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Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment

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Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment. / Schulte-Schrepping, Jonas; Reusch, Nico; Paclik, Daniela; Germany, Life and Medical Sciences (LIMES) Institute University of; Schlickeiser, Stephan; Zhang, Bowen; Krämer, Benjamin; Krammer, Tobias; Brumhard, Sophia; Bonaguro, Lorenzo; De Domenico, Elena; Wendisch, Daniel; Grasshoff, Martin; Kapellos, Theodore S; Beckstette, Michael; Pecht, Tal; Saglam, Adem; Dietrich, Oliver; Mei, Henrik E; Schulz, Axel R; Conrad, Claudia; Kunkel, Désirée; Vafadarnejad, Ehsan; Xu, Cheng-Jian; Horne, Arik; Herbert, Miriam; Drews, Anna; Thibeault, Charlotte; Pfeiffer, Moritz; Hippenstiel, Stefan; Hocke, Andreas; Müller-Redetzky, Holger; Heim, Katrin-Moira; Machleidt, Felix; Uhrig, Alexander; Bosquillon de Jarcy, Laure; Jürgens, Linda; Stegemann, Miriam; Glösenkamp, Christoph R; Volk, Hans-Dieter; Goffinet, Christine; Landthaler, Markus; Wyler, Emanuel; Georg, Philipp; Schneider, Maria; Dang-Heine, Chantip; Neuwinger, Nick; Kappert, Kai; Tauber, Rudolf; Corman, Victor; Raabe, Jan; Kaiser, Kim Melanie; Vinh, Michael To; Rieke, Gereon; Meisel, Christian; Ulas, Thomas; Becker, Matthias; Geffers, Robert; Witzenrath, Martin; Drosten, Christian; Suttorp, Norbert; von Kalle, Christof; Kurth, Florian; Händler, Kristian; Schultze, Joachim L; Aschenbrenner, Anna C; Li, Yang; Nattermann, Jacob; Sawitzki, Birgit; Saliba, Antoine-Emmanuel; Sander, Leif Erik; Deutsche COVID-19 OMICS Initiative (DeCOI).

In: CELL, Vol. 182, No. 6, 17.09.2020, p. 1419-1440.e23.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schulte-Schrepping, J, Reusch, N, Paclik, D, Germany, LAMSIUO, Schlickeiser, S, Zhang, B, Krämer, B, Krammer, T, Brumhard, S, Bonaguro, L, De Domenico, E, Wendisch, D, Grasshoff, M, Kapellos, TS, Beckstette, M, Pecht, T, Saglam, A, Dietrich, O, Mei, HE, Schulz, AR, Conrad, C, Kunkel, D, Vafadarnejad, E, Xu, C-J, Horne, A, Herbert, M, Drews, A, Thibeault, C, Pfeiffer, M, Hippenstiel, S, Hocke, A, Müller-Redetzky, H, Heim, K-M, Machleidt, F, Uhrig, A, Bosquillon de Jarcy, L, Jürgens, L, Stegemann, M, Glösenkamp, CR, Volk, H-D, Goffinet, C, Landthaler, M, Wyler, E, Georg, P, Schneider, M, Dang-Heine, C, Neuwinger, N, Kappert, K, Tauber, R, Corman, V, Raabe, J, Kaiser, KM, Vinh, MT, Rieke, G, Meisel, C, Ulas, T, Becker, M, Geffers, R, Witzenrath, M, Drosten, C, Suttorp, N, von Kalle, C, Kurth, F, Händler, K, Schultze, JL, Aschenbrenner, AC, Li, Y, Nattermann, J, Sawitzki, B, Saliba, A-E, Sander, LE & Deutsche COVID-19 OMICS Initiative (DeCOI) 2020, 'Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment', CELL, vol. 182, no. 6, pp. 1419-1440.e23. https://doi.org/10.1016/j.cell.2020.08.001

APA

Schulte-Schrepping, J., Reusch, N., Paclik, D., Germany, L. A. M. S. I. U. O., Schlickeiser, S., Zhang, B., Krämer, B., Krammer, T., Brumhard, S., Bonaguro, L., De Domenico, E., Wendisch, D., Grasshoff, M., Kapellos, T. S., Beckstette, M., Pecht, T., Saglam, A., Dietrich, O., Mei, H. E., ... Deutsche COVID-19 OMICS Initiative (DeCOI) (2020). Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment. CELL, 182(6), 1419-1440.e23. https://doi.org/10.1016/j.cell.2020.08.001

Vancouver

Schulte-Schrepping J, Reusch N, Paclik D, Germany LAMSIUO, Schlickeiser S, Zhang B et al. Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment. CELL. 2020 Sep 17;182(6):1419-1440.e23. https://doi.org/10.1016/j.cell.2020.08.001

