Sequence analysis and high-throughput immunohistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays.

Mona Pache; Katharina Glatz; Doris Bösch; Stephan Dirnhofer; Martina Mirlacher; Ronald Simon; Peter Schraml; Alex Rufle; Josef Flammer; Guido Sauter; Peter Meyer

Sequence analysis and high-throughput immunohistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays.

Standard

Sequence analysis and high-throughput immunohistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays. / Pache, Mona; Glatz, Katharina; Bösch, Doris; Dirnhofer, Stephan; Mirlacher, Martina; Simon, Ronald; Schraml, Peter; Rufle, Alex; Flammer, Josef; Sauter, Guido; Meyer, Peter.

In: VIRCHOWS ARCH, Vol. 443, No. 6, 6, 2003, p. 741-744.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Pache, M, Glatz, K, Bösch, D, Dirnhofer, S, Mirlacher, M, Simon, R, Schraml, P, Rufle, A, Flammer, J, Sauter, G & Meyer, P 2003, 'Sequence analysis and high-throughput immunohistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays.', VIRCHOWS ARCH, vol. 443, no. 6, 6, pp. 741-744. <http://www.ncbi.nlm.nih.gov/pubmed/14513377?dopt=Citation>

APA

Pache, M., Glatz, K., Bösch, D., Dirnhofer, S., Mirlacher, M., Simon, R., Schraml, P., Rufle, A., Flammer, J., Sauter, G., & Meyer, P. (2003). Sequence analysis and high-throughput immunohistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays. VIRCHOWS ARCH, 443(6), 741-744. [6]. http://www.ncbi.nlm.nih.gov/pubmed/14513377?dopt=Citation

Vancouver

Pache M, Glatz K, Bösch D, Dirnhofer S, Mirlacher M, Simon R et al. Sequence analysis and high-throughput immunohistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays. VIRCHOWS ARCH. 2003;443(6):741-744. 6.

Bibtex

@article{f893ac4cb73445e9900fadfb9e7754a8,

title = "Sequence analysis and high-throughput immunohistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays.",

abstract = "We aimed to immunohistochemically examine the expression of KIT (CD 117) in human posterior uveal melanoma and to analyze KIT-positive tumors for gene mutations. Brought into a tissue microarray (TMA) format were 101 formalin-fixed, paraffin-embedded posterior uveal melanomas. Immunohistochemistry was performed using the polyclonal anti-CD117 antibody from Dako (A4502). In ten selected KIT-positive tumors, exons 2, 8, 9, 11, 13 and 17 were sequenced. Of the 101 cases, 89 (88%) could be evaluated on the TMAs. Immunohistochemistry for CD 117 was weakly positive in 5 cases (6%), moderately positive in 10 cases (12%) and strongly positive in 57 cases (69%). No KIT mutations were detected in the analyzed exons. In conclusion, human posterior uveal melanoma frequently expresses CD117 at high levels. Although KIT mutations could not be found, it appears justified to investigate the utility of imatinib mesylate in the treatment of these patients.",

author = "Mona Pache and Katharina Glatz and Doris B{\"o}sch and Stephan Dirnhofer and Martina Mirlacher and Ronald Simon and Peter Schraml and Alex Rufle and Josef Flammer and Guido Sauter and Peter Meyer",

year = "2003",

language = "Deutsch",

volume = "443",

pages = "741--744",

journal = "VIRCHOWS ARCH",

issn = "0945-6317",

publisher = "Springer",

number = "6",

}

RIS

TY - JOUR

T1 - Sequence analysis and high-throughput immunohistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays.

AU - Pache, Mona

AU - Glatz, Katharina

AU - Bösch, Doris

AU - Dirnhofer, Stephan

AU - Mirlacher, Martina

AU - Simon, Ronald

AU - Schraml, Peter

AU - Rufle, Alex

AU - Flammer, Josef

AU - Sauter, Guido

AU - Meyer, Peter

PY - 2003

Y1 - 2003

N2 - We aimed to immunohistochemically examine the expression of KIT (CD 117) in human posterior uveal melanoma and to analyze KIT-positive tumors for gene mutations. Brought into a tissue microarray (TMA) format were 101 formalin-fixed, paraffin-embedded posterior uveal melanomas. Immunohistochemistry was performed using the polyclonal anti-CD117 antibody from Dako (A4502). In ten selected KIT-positive tumors, exons 2, 8, 9, 11, 13 and 17 were sequenced. Of the 101 cases, 89 (88%) could be evaluated on the TMAs. Immunohistochemistry for CD 117 was weakly positive in 5 cases (6%), moderately positive in 10 cases (12%) and strongly positive in 57 cases (69%). No KIT mutations were detected in the analyzed exons. In conclusion, human posterior uveal melanoma frequently expresses CD117 at high levels. Although KIT mutations could not be found, it appears justified to investigate the utility of imatinib mesylate in the treatment of these patients.

AB - We aimed to immunohistochemically examine the expression of KIT (CD 117) in human posterior uveal melanoma and to analyze KIT-positive tumors for gene mutations. Brought into a tissue microarray (TMA) format were 101 formalin-fixed, paraffin-embedded posterior uveal melanomas. Immunohistochemistry was performed using the polyclonal anti-CD117 antibody from Dako (A4502). In ten selected KIT-positive tumors, exons 2, 8, 9, 11, 13 and 17 were sequenced. Of the 101 cases, 89 (88%) could be evaluated on the TMAs. Immunohistochemistry for CD 117 was weakly positive in 5 cases (6%), moderately positive in 10 cases (12%) and strongly positive in 57 cases (69%). No KIT mutations were detected in the analyzed exons. In conclusion, human posterior uveal melanoma frequently expresses CD117 at high levels. Although KIT mutations could not be found, it appears justified to investigate the utility of imatinib mesylate in the treatment of these patients.

M3 - SCORING: Zeitschriftenaufsatz

VL - 443

SP - 741

EP - 744

JO - VIRCHOWS ARCH

JF - VIRCHOWS ARCH

SN - 0945-6317

IS - 6

M1 - 6

ER -