Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison
Standard
Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison. / Mehrabi, P; Bücker, R; Bourenkov, G; Ginn, H M; von Stetten, D; Müller-Werkmeister, H M; Kuo, A; Morizumi, T; Eger, B T; Ou, W-L; Oghbaey, S; Sarracini, A; Besaw, J E; Pare-Labrosse, O; Meier, S; Schikora, H; Tellkamp, F; Marx, A; Sherrell, D A; Axford, D; Owen, R L; Ernst, O P; Pai, E F; Schulz, E C; Miller, R J D.
In: SCI ADV, Vol. 7, No. 12, 03.2021.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison
AU - Mehrabi, P
AU - Bücker, R
AU - Bourenkov, G
AU - Ginn, H M
AU - von Stetten, D
AU - Müller-Werkmeister, H M
AU - Kuo, A
AU - Morizumi, T
AU - Eger, B T
AU - Ou, W-L
AU - Oghbaey, S
AU - Sarracini, A
AU - Besaw, J E
AU - Pare-Labrosse, O
AU - Meier, S
AU - Schikora, H
AU - Tellkamp, F
AU - Marx, A
AU - Sherrell, D A
AU - Axford, D
AU - Owen, R L
AU - Ernst, O P
AU - Pai, E F
AU - Schulz, E C
AU - Miller, R J D
N1 - Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
PY - 2021/3
Y1 - 2021/3
N2 - For the two proteins myoglobin and fluoroacetate dehalogenase, we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of micron-sized crystals, the same sample delivery device, and the same data analysis software. Overall figures of merit indicate that the data of both radiation sources are of equivalent quality. For both proteins, reasonable data statistics can be obtained with approximately 5000 room-temperature diffraction images irrespective of the radiation source. The direct comparability of SSX and SFX data indicates that the quality of diffraction data obtained from these samples is linked to the properties of the crystals rather than to the radiation source. Therefore, for other systems with similar properties, time-resolved experiments can be conducted at the radiation source that best matches the desired time resolution.
AB - For the two proteins myoglobin and fluoroacetate dehalogenase, we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of micron-sized crystals, the same sample delivery device, and the same data analysis software. Overall figures of merit indicate that the data of both radiation sources are of equivalent quality. For both proteins, reasonable data statistics can be obtained with approximately 5000 room-temperature diffraction images irrespective of the radiation source. The direct comparability of SSX and SFX data indicates that the quality of diffraction data obtained from these samples is linked to the properties of the crystals rather than to the radiation source. Therefore, for other systems with similar properties, time-resolved experiments can be conducted at the radiation source that best matches the desired time resolution.
KW - Crystallography, X-Ray
KW - Proteins
KW - Synchrotrons
U2 - 10.1126/sciadv.abf1380
DO - 10.1126/sciadv.abf1380
M3 - SCORING: Journal article
C2 - 33731353
VL - 7
JO - SCI ADV
JF - SCI ADV
SN - 2375-2548
IS - 12
ER -