Bibtex

@article{f7f1d59a9fb54cdeb547b41744e41cb6,
title = "Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment",
abstract = "Coronavirus disease 2019 (COVID-19) is a mild to moderate respiratory tract infection, however, a subset of patients progress to severe disease and respiratory failure. The mechanism of protective immunity in mild forms and the pathogenesis of severe COVID-19 associated with increased neutrophil counts and dysregulated immune responses remain unclear. In a dual-center, two-cohort study, we combined single-cell RNA-sequencing and single-cell proteomics of whole-blood and peripheral-blood mononuclear cells to determine changes in immune cell composition and activation in mild versus severe COVID-19 (242 samples from 109 individuals) over time. HLA-DRhiCD11chi inflammatory monocytes with an interferon-stimulated gene signature were elevated in mild COVID-19. Severe COVID-19 was marked by occurrence of neutrophil precursors, as evidence of emergency myelopoiesis, dysfunctional mature neutrophils, and HLA-DRlo monocytes. Our study provides detailed insights into the systemic immune response to SARS-CoV-2 infection and reveals profound alterations in the myeloid cell compartment associated with severe COVID-19.",
keywords = "Adult, Aged, CD11 Antigens/genetics, COVID-19, Cells, Cultured, Coronavirus Infections/blood, Female, HLA-DR Antigens/genetics, Humans, Male, Middle Aged, Myeloid Cells/cytology, Myelopoiesis, Pandemics, Pneumonia, Viral/blood, Proteome/genetics, Proteomics, Single-Cell Analysis",
author = "Jonas Schulte-Schrepping and Nico Reusch and Daniela Paclik and Germany, {Life and Medical Sciences (LIMES) Institute University of} and Stephan Schlickeiser and Bowen Zhang and Benjamin Kr{\"a}mer and Tobias Krammer and Sophia Brumhard and Lorenzo Bonaguro and {De Domenico}, Elena and Daniel Wendisch and Martin Grasshoff and Kapellos, {Theodore S} and Michael Beckstette and Tal Pecht and Adem Saglam and Oliver Dietrich and Mei, {Henrik E} and Schulz, {Axel R} and Claudia Conrad and D{\'e}sir{\'e}e Kunkel and Ehsan Vafadarnejad and Cheng-Jian Xu and Arik Horne and Miriam Herbert and Anna Drews and Charlotte Thibeault and Moritz Pfeiffer and Stefan Hippenstiel and Andreas Hocke and Holger M{\"u}ller-Redetzky and Katrin-Moira Heim and Felix Machleidt and Alexander Uhrig and {Bosquillon de Jarcy}, Laure and Linda J{\"u}rgens and Miriam Stegemann and Gl{\"o}senkamp, {Christoph R} and Hans-Dieter Volk and Christine Goffinet and Markus Landthaler and Emanuel Wyler and Philipp Georg and Maria Schneider and Chantip Dang-Heine and Nick Neuwinger and Kai Kappert and Rudolf Tauber and Victor Corman and Jan Raabe and Kaiser, {Kim Melanie} and Vinh, {Michael To} and Gereon Rieke and Christian Meisel and Thomas Ulas and Matthias Becker and Robert Geffers and Martin Witzenrath and Christian Drosten and Norbert Suttorp and {von Kalle}, Christof and Florian Kurth and Kristian H{\"a}ndler and Schultze, {Joachim L} and Aschenbrenner, {Anna C} and Yang Li and Jacob Nattermann and Birgit Sawitzki and Antoine-Emmanuel Saliba and Sander, {Leif Erik} and {Deutsche COVID-19 OMICS Initiative (DeCOI)}",
note = "Copyright {\textcopyright} 2020 Elsevier Inc. All rights reserved.",
year = "2020",
month = sep,
day = "17",
doi = "10.1016/j.cell.2020.08.001",
language = "English",
volume = "182",
pages = "1419--1440.e23",
journal = "CELL",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",

