Single-cell adhesion of human osteoblasts on plasma-conditioned titanium implant surfaces in vitro
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Single-cell adhesion of human osteoblasts on plasma-conditioned titanium implant surfaces in vitro. / Becker, Merlind; Schmied, Frederike; Kadem, Laith F; Freitag-Wolf, Sandra; Naujokat, Hendrik; Mehl, Christian; Kern, Matthias; Harder, Sönke.
In: J MECH BEHAV BIOMED, Vol. 109, 09.2020, p. 103841.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Single-cell adhesion of human osteoblasts on plasma-conditioned titanium implant surfaces in vitro
AU - Becker, Merlind
AU - Schmied, Frederike
AU - Kadem, Laith F
AU - Freitag-Wolf, Sandra
AU - Naujokat, Hendrik
AU - Mehl, Christian
AU - Kern, Matthias
AU - Harder, Sönke
N1 - Copyright © 2020 Elsevier Ltd. All rights reserved.
PY - 2020/9
Y1 - 2020/9
N2 - OBJECTIVES: This study aimed to demonstrate the effect of treating titanium-implant surfaces with plasma from two different sources on wettability and initial single-cell adhesion of human osteoblasts and to investigate whether aging affects treatment outcomes.METHODS: Titanium disks with sandblasted and acid-etched (SLA) surfaces were treated with atmospheric pressure plasma (APP) and low-pressure plasma (LPP). For wetting behavior of the specimens after plasma treatment, the water contact angle was measured. The single-cell detachment force and amount of work of detachment of human osteoblasts were determined with single-cell force spectroscopy (SCFS). To evaluate the aging effect in APP-treated specimens, SCFS was conducted 10 and 60 min after treatment.RESULTS: Significantly higher hydrophilicity was observed in the APP and LPP treatment groups than in the control group, but no significant difference was observed between the APP and LPP groups. No significant difference in cell-detachment force or work of detachment was observed, and there were no significant differences according to the conditioning mechanisms and storage time.SIGNIFICANCE: Conditioning of the titanium surfaces with APP or LPP was not a significant influencing factor in the initial adhesion of the osteoblasts.
AB - OBJECTIVES: This study aimed to demonstrate the effect of treating titanium-implant surfaces with plasma from two different sources on wettability and initial single-cell adhesion of human osteoblasts and to investigate whether aging affects treatment outcomes.METHODS: Titanium disks with sandblasted and acid-etched (SLA) surfaces were treated with atmospheric pressure plasma (APP) and low-pressure plasma (LPP). For wetting behavior of the specimens after plasma treatment, the water contact angle was measured. The single-cell detachment force and amount of work of detachment of human osteoblasts were determined with single-cell force spectroscopy (SCFS). To evaluate the aging effect in APP-treated specimens, SCFS was conducted 10 and 60 min after treatment.RESULTS: Significantly higher hydrophilicity was observed in the APP and LPP treatment groups than in the control group, but no significant difference was observed between the APP and LPP groups. No significant difference in cell-detachment force or work of detachment was observed, and there were no significant differences according to the conditioning mechanisms and storage time.SIGNIFICANCE: Conditioning of the titanium surfaces with APP or LPP was not a significant influencing factor in the initial adhesion of the osteoblasts.
KW - Cell Adhesion
KW - Dental Implants
KW - Humans
KW - Osteoblasts
KW - Surface Properties
KW - Titanium
KW - Wettability
U2 - 10.1016/j.jmbbm.2020.103841
DO - 10.1016/j.jmbbm.2020.103841
M3 - SCORING: Journal article
C2 - 32543406
VL - 109
SP - 103841
JO - J MECH BEHAV BIOMED
JF - J MECH BEHAV BIOMED
SN - 1751-6161
ER -
