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Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol

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Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol. / Chabloz, Antoine; Schaefer, Jonas V; Kozieradzki, Ivona; Cronin, Shane J F; Strebinger, Daniel; Macaluso, Francesca; Wald, Jiri; Rabbitts, Terence H; Plückthun, Andreas; Marlovits, Thomas C; Penninger, Josef M.

In: COMMUN BIOL, Vol. 3, No. 1, 03.07.2020, p. 342.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Chabloz, A, Schaefer, JV, Kozieradzki, I, Cronin, SJF, Strebinger, D, Macaluso, F, Wald, J, Rabbitts, TH, Plückthun, A, Marlovits, TC & Penninger, JM 2020, 'Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol', COMMUN BIOL, vol. 3, no. 1, pp. 342. https://doi.org/10.1038/s42003-020-1072-4

APA

Chabloz, A., Schaefer, J. V., Kozieradzki, I., Cronin, S. J. F., Strebinger, D., Macaluso, F., Wald, J., Rabbitts, T. H., Plückthun, A., Marlovits, T. C., & Penninger, J. M. (2020). Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol. COMMUN BIOL, 3(1), 342. https://doi.org/10.1038/s42003-020-1072-4

Vancouver

Chabloz A, Schaefer JV, Kozieradzki I, Cronin SJF, Strebinger D, Macaluso F et al. Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol. COMMUN BIOL. 2020 Jul 3;3(1):342. https://doi.org/10.1038/s42003-020-1072-4

Bibtex

@article{4648335539f8455d9e05588619eb2c17,
title = "Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol",
abstract = "Protein-based affinity reagents (like antibodies or alternative binding scaffolds) offer wide-ranging applications for basic research and therapeutic approaches. However, whereas small chemical molecules efficiently reach intracellular targets, the delivery of macromolecules into the cytosol of cells remains a major challenge; thus cytosolic applications of protein-based reagents are rather limited. Some pathogenic bacteria have evolved a conserved type III secretion system (T3SS) which allows the delivery of effector proteins into eukaryotic cells. Here, we enhance the T3SS of an avirulent strain of Salmonella typhimurium to reproducibly deliver multiple classes of recombinant proteins into eukaryotic cells. The efficacy of the system is probed with both DARPins and monobodies to functionally inhibit the paradigmatic and largely undruggable RAS signaling pathway. Thus, we develop a bacterial secretion system for potent cytosolic delivery of therapeutic macromolecules.",
author = "Antoine Chabloz and Schaefer, {Jonas V} and Ivona Kozieradzki and Cronin, {Shane J F} and Daniel Strebinger and Francesca Macaluso and Jiri Wald and Rabbitts, {Terence H} and Andreas Pl{\"u}ckthun and Marlovits, {Thomas C} and Penninger, {Josef M}",
year = "2020",
month = jul,
day = "3",
doi = "10.1038/s42003-020-1072-4",
language = "English",
volume = "3",
pages = "342",
journal = "COMMUN BIOL",
issn = "2399-3642",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol

AU - Chabloz, Antoine

AU - Schaefer, Jonas V

AU - Kozieradzki, Ivona

AU - Cronin, Shane J F

AU - Strebinger, Daniel

AU - Macaluso, Francesca

AU - Wald, Jiri

AU - Rabbitts, Terence H

AU - Plückthun, Andreas

AU - Marlovits, Thomas C

AU - Penninger, Josef M

PY - 2020/7/3

Y1 - 2020/7/3

N2 - Protein-based affinity reagents (like antibodies or alternative binding scaffolds) offer wide-ranging applications for basic research and therapeutic approaches. However, whereas small chemical molecules efficiently reach intracellular targets, the delivery of macromolecules into the cytosol of cells remains a major challenge; thus cytosolic applications of protein-based reagents are rather limited. Some pathogenic bacteria have evolved a conserved type III secretion system (T3SS) which allows the delivery of effector proteins into eukaryotic cells. Here, we enhance the T3SS of an avirulent strain of Salmonella typhimurium to reproducibly deliver multiple classes of recombinant proteins into eukaryotic cells. The efficacy of the system is probed with both DARPins and monobodies to functionally inhibit the paradigmatic and largely undruggable RAS signaling pathway. Thus, we develop a bacterial secretion system for potent cytosolic delivery of therapeutic macromolecules.

AB - Protein-based affinity reagents (like antibodies or alternative binding scaffolds) offer wide-ranging applications for basic research and therapeutic approaches. However, whereas small chemical molecules efficiently reach intracellular targets, the delivery of macromolecules into the cytosol of cells remains a major challenge; thus cytosolic applications of protein-based reagents are rather limited. Some pathogenic bacteria have evolved a conserved type III secretion system (T3SS) which allows the delivery of effector proteins into eukaryotic cells. Here, we enhance the T3SS of an avirulent strain of Salmonella typhimurium to reproducibly deliver multiple classes of recombinant proteins into eukaryotic cells. The efficacy of the system is probed with both DARPins and monobodies to functionally inhibit the paradigmatic and largely undruggable RAS signaling pathway. Thus, we develop a bacterial secretion system for potent cytosolic delivery of therapeutic macromolecules.

U2 - 10.1038/s42003-020-1072-4

DO - 10.1038/s42003-020-1072-4

M3 - SCORING: Journal article

C2 - 32620833

VL - 3

SP - 342

JO - COMMUN BIOL

JF - COMMUN BIOL

SN - 2399-3642

IS - 1

ER -