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Silver nanoparticle-enriched diamond-like carbon implant modification as a mammalian cell compatible surface with antimicrobial properties

Standard

Silver nanoparticle-enriched diamond-like carbon implant modification as a mammalian cell compatible surface with antimicrobial properties. / Gorzelanny, Christian; Kmeth, Ralf; Obermeier, Andreas; Bauer, Alexander T; Halter, Natalia; Kümpel, Katharina; Schneider, Matthias F; Wixforth, Achim; Gollwitzer, Hans; Burgkart, Rainer; Stritzker, Bernd; Schneider, Stefan W.

In: SCI REP-UK, Vol. 6, 09.03.2016, p. 22849.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Gorzelanny, C, Kmeth, R, Obermeier, A, Bauer, AT, Halter, N, Kümpel, K, Schneider, MF, Wixforth, A, Gollwitzer, H, Burgkart, R, Stritzker, B & Schneider, SW 2016, 'Silver nanoparticle-enriched diamond-like carbon implant modification as a mammalian cell compatible surface with antimicrobial properties', SCI REP-UK, vol. 6, pp. 22849. https://doi.org/10.1038/srep22849

APA

Gorzelanny, C., Kmeth, R., Obermeier, A., Bauer, A. T., Halter, N., Kümpel, K., Schneider, M. F., Wixforth, A., Gollwitzer, H., Burgkart, R., Stritzker, B., & Schneider, S. W. (2016). Silver nanoparticle-enriched diamond-like carbon implant modification as a mammalian cell compatible surface with antimicrobial properties. SCI REP-UK, 6, 22849. https://doi.org/10.1038/srep22849

Vancouver

Gorzelanny C, Kmeth R, Obermeier A, Bauer AT, Halter N, Kümpel K et al. Silver nanoparticle-enriched diamond-like carbon implant modification as a mammalian cell compatible surface with antimicrobial properties. SCI REP-UK. 2016 Mar 9;6:22849. https://doi.org/10.1038/srep22849

Bibtex

@article{92cfcd504d9f402c88a46879eb592fd4,
title = "Silver nanoparticle-enriched diamond-like carbon implant modification as a mammalian cell compatible surface with antimicrobial properties",
abstract = "The implant-bone interface is the scene of competition between microorganisms and distinct types of tissue cells. In the past, various strategies have been followed to support bony integration and to prevent bacterial implant-associated infections. In the present study we investigated the biological properties of diamond-like carbon (DLC) surfaces containing silver nanoparticles. DLC is a promising material for the modification of medical implants providing high mechanical and chemical stability and a high degree of biocompatibility. DLC surface modifications with varying silver concentrations were generated on medical-grade titanium discs, using plasma immersion ion implantation-induced densification of silver nanoparticle-containing polyvinylpyrrolidone polymer solutions. Immersion of implants in aqueous liquids resulted in a rapid silver release reducing the growth of surface-bound and planktonic Staphylococcus aureus and Staphylococcus epidermidis. Due to the fast and transient release of silver ions from the modified implants, the surfaces became biocompatible, ensuring growth of mammalian cells. Human endothelial cells retained their cellular differentiation as indicated by the intracellular formation of Weibel-Palade bodies and a high responsiveness towards histamine. Our findings indicate that the integration of silver nanoparticles into DLC prevents bacterial colonization due to a fast initial release of silver ions, facilitating the growth of silver susceptible mammalian cells subsequently.",
keywords = "Anti-Bacterial Agents, Biocompatible Materials, Cell Differentiation, Diamond, Endothelial Cells, Humans, Nanoparticles, Prostheses and Implants, Silver, Staphylococcus aureus, Staphylococcus epidermidis, Surface Properties, Journal Article, Research Support, Non-U.S. Gov't",
author = "Christian Gorzelanny and Ralf Kmeth and Andreas Obermeier and Bauer, {Alexander T} and Natalia Halter and Katharina K{\"u}mpel and Schneider, {Matthias F} and Achim Wixforth and Hans Gollwitzer and Rainer Burgkart and Bernd Stritzker and Schneider, {Stefan W}",
year = "2016",
month = mar,
day = "9",
doi = "10.1038/srep22849",
language = "English",
volume = "6",
pages = "22849",
journal = "SCI REP-UK",
issn = "2045-2322",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - Silver nanoparticle-enriched diamond-like carbon implant modification as a mammalian cell compatible surface with antimicrobial properties

