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RNAi technology to block the expression of molecules relevant to metastasis: the cell adhesion molecule CEACAM1 as an instructive example.

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RNAi technology to block the expression of molecules relevant to metastasis: the cell adhesion molecule CEACAM1 as an instructive example. / Wicklein, Daniel.

In: Methods Mol Biol, Vol. 878, 2012, p. 241-250.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wicklein, D 2012, 'RNAi technology to block the expression of molecules relevant to metastasis: the cell adhesion molecule CEACAM1 as an instructive example.', Methods Mol Biol, vol. 878, pp. 241-250. <http://www.ncbi.nlm.nih.gov/pubmed/22674138?dopt=Citation>

APA

Wicklein, D. (2012). RNAi technology to block the expression of molecules relevant to metastasis: the cell adhesion molecule CEACAM1 as an instructive example. Methods Mol Biol, 878, 241-250. http://www.ncbi.nlm.nih.gov/pubmed/22674138?dopt=Citation

Vancouver

Wicklein D. RNAi technology to block the expression of molecules relevant to metastasis: the cell adhesion molecule CEACAM1 as an instructive example. Methods Mol Biol. 2012;878:241-250.

Bibtex

@article{36eed77bd28c40a0beac93a3a5474eac,
title = "RNAi technology to block the expression of molecules relevant to metastasis: the cell adhesion molecule CEACAM1 as an instructive example.",
abstract = "Specific gene silencing using small hairpin RNA (shRNA) constructs offer researchers the possibility to study the influence of a single protein in the metastatic process. The role of the cellular adhesion molecule CEACAM1 on tumour formation and metastasis is of some interest. The human melanoma cell line FemX-1 was transfected with an shRNA construct directed against the CEACAM family. Stable clones were obtained and characterized via puromycin selection, single-cell dilution, and subsequent FACS analysis. The cell line showed a knock-down of CEACAM1 of more than 85%. This knock-down remained stable when examined in an SCID mouse xenograft experiment over 40 days.",
keywords = "Humans, Cell Line, Tumor, Gene Knockdown Techniques, Transfection, Gene Expression Regulation, Neoplastic/*genetics, Antigens, CD/*genetics, RNA, Small Interfering/genetics, Genetic Vectors/genetics, *RNA Interference, Cell Adhesion Molecules/*deficiency/*genetics, *Genetic Techniques, Neoplasm Metastasis/*genetics, Humans, Cell Line, Tumor, Gene Knockdown Techniques, Transfection, Gene Expression Regulation, Neoplastic/*genetics, Antigens, CD/*genetics, RNA, Small Interfering/genetics, Genetic Vectors/genetics, *RNA Interference, Cell Adhesion Molecules/*deficiency/*genetics, *Genetic Techniques, Neoplasm Metastasis/*genetics",
author = "Daniel Wicklein",
year = "2012",
language = "English",
volume = "878",
pages = "241--250",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - RNAi technology to block the expression of molecules relevant to metastasis: the cell adhesion molecule CEACAM1 as an instructive example.

AU - Wicklein, Daniel

PY - 2012

Y1 - 2012

N2 - Specific gene silencing using small hairpin RNA (shRNA) constructs offer researchers the possibility to study the influence of a single protein in the metastatic process. The role of the cellular adhesion molecule CEACAM1 on tumour formation and metastasis is of some interest. The human melanoma cell line FemX-1 was transfected with an shRNA construct directed against the CEACAM family. Stable clones were obtained and characterized via puromycin selection, single-cell dilution, and subsequent FACS analysis. The cell line showed a knock-down of CEACAM1 of more than 85%. This knock-down remained stable when examined in an SCID mouse xenograft experiment over 40 days.

AB - Specific gene silencing using small hairpin RNA (shRNA) constructs offer researchers the possibility to study the influence of a single protein in the metastatic process. The role of the cellular adhesion molecule CEACAM1 on tumour formation and metastasis is of some interest. The human melanoma cell line FemX-1 was transfected with an shRNA construct directed against the CEACAM family. Stable clones were obtained and characterized via puromycin selection, single-cell dilution, and subsequent FACS analysis. The cell line showed a knock-down of CEACAM1 of more than 85%. This knock-down remained stable when examined in an SCID mouse xenograft experiment over 40 days.

KW - Humans

KW - Cell Line, Tumor

KW - Gene Knockdown Techniques

KW - Transfection

KW - Gene Expression Regulation, Neoplastic/genetics

KW - Antigens, CD/genetics

KW - RNA, Small Interfering/genetics

KW - Genetic Vectors/genetics

KW - RNA Interference

KW - Cell Adhesion Molecules/deficiency/genetics

KW - Genetic Techniques

KW - Neoplasm Metastasis/genetics

KW - Humans

KW - Cell Line, Tumor

KW - Gene Knockdown Techniques

KW - Transfection

KW - Gene Expression Regulation, Neoplastic/genetics

KW - Antigens, CD/genetics

KW - RNA, Small Interfering/genetics

KW - Genetic Vectors/genetics

KW - RNA Interference

KW - Cell Adhesion Molecules/deficiency/genetics

KW - Genetic Techniques

KW - Neoplasm Metastasis/genetics

M3 - SCORING: Journal article

VL - 878

SP - 241

EP - 250

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -