Role of p-selectin in platelet sequestration in pulmonary capillaries during endotoxemia.
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Role of p-selectin in platelet sequestration in pulmonary capillaries during endotoxemia. / Kiefmann, Rainer; Heckel, Kai; Schenkat, Sonja; Dörger, Martina; Goetz, Alwin E.
In: J VASC RES, Vol. 43, No. 5, 5, 2006, p. 473-481.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Role of p-selectin in platelet sequestration in pulmonary capillaries during endotoxemia.
AU - Kiefmann, Rainer
AU - Heckel, Kai
AU - Schenkat, Sonja
AU - Dörger, Martina
AU - Goetz, Alwin E.
PY - 2006
Y1 - 2006
N2 - BACKGROUND: There is growing evidence that platelets accumulate in the lung and contribute to the pathogenesis of acute lung injury during endotoxemia. The aims of the present study were to localize platelet sequestration in the pulmonary microcirculation and to investigate the role of P-selectin as a molecular mechanism of platelet endothelial cell interaction. METHODS: We used in vivo fluorescence microscopy to quantify the kinetics of fluorescently labeled erythrocytes and platelets in alveolar capillary networks in rabbit lungs. RESULTS: Six hours after onset of endotoxin infusion we observed a massive rolling along and firm adherence of platelets to lung capillary endothelial cells whereas under control conditions no platelet sequestration was detected. P-selectin was expressed on the surface of separated platelets which were incubated with endotoxin and in lung tissue. Pretreatment of platelets with fucoidin, a P-selectin antagonist, significantly attenuated the endotoxin-induced platelet rolling and adherence. In contrast, intravenous infusion of fucoidin in endotoxin-treated rabbits did not inhibit platelet sequestration in pulmonary capillaries. CONCLUSION: We conclude that platelets accumulate in alveolar capillaries following endotoxemia. P-selectin expressed on the surface of platelets seems to play an important role in mediating this platelet-endothelial cell interaction.
AB - BACKGROUND: There is growing evidence that platelets accumulate in the lung and contribute to the pathogenesis of acute lung injury during endotoxemia. The aims of the present study were to localize platelet sequestration in the pulmonary microcirculation and to investigate the role of P-selectin as a molecular mechanism of platelet endothelial cell interaction. METHODS: We used in vivo fluorescence microscopy to quantify the kinetics of fluorescently labeled erythrocytes and platelets in alveolar capillary networks in rabbit lungs. RESULTS: Six hours after onset of endotoxin infusion we observed a massive rolling along and firm adherence of platelets to lung capillary endothelial cells whereas under control conditions no platelet sequestration was detected. P-selectin was expressed on the surface of separated platelets which were incubated with endotoxin and in lung tissue. Pretreatment of platelets with fucoidin, a P-selectin antagonist, significantly attenuated the endotoxin-induced platelet rolling and adherence. In contrast, intravenous infusion of fucoidin in endotoxin-treated rabbits did not inhibit platelet sequestration in pulmonary capillaries. CONCLUSION: We conclude that platelets accumulate in alveolar capillaries following endotoxemia. P-selectin expressed on the surface of platelets seems to play an important role in mediating this platelet-endothelial cell interaction.
M3 - SCORING: Zeitschriftenaufsatz
VL - 43
SP - 473
EP - 481
IS - 5
M1 - 5
ER -