AU - Gorzelanny, Christian

AU - Kmeth, Ralf

AU - Obermeier, Andreas

AU - Bauer, Alexander T

AU - Halter, Natalia

AU - Kümpel, Katharina

AU - Schneider, Matthias F

AU - Wixforth, Achim

AU - Gollwitzer, Hans

AU - Burgkart, Rainer

AU - Stritzker, Bernd

AU - Schneider, Stefan W

PY - 2016/3/9

Y1 - 2016/3/9

N2 - The implant-bone interface is the scene of competition between microorganisms and distinct types of tissue cells. In the past, various strategies have been followed to support bony integration and to prevent bacterial implant-associated infections. In the present study we investigated the biological properties of diamond-like carbon (DLC) surfaces containing silver nanoparticles. DLC is a promising material for the modification of medical implants providing high mechanical and chemical stability and a high degree of biocompatibility. DLC surface modifications with varying silver concentrations were generated on medical-grade titanium discs, using plasma immersion ion implantation-induced densification of silver nanoparticle-containing polyvinylpyrrolidone polymer solutions. Immersion of implants in aqueous liquids resulted in a rapid silver release reducing the growth of surface-bound and planktonic Staphylococcus aureus and Staphylococcus epidermidis. Due to the fast and transient release of silver ions from the modified implants, the surfaces became biocompatible, ensuring growth of mammalian cells. Human endothelial cells retained their cellular differentiation as indicated by the intracellular formation of Weibel-Palade bodies and a high responsiveness towards histamine. Our findings indicate that the integration of silver nanoparticles into DLC prevents bacterial colonization due to a fast initial release of silver ions, facilitating the growth of silver susceptible mammalian cells subsequently.

AB - The implant-bone interface is the scene of competition between microorganisms and distinct types of tissue cells. In the past, various strategies have been followed to support bony integration and to prevent bacterial implant-associated infections. In the present study we investigated the biological properties of diamond-like carbon (DLC) surfaces containing silver nanoparticles. DLC is a promising material for the modification of medical implants providing high mechanical and chemical stability and a high degree of biocompatibility. DLC surface modifications with varying silver concentrations were generated on medical-grade titanium discs, using plasma immersion ion implantation-induced densification of silver nanoparticle-containing polyvinylpyrrolidone polymer solutions. Immersion of implants in aqueous liquids resulted in a rapid silver release reducing the growth of surface-bound and planktonic Staphylococcus aureus and Staphylococcus epidermidis. Due to the fast and transient release of silver ions from the modified implants, the surfaces became biocompatible, ensuring growth of mammalian cells. Human endothelial cells retained their cellular differentiation as indicated by the intracellular formation of Weibel-Palade bodies and a high responsiveness towards histamine. Our findings indicate that the integration of silver nanoparticles into DLC prevents bacterial colonization due to a fast initial release of silver ions, facilitating the growth of silver susceptible mammalian cells subsequently.

KW - Anti-Bacterial Agents

KW - Biocompatible Materials

KW - Cell Differentiation

KW - Diamond

KW - Endothelial Cells

KW - Humans

KW - Nanoparticles

KW - Prostheses and Implants

KW - Silver

KW - Staphylococcus aureus

KW - Staphylococcus epidermidis

KW - Surface Properties

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/srep22849

DO - 10.1038/srep22849

M3 - SCORING: Journal article

C2 - 26955791

VL - 6

SP - 22849

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

ER -