}

RIS

TY - JOUR

T1 - Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment

AU - Schulte-Schrepping, Jonas

AU - Reusch, Nico

AU - Paclik, Daniela

AU - Germany, Life and Medical Sciences (LIMES) Institute University of

AU - Schlickeiser, Stephan

AU - Zhang, Bowen

AU - Krämer, Benjamin

AU - Krammer, Tobias

AU - Brumhard, Sophia

AU - Bonaguro, Lorenzo

AU - De Domenico, Elena

AU - Wendisch, Daniel

AU - Grasshoff, Martin

AU - Kapellos, Theodore S

AU - Beckstette, Michael

AU - Pecht, Tal

AU - Saglam, Adem

AU - Dietrich, Oliver

AU - Mei, Henrik E

AU - Schulz, Axel R

AU - Conrad, Claudia

AU - Kunkel, Désirée

AU - Vafadarnejad, Ehsan

AU - Xu, Cheng-Jian

AU - Horne, Arik

AU - Herbert, Miriam

AU - Drews, Anna

AU - Thibeault, Charlotte

AU - Pfeiffer, Moritz

AU - Hippenstiel, Stefan

AU - Hocke, Andreas

AU - Müller-Redetzky, Holger

AU - Heim, Katrin-Moira

AU - Machleidt, Felix

AU - Uhrig, Alexander

AU - Bosquillon de Jarcy, Laure

AU - Jürgens, Linda

AU - Stegemann, Miriam

AU - Glösenkamp, Christoph R

AU - Volk, Hans-Dieter

AU - Goffinet, Christine

AU - Landthaler, Markus

AU - Wyler, Emanuel

AU - Georg, Philipp

AU - Schneider, Maria

AU - Dang-Heine, Chantip

AU - Neuwinger, Nick

AU - Kappert, Kai

AU - Tauber, Rudolf

AU - Corman, Victor

AU - Raabe, Jan

AU - Kaiser, Kim Melanie

AU - Vinh, Michael To

AU - Rieke, Gereon

AU - Meisel, Christian

AU - Ulas, Thomas

AU - Becker, Matthias

AU - Geffers, Robert

AU - Witzenrath, Martin

AU - Drosten, Christian

AU - Suttorp, Norbert

AU - von Kalle, Christof

AU - Kurth, Florian

AU - Händler, Kristian

AU - Schultze, Joachim L

AU - Aschenbrenner, Anna C

AU - Li, Yang

AU - Nattermann, Jacob

AU - Sawitzki, Birgit

AU - Saliba, Antoine-Emmanuel

AU - Sander, Leif Erik

AU - Deutsche COVID-19 OMICS Initiative (DeCOI)

N1 - Copyright © 2020 Elsevier Inc. All rights reserved.

PY - 2020/9/17

Y1 - 2020/9/17

N2 - Coronavirus disease 2019 (COVID-19) is a mild to moderate respiratory tract infection, however, a subset of patients progress to severe disease and respiratory failure. The mechanism of protective immunity in mild forms and the pathogenesis of severe COVID-19 associated with increased neutrophil counts and dysregulated immune responses remain unclear. In a dual-center, two-cohort study, we combined single-cell RNA-sequencing and single-cell proteomics of whole-blood and peripheral-blood mononuclear cells to determine changes in immune cell composition and activation in mild versus severe COVID-19 (242 samples from 109 individuals) over time. HLA-DRhiCD11chi inflammatory monocytes with an interferon-stimulated gene signature were elevated in mild COVID-19. Severe COVID-19 was marked by occurrence of neutrophil precursors, as evidence of emergency myelopoiesis, dysfunctional mature neutrophils, and HLA-DRlo monocytes. Our study provides detailed insights into the systemic immune response to SARS-CoV-2 infection and reveals profound alterations in the myeloid cell compartment associated with severe COVID-19.

AB - Coronavirus disease 2019 (COVID-19) is a mild to moderate respiratory tract infection, however, a subset of patients progress to severe disease and respiratory failure. The mechanism of protective immunity in mild forms and the pathogenesis of severe COVID-19 associated with increased neutrophil counts and dysregulated immune responses remain unclear. In a dual-center, two-cohort study, we combined single-cell RNA-sequencing and single-cell proteomics of whole-blood and peripheral-blood mononuclear cells to determine changes in immune cell composition and activation in mild versus severe COVID-19 (242 samples from 109 individuals) over time. HLA-DRhiCD11chi inflammatory monocytes with an interferon-stimulated gene signature were elevated in mild COVID-19. Severe COVID-19 was marked by occurrence of neutrophil precursors, as evidence of emergency myelopoiesis, dysfunctional mature neutrophils, and HLA-DRlo monocytes. Our study provides detailed insights into the systemic immune response to SARS-CoV-2 infection and reveals profound alterations in the myeloid cell compartment associated with severe COVID-19.

KW - Adult

KW - Aged

KW - CD11 Antigens/genetics

KW - COVID-19

KW - Cells, Cultured

KW - Coronavirus Infections/blood

KW - Female

KW - HLA-DR Antigens/genetics

KW - Humans

KW - Male

KW - Middle Aged

KW - Myeloid Cells/cytology

KW - Myelopoiesis

KW - Pandemics

KW - Pneumonia, Viral/blood

KW - Proteome/genetics

KW - Proteomics

KW - Single-Cell Analysis

U2 - 10.1016/j.cell.2020.08.001

DO - 10.1016/j.cell.2020.08.001

M3 - SCORING: Journal article

C2 - 32810438

VL - 182

SP - 1419-1440.e23

JO - CELL

JF - CELL

SN - 0092-8674

IS - 6

ER -