Research ExplorerPublicationsRole of KCNMA1 in breast cancer.

Role of KCNMA1 in breast cancer.

Standard

Role of KCNMA1 in breast cancer. / Oeggerli, Martin; Tian, Yuemin; Ruiz, Christian; Wijker, Barbara; Sauter, Guido; Obermann, Ellen; Güth, Uwe; Zlobec, Inti; Sausbier, Matthias; Kunzelmann, Karl; Bubendorf, Lukas.

In: PLOS ONE, Vol. 7, No. 8, 8, 2012, p. 41664.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Oeggerli, M, Tian, Y, Ruiz, C, Wijker, B, Sauter, G, Obermann, E, Güth, U, Zlobec, I, Sausbier, M, Kunzelmann, K & Bubendorf, L 2012, 'Role of KCNMA1 in breast cancer.', PLOS ONE, vol. 7, no. 8, 8, pp. 41664. https://doi.org/10.1371/journal.pone.0041664

APA

Oeggerli, M., Tian, Y., Ruiz, C., Wijker, B., Sauter, G., Obermann, E., Güth, U., Zlobec, I., Sausbier, M., Kunzelmann, K., & Bubendorf, L. (2012). Role of KCNMA1 in breast cancer. PLOS ONE, 7(8), 41664. [8]. https://doi.org/10.1371/journal.pone.0041664

Vancouver

Oeggerli M, Tian Y, Ruiz C, Wijker B, Sauter G, Obermann E et al. Role of KCNMA1 in breast cancer. PLOS ONE. 2012;7(8):41664. 8. https://doi.org/10.1371/journal.pone.0041664

Bibtex

@article{5c3ad5a9282d4e648b3e94976a23779f,
title = "Role of KCNMA1 in breast cancer.",
abstract = "KCNMA1 encodes the ?-subunit of the large conductance, voltage and Ca(2+)-activated (BK) potassium channel and has been reported as a target gene of genomic amplification at 10q22 in prostate cancer. To investigate the prevalence of the amplification in other human cancers, the copy number of KCNMA1 was analyzed by fluorescence-in-situ-hybridization (FISH) in 2,445 tumors across 118 different tumor types. Amplification of KCNMA1 was restricted to a small but distinct fraction of breast, ovarian and endometrial cancer with the highest prevalence in invasive ductal breast cancers and serous carcinoma of ovary and endometrium (3-7%). We performed an extensive analysis on breast cancer tissue microarrays (TMA) of 1,200 tumors linked to prognosis. KCNMA1 amplification was significantly associated with high tumor stage, high grade, high tumor cell proliferation, and poor prognosis. Immunofluorescence revealed moderate or strong KCNMA1 protein expression in 8 out of 9 human breast cancers and in the breast cancer cell line MFM223. KCNMA1-function in breast cancer cell lines was confirmed by whole-cell patch clamp recordings and proliferation assays, using siRNA-knockdown, BK channel activators such as 17{\ss}-estradiol and the BK-channel blocker paxilline. Our findings revealed that enhanced expression of KCNMA1 correlates with and contributes to high proliferation rate and malignancy of breast cancer.",
keywords = "Adult, Humans, Male, Aged, Female, Middle Aged, Aged, 80 and over, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Cell Proliferation, Gene Amplification, In Situ Hybridization, Fluorescence, RNA Interference, Breast Neoplasms/*genetics/metabolism/pathology, Estrogen Receptor alpha/genetics/metabolism, Receptors, Progesterone/genetics/metabolism, Ki-67 Antigen/genetics/metabolism, Large-Conductance Calcium-Activated Potassium Channel alpha Subunits/*genetics/metabolism, MCF-7 Cells, Prostatic Neoplasms/genetics/metabolism, Adult, Humans, Male, Aged, Female, Middle Aged, Aged, 80 and over, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Cell Proliferation, Gene Amplification, In Situ Hybridization, Fluorescence, RNA Interference, Breast Neoplasms/*genetics/metabolism/pathology, Estrogen Receptor alpha/genetics/metabolism, Receptors, Progesterone/genetics/metabolism, Ki-67 Antigen/genetics/metabolism, Large-Conductance Calcium-Activated Potassium Channel alpha Subunits/*genetics/metabolism, MCF-7 Cells, Prostatic Neoplasms/genetics/metabolism",
author = "Martin Oeggerli and Yuemin Tian and Christian Ruiz and Barbara Wijker and Guido Sauter and Ellen Obermann and Uwe G{\"u}th and Inti Zlobec and Matthias Sausbier and Karl Kunzelmann and Lukas Bubendorf",
year = "2012",
doi = "10.1371/journal.pone.0041664",
language = "English",
volume = "7",
pages = "41664",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

RIS

TY - JOUR

T1 - Role of KCNMA1 in breast cancer.

