Roles of 17ß-estradiol involve regulation of reelin expression and synaptogenesis in the dentate gyrus.
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Roles of 17ß-estradiol involve regulation of reelin expression and synaptogenesis in the dentate gyrus. / Bender, Roland; Zhou, Lepu; Wilkars, Wiebke; Fester, Lars; Lanowski, Jan-Simon; Paysen, Danny; König, Almut; Rune, Gabriele M.
In: CEREB CORTEX, Vol. 20, No. 12, 12, 2010, p. 2985-2995.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Roles of 17ß-estradiol involve regulation of reelin expression and synaptogenesis in the dentate gyrus.
AU - Bender, Roland
AU - Zhou, Lepu
AU - Wilkars, Wiebke
AU - Fester, Lars
AU - Lanowski, Jan-Simon
AU - Paysen, Danny
AU - König, Almut
AU - Rune, Gabriele M.
PY - 2010
Y1 - 2010
N2 - Studies on the role of 17 -estradiol (E2) in the hippocampus have mainly focused on CA1 and CA3 regions, whereas in dentate gyrus (DG), its role is largely unknown. Here, we examined potential functions of E2 in DG, particularly during development. Immunohistochemistry and in situ hybridization revealed abundance of estrogen receptor (ER) , but not ER , expression in DG. Similar to CA1, analysis of synapse densities revealed a reduction in spine synapse number in DG molecular layer of immature rats and adult mice after inhibition of estradiol synthesis using letrozole. Interestingly, strong expression of ER was found in Cajal-Retzius (CR) cells, which regulate neuronal migration and synaptogenesis via the extracellular matrix protein reelin. Immunoreactivity of aromatase, the final enzyme of estradiol synthesis, was strongest in mature granule cells. In hippocampal slice cultures, exogenous application of E2 caused an increase in reelin expression in CR cells, which was abolished after blockade of ERs using ICI182,780. Vice versa, inhibition of aromatase activity by letrozole resulted in reduced reelin expression, suggesting that E2 deriving from hippocampal sources contributes to the regulation of reelin as well as to the maintenance of spine synapses in DG. E2 further regulated Notch1, a signaling protein involved in neuronal differentiation.
AB - Studies on the role of 17 -estradiol (E2) in the hippocampus have mainly focused on CA1 and CA3 regions, whereas in dentate gyrus (DG), its role is largely unknown. Here, we examined potential functions of E2 in DG, particularly during development. Immunohistochemistry and in situ hybridization revealed abundance of estrogen receptor (ER) , but not ER , expression in DG. Similar to CA1, analysis of synapse densities revealed a reduction in spine synapse number in DG molecular layer of immature rats and adult mice after inhibition of estradiol synthesis using letrozole. Interestingly, strong expression of ER was found in Cajal-Retzius (CR) cells, which regulate neuronal migration and synaptogenesis via the extracellular matrix protein reelin. Immunoreactivity of aromatase, the final enzyme of estradiol synthesis, was strongest in mature granule cells. In hippocampal slice cultures, exogenous application of E2 caused an increase in reelin expression in CR cells, which was abolished after blockade of ERs using ICI182,780. Vice versa, inhibition of aromatase activity by letrozole resulted in reduced reelin expression, suggesting that E2 deriving from hippocampal sources contributes to the regulation of reelin as well as to the maintenance of spine synapses in DG. E2 further regulated Notch1, a signaling protein involved in neuronal differentiation.
M3 - SCORING: Zeitschriftenaufsatz
VL - 20
SP - 2985
EP - 2995
JO - CEREB CORTEX
JF - CEREB CORTEX
SN - 1047-3211
IS - 12
M1 - 12
ER -