094904

APA

Wiegert, J. S., Gee, C. E., & Oertner, T. G. (2017). Single-Cell Electroporation of Neurons. Cold Spring Harbor protocols, 2017(2), pdb.prot094904. https://doi.org/10.1101/pdb.prot094904

Vancouver

Wiegert JS, Gee CE , Oertner TG. Single-Cell Electroporation of Neurons. Cold Spring Harbor protocols. 2017 Feb 1;2017(2):pdb.prot094904. https://doi.org/10.1101/pdb.prot094904

Bibtex

@article{f442325e0f19412ca8f08a9cd3fb4777,

title = "Single-Cell Electroporation of Neurons",

abstract = "Single-cell electroporation allows the transfection of a small number of neurons in an organotypic culture with a single plasmid or a defined mixture of plasmids. Desired protein expression levels can vary depending on the experimental goals (e.g., high expression levels are needed for optogenetic experiments); however, when too much protein is expressed, cellular toxicity and cell death may arise. To a large degree, protein expression can be controlled by adjusting the concentration of plasmid DNA in the electroporation pipette. Here, we present a protocol for transfecting individual neurons in hippocampal slice cultures by electroporation. Essentially, a patch-clamp setup is required that includes an upright microscope with infrared differential interference contrast or Dodt contrast with a camera and a specialized amplifier that is able to deliver large-voltage pulses to the electroporation pipette.",

author = "Wiegert, {J Simon} and Gee, {Christine E} and Oertner, {Thomas G}",

note = "{\textcopyright} 2017 Cold Spring Harbor Laboratory Press.",

year = "2017",

month = feb,

day = "1",

doi = "10.1101/pdb.prot094904",

language = "English",

volume = "2017",

pages = "pdb.prot094904",

journal = "Cold Spring Harbor protocols",

issn = "1559-6095",

publisher = "Cold Spring Harbor Laboratory Press",

number = "2",

}

RIS

TY - JOUR

T1 - Single-Cell Electroporation of Neurons

AU - Wiegert, J Simon

AU - Gee, Christine E

AU - Oertner, Thomas G

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Single-cell electroporation allows the transfection of a small number of neurons in an organotypic culture with a single plasmid or a defined mixture of plasmids. Desired protein expression levels can vary depending on the experimental goals (e.g., high expression levels are needed for optogenetic experiments); however, when too much protein is expressed, cellular toxicity and cell death may arise. To a large degree, protein expression can be controlled by adjusting the concentration of plasmid DNA in the electroporation pipette. Here, we present a protocol for transfecting individual neurons in hippocampal slice cultures by electroporation. Essentially, a patch-clamp setup is required that includes an upright microscope with infrared differential interference contrast or Dodt contrast with a camera and a specialized amplifier that is able to deliver large-voltage pulses to the electroporation pipette.

AB - Single-cell electroporation allows the transfection of a small number of neurons in an organotypic culture with a single plasmid or a defined mixture of plasmids. Desired protein expression levels can vary depending on the experimental goals (e.g., high expression levels are needed for optogenetic experiments); however, when too much protein is expressed, cellular toxicity and cell death may arise. To a large degree, protein expression can be controlled by adjusting the concentration of plasmid DNA in the electroporation pipette. Here, we present a protocol for transfecting individual neurons in hippocampal slice cultures by electroporation. Essentially, a patch-clamp setup is required that includes an upright microscope with infrared differential interference contrast or Dodt contrast with a camera and a specialized amplifier that is able to deliver large-voltage pulses to the electroporation pipette.

U2 - 10.1101/pdb.prot094904

DO - 10.1101/pdb.prot094904

M3 - SCORING: Journal article

C2 - 28148856

VL - 2017

SP - pdb.prot094904

JO - Cold Spring Harbor protocols

JF - Cold Spring Harbor protocols

SN - 1559-6095

IS - 2

ER -