Role for Reelin-induced cofilin phosphorylation in the assembly of sympathetic preganglionic neurons in the murine intermediolateral column.
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Role for Reelin-induced cofilin phosphorylation in the assembly of sympathetic preganglionic neurons in the murine intermediolateral column. / Krüger, Marie T; Zhao, Shanting; Chai, Xuejun; Brunne, Bianka; Bouché, Elisabeth; Bock, Hans H; Frotscher, Michael.
In: EUR J NEUROSCI, Vol. 32, No. 10, 10, 2010, p. 1611-1617.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Role for Reelin-induced cofilin phosphorylation in the assembly of sympathetic preganglionic neurons in the murine intermediolateral column.
AU - Krüger, Marie T
AU - Zhao, Shanting
AU - Chai, Xuejun
AU - Brunne, Bianka
AU - Bouché, Elisabeth
AU - Bock, Hans H
AU - Frotscher, Michael
PY - 2010
Y1 - 2010
N2 - Sympathetic preganglionic neurons (SPNs) are located in the intermediolateral column (IMLC) of the spinal cord. This specific localization results from primary and secondary migratory processes during spinal cord development. Thus, following neurogenesis in the neuroepithelium, SPNs migrate first in a ventrolateral direction and then, in a secondary step, dorsolaterally to reach the IMLC. These migratory processes are controlled, at least in part, by the glycoprotein Reelin, which is known to be important for the development of laminated brain structures. In reeler mutants deficient in Reelin, SPNs initially migrate ventrolaterally as normal. However, most of them then migrate medially to become eventually located near the central canal. Here, we provide evidence that in wild-type animals this aberrant medial migration towards the central canal is prevented by Reelin-induced cytoskeletal stabilization, brought about by phosphorylation of cofilin. Cofilin plays an important role in actin depolymerization, a process required for the changes in cell shape during migration. Phosphorylation of cofilin renders it unable to depolymerize F-actin, thereby stabilizing the cytoskeleton. Using immunostaining for phosphorylated cofilin (p-cofilin), we demonstrate that SPNs in wild-type animals, but not in reeler mutants and other mutants of the Reelin signalling cascade, are immunoreactive for p-cofilin. These findings suggest that Reelin near the central canal induces cofilin phosphorylation in SPNs, thereby preventing them from aberrant migration towards the central canal. The results extend our previous studies on cortical neurons in which Reelin in the marginal zone was found to stabilize the leading processes of migrating neurons and terminate the migration process.
AB - Sympathetic preganglionic neurons (SPNs) are located in the intermediolateral column (IMLC) of the spinal cord. This specific localization results from primary and secondary migratory processes during spinal cord development. Thus, following neurogenesis in the neuroepithelium, SPNs migrate first in a ventrolateral direction and then, in a secondary step, dorsolaterally to reach the IMLC. These migratory processes are controlled, at least in part, by the glycoprotein Reelin, which is known to be important for the development of laminated brain structures. In reeler mutants deficient in Reelin, SPNs initially migrate ventrolaterally as normal. However, most of them then migrate medially to become eventually located near the central canal. Here, we provide evidence that in wild-type animals this aberrant medial migration towards the central canal is prevented by Reelin-induced cytoskeletal stabilization, brought about by phosphorylation of cofilin. Cofilin plays an important role in actin depolymerization, a process required for the changes in cell shape during migration. Phosphorylation of cofilin renders it unable to depolymerize F-actin, thereby stabilizing the cytoskeleton. Using immunostaining for phosphorylated cofilin (p-cofilin), we demonstrate that SPNs in wild-type animals, but not in reeler mutants and other mutants of the Reelin signalling cascade, are immunoreactive for p-cofilin. These findings suggest that Reelin near the central canal induces cofilin phosphorylation in SPNs, thereby preventing them from aberrant migration towards the central canal. The results extend our previous studies on cortical neurons in which Reelin in the marginal zone was found to stabilize the leading processes of migrating neurons and terminate the migration process.
KW - Animals
KW - Mice
KW - Mice, Knockout
KW - Staining and Labeling methods
KW - histology
KW - Phosphorylation
KW - Neurons cytology
KW - Mice, Neurologic Mutants
KW - Receptors, LDL genetics
KW - Cell Adhesion Molecules, Neuronal genetics
KW - Extracellular Matrix Proteins genetics
KW - Nerve Tissue Proteins genetics
KW - Serine Endopeptidases genetics
KW - Actin Depolymerizing Factors genetics
KW - Autonomic Fibers, Preganglionic metabolism
KW - Ganglia, Sympathetic cytology
KW - Spinal Cord anatomy
KW - Animals
KW - Mice
KW - Mice, Knockout
KW - Staining and Labeling methods
KW - histology
KW - Phosphorylation
KW - Neurons cytology
KW - Mice, Neurologic Mutants
KW - Receptors, LDL genetics
KW - Cell Adhesion Molecules, Neuronal genetics
KW - Extracellular Matrix Proteins genetics
KW - Nerve Tissue Proteins genetics
KW - Serine Endopeptidases genetics
KW - Actin Depolymerizing Factors genetics
KW - Autonomic Fibers, Preganglionic metabolism
KW - Ganglia, Sympathetic cytology
KW - Spinal Cord anatomy
M3 - SCORING: Zeitschriftenaufsatz
VL - 32
SP - 1611
EP - 1617
JO - EUR J NEUROSCI
JF - EUR J NEUROSCI
SN - 0953-816X
IS - 10
M1 - 10
ER -