AU - Oeggerli, Martin

AU - Tian, Yuemin

AU - Ruiz, Christian

AU - Wijker, Barbara

AU - Sauter, Guido

AU - Obermann, Ellen

AU - Güth, Uwe

AU - Zlobec, Inti

AU - Sausbier, Matthias

AU - Kunzelmann, Karl

AU - Bubendorf, Lukas

PY - 2012

Y1 - 2012

N2 - KCNMA1 encodes the ?-subunit of the large conductance, voltage and Ca(2+)-activated (BK) potassium channel and has been reported as a target gene of genomic amplification at 10q22 in prostate cancer. To investigate the prevalence of the amplification in other human cancers, the copy number of KCNMA1 was analyzed by fluorescence-in-situ-hybridization (FISH) in 2,445 tumors across 118 different tumor types. Amplification of KCNMA1 was restricted to a small but distinct fraction of breast, ovarian and endometrial cancer with the highest prevalence in invasive ductal breast cancers and serous carcinoma of ovary and endometrium (3-7%). We performed an extensive analysis on breast cancer tissue microarrays (TMA) of 1,200 tumors linked to prognosis. KCNMA1 amplification was significantly associated with high tumor stage, high grade, high tumor cell proliferation, and poor prognosis. Immunofluorescence revealed moderate or strong KCNMA1 protein expression in 8 out of 9 human breast cancers and in the breast cancer cell line MFM223. KCNMA1-function in breast cancer cell lines was confirmed by whole-cell patch clamp recordings and proliferation assays, using siRNA-knockdown, BK channel activators such as 17ß-estradiol and the BK-channel blocker paxilline. Our findings revealed that enhanced expression of KCNMA1 correlates with and contributes to high proliferation rate and malignancy of breast cancer.

AB - KCNMA1 encodes the ?-subunit of the large conductance, voltage and Ca(2+)-activated (BK) potassium channel and has been reported as a target gene of genomic amplification at 10q22 in prostate cancer. To investigate the prevalence of the amplification in other human cancers, the copy number of KCNMA1 was analyzed by fluorescence-in-situ-hybridization (FISH) in 2,445 tumors across 118 different tumor types. Amplification of KCNMA1 was restricted to a small but distinct fraction of breast, ovarian and endometrial cancer with the highest prevalence in invasive ductal breast cancers and serous carcinoma of ovary and endometrium (3-7%). We performed an extensive analysis on breast cancer tissue microarrays (TMA) of 1,200 tumors linked to prognosis. KCNMA1 amplification was significantly associated with high tumor stage, high grade, high tumor cell proliferation, and poor prognosis. Immunofluorescence revealed moderate or strong KCNMA1 protein expression in 8 out of 9 human breast cancers and in the breast cancer cell line MFM223. KCNMA1-function in breast cancer cell lines was confirmed by whole-cell patch clamp recordings and proliferation assays, using siRNA-knockdown, BK channel activators such as 17ß-estradiol and the BK-channel blocker paxilline. Our findings revealed that enhanced expression of KCNMA1 correlates with and contributes to high proliferation rate and malignancy of breast cancer.

KW - Adult

KW - Humans

KW - Male

KW - Aged

KW - Female

KW - Middle Aged

KW - Aged, 80 and over

KW - Cell Line, Tumor

KW - Gene Expression Regulation, Neoplastic

KW - Cell Proliferation

KW - Gene Amplification

KW - In Situ Hybridization, Fluorescence

KW - RNA Interference

KW - Breast Neoplasms/genetics/metabolism/pathology

KW - Estrogen Receptor alpha/genetics/metabolism

KW - Receptors, Progesterone/genetics/metabolism

KW - Ki-67 Antigen/genetics/metabolism

KW - Large-Conductance Calcium-Activated Potassium Channel alpha Subunits/genetics/metabolism

KW - MCF-7 Cells

KW - Prostatic Neoplasms/genetics/metabolism

KW - Adult

KW - Humans

KW - Male

KW - Aged

KW - Female

KW - Middle Aged

KW - Aged, 80 and over

KW - Cell Line, Tumor

KW - Gene Expression Regulation, Neoplastic

KW - Cell Proliferation

KW - Gene Amplification

KW - In Situ Hybridization, Fluorescence

KW - RNA Interference

KW - Breast Neoplasms/genetics/metabolism/pathology

KW - Estrogen Receptor alpha/genetics/metabolism

KW - Receptors, Progesterone/genetics/metabolism

KW - Ki-67 Antigen/genetics/metabolism

KW - Large-Conductance Calcium-Activated Potassium Channel alpha Subunits/genetics/metabolism

KW - MCF-7 Cells

KW - Prostatic Neoplasms/genetics/metabolism

U2 - 10.1371/journal.pone.0041664

DO - 10.1371/journal.pone.0041664

M3 - SCORING: Journal article

VL - 7

SP - 41664

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 8

M1 - 8

ER -