Research ExplorerPublicationsSesquiterpene lactones inhibit Yersinia invasin protein-indu...

Sesquiterpene lactones inhibit Yersinia invasin protein-induced IL-8 and MCP-1 production in epithelial cells

Standard

Sesquiterpene lactones inhibit Yersinia invasin protein-induced IL-8 and MCP-1 production in epithelial cells. / Grassl, Guntram A; Fessele, Sabine; Merfort, Irmgard; Lindenmeyer, Maja T; Castro, Victor; Murillo, Renato; Nelson, Peter J; Autenrieth, Ingo B.

In: INT J MED MICROBIOL, Vol. 295, No. 8, 12.2005, p. 531-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Grassl, GA, Fessele, S, Merfort, I, Lindenmeyer, MT, Castro, V, Murillo, R, Nelson, PJ & Autenrieth, IB 2005, 'Sesquiterpene lactones inhibit Yersinia invasin protein-induced IL-8 and MCP-1 production in epithelial cells', INT J MED MICROBIOL, vol. 295, no. 8, pp. 531-8. https://doi.org/10.1016/j.ijmm.2005.07.012

APA

Grassl, G. A., Fessele, S., Merfort, I., Lindenmeyer, M. T., Castro, V., Murillo, R., Nelson, P. J., & Autenrieth, I. B. (2005). Sesquiterpene lactones inhibit Yersinia invasin protein-induced IL-8 and MCP-1 production in epithelial cells. INT J MED MICROBIOL, 295(8), 531-8. https://doi.org/10.1016/j.ijmm.2005.07.012

Vancouver

Grassl GA, Fessele S, Merfort I, Lindenmeyer MT, Castro V, Murillo R et al. Sesquiterpene lactones inhibit Yersinia invasin protein-induced IL-8 and MCP-1 production in epithelial cells. INT J MED MICROBIOL. 2005 Dec;295(8):531-8. https://doi.org/10.1016/j.ijmm.2005.07.012

Bibtex

@article{b305ff15069f4454bc3159ece56deb67,
title = "Sesquiterpene lactones inhibit Yersinia invasin protein-induced IL-8 and MCP-1 production in epithelial cells",
abstract = "Enteric Yersinia bacteria trigger transcription and secretion of the proinflammatory chemokines interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in epithelial cells. Both chemokines are controlled by NF-kappaB. The NF-kappaB-binding site in the IL-8 promoter preferentially binds Rel p65/p65 homodimers and p50/p65 heterodimers while the NF-kappaB-binding motifs of the MCP-1 promoter preferably bind p50/p65 heterodimers and p50/p50 homodimers. Sesquiterpene lactones inhibit the transcription factor NF-kappaB by alkylating the p65 subunit. In this study we investigated the inhibitory effects of sesquiterpene lactones and the NF-kappaB inhibitor SN50 on NF-kappaB p50 and p65 subunits in Yersinia-triggered IL-8 and MCP-1 production. The sesquiterpene lactones blocked Yersinia-triggered IL-8 and MCP-1 production in a dose-dependent manner. In contrast, SN50 inhibited IL-8 production at high concentrations whereas it diminished the amount of secreted MCP-1 significantly already at low concentrations. By means of electrophoretic mobility shift assays we demonstrate that sesquiterpene lactones inhibit Yersinia-triggered activation of NF-kappaB by inhibiting Rel p65, but not Rel p50. Our results also demonstrate that SN50 is useful for inhibition of nuclear translocation of the NF-kappaB p50 subunit but cannot be considered a general NF-kappaB inhibitor.",
keywords = "Adhesins, Bacterial, Chemokine CCL2, DNA, Electrophoretic Mobility Shift Assay, Enzyme-Linked Immunosorbent Assay, Epithelial Cells, HeLa Cells, Humans, Interleukin-8, NF-kappa B, NF-kappa B p50 Subunit, Peptides, Protein Binding, RNA, Messenger, Sesquiterpenes, Transcription Factor RelA, Yersinia enterocolitica, Journal Article",
author = "Grassl, {Guntram A} and Sabine Fessele and Irmgard Merfort and Lindenmeyer, {Maja T} and Victor Castro and Renato Murillo and Nelson, {Peter J} and Autenrieth, {Ingo B}",
year = "2005",
month = dec,
doi = "10.1016/j.ijmm.2005.07.012",
language = "English",
volume = "295",
pages = "531--8",
journal = "INT J MED MICROBIOL",
issn = "1438-4221",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "8",

}

RIS

TY - JOUR

T1 - Sesquiterpene lactones inhibit Yersinia invasin protein-induced IL-8 and MCP-1 production in epithelial cells

AU - Grassl, Guntram A

AU - Fessele, Sabine

AU - Merfort, Irmgard

AU - Lindenmeyer, Maja T

AU - Castro, Victor

AU - Murillo, Renato

AU - Nelson, Peter J

AU - Autenrieth, Ingo B

PY - 2005/12

Y1 - 2005/12

N2 - Enteric Yersinia bacteria trigger transcription and secretion of the proinflammatory chemokines interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in epithelial cells. Both chemokines are controlled by NF-kappaB. The NF-kappaB-binding site in the IL-8 promoter preferentially binds Rel p65/p65 homodimers and p50/p65 heterodimers while the NF-kappaB-binding motifs of the MCP-1 promoter preferably bind p50/p65 heterodimers and p50/p50 homodimers. Sesquiterpene lactones inhibit the transcription factor NF-kappaB by alkylating the p65 subunit. In this study we investigated the inhibitory effects of sesquiterpene lactones and the NF-kappaB inhibitor SN50 on NF-kappaB p50 and p65 subunits in Yersinia-triggered IL-8 and MCP-1 production. The sesquiterpene lactones blocked Yersinia-triggered IL-8 and MCP-1 production in a dose-dependent manner. In contrast, SN50 inhibited IL-8 production at high concentrations whereas it diminished the amount of secreted MCP-1 significantly already at low concentrations. By means of electrophoretic mobility shift assays we demonstrate that sesquiterpene lactones inhibit Yersinia-triggered activation of NF-kappaB by inhibiting Rel p65, but not Rel p50. Our results also demonstrate that SN50 is useful for inhibition of nuclear translocation of the NF-kappaB p50 subunit but cannot be considered a general NF-kappaB inhibitor.

AB - Enteric Yersinia bacteria trigger transcription and secretion of the proinflammatory chemokines interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in epithelial cells. Both chemokines are controlled by NF-kappaB. The NF-kappaB-binding site in the IL-8 promoter preferentially binds Rel p65/p65 homodimers and p50/p65 heterodimers while the NF-kappaB-binding motifs of the MCP-1 promoter preferably bind p50/p65 heterodimers and p50/p50 homodimers. Sesquiterpene lactones inhibit the transcription factor NF-kappaB by alkylating the p65 subunit. In this study we investigated the inhibitory effects of sesquiterpene lactones and the NF-kappaB inhibitor SN50 on NF-kappaB p50 and p65 subunits in Yersinia-triggered IL-8 and MCP-1 production. The sesquiterpene lactones blocked Yersinia-triggered IL-8 and MCP-1 production in a dose-dependent manner. In contrast, SN50 inhibited IL-8 production at high concentrations whereas it diminished the amount of secreted MCP-1 significantly already at low concentrations. By means of electrophoretic mobility shift assays we demonstrate that sesquiterpene lactones inhibit Yersinia-triggered activation of NF-kappaB by inhibiting Rel p65, but not Rel p50. Our results also demonstrate that SN50 is useful for inhibition of nuclear translocation of the NF-kappaB p50 subunit but cannot be considered a general NF-kappaB inhibitor.

KW - Adhesins, Bacterial

KW - Chemokine CCL2

KW - DNA

KW - Electrophoretic Mobility Shift Assay

KW - Enzyme-Linked Immunosorbent Assay

KW - Epithelial Cells

KW - HeLa Cells

KW - Humans

KW - Interleukin-8

KW - NF-kappa B

KW - NF-kappa B p50 Subunit

KW - Peptides

KW - Protein Binding

KW - RNA, Messenger

KW - Sesquiterpenes

KW - Transcription Factor RelA

KW - Yersinia enterocolitica

KW - Journal Article

U2 - 10.1016/j.ijmm.2005.07.012

DO - 10.1016/j.ijmm.2005.07.012

M3 - SCORING: Journal article

C2 - 16325549

VL - 295

SP - 531

EP - 538

JO - INT J MED MICROBIOL

JF - INT J MED MICROBIOL

SN - 1438-4221

IS - 8

ER -