RNAemia Corresponds to Disease Severity and Antibody Response in Hospitalized COVID-19 Patients
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RNAemia Corresponds to Disease Severity and Antibody Response in Hospitalized COVID-19 Patients. / Eberhardt, Kirsten Alexandra; Meyer-Schwickerath, Charlotte; Heger, Eva; Knops, Elena; Lehmann, Clara; Rybniker, Jan; Schommers, Philipp; Eichenauer, Dennis A.; Kurth, Florian; Ramharter, Michael; Kaiser, Rolf; Holtick, Udo; Klein, Florian; Jung, Norma; Di Cristanziano, Veronica.
In: VIRUSES-BASEL, Vol. 12, No. 9, 1045, 18.09.2020.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - RNAemia Corresponds to Disease Severity and Antibody Response in Hospitalized COVID-19 Patients
AU - Eberhardt, Kirsten Alexandra
AU - Meyer-Schwickerath, Charlotte
AU - Heger, Eva
AU - Knops, Elena
AU - Lehmann, Clara
AU - Rybniker, Jan
AU - Schommers, Philipp
AU - Eichenauer, Dennis A.
AU - Kurth, Florian
AU - Ramharter, Michael
AU - Kaiser, Rolf
AU - Holtick, Udo
AU - Klein, Florian
AU - Jung, Norma
AU - Di Cristanziano, Veronica
N1 - Funding Information: We are thankful to the patients who contributed to this study. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/9/18
Y1 - 2020/9/18
N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represents a global health emergency. To improve the understanding of the systemic component of SARS-CoV-2, we investigated if viral load dynamics in plasma and respiratory samples are associated with antibody response and severity of coronavirus disease 2019 (COVID-19). SARS-CoV-2 RNA was found in plasma samples from 14 (44%) out of 32 patients. RNAemia was detected in 5 out of 6 fatal cases. Peak IgG values were significantly lower in mild/moderate than in severe (0.6 (interquartile range, IQR, 0.4–3.2) vs. 11.8 (IQR, 9.9–13.0), adjusted p = 0.003) or critical cases (11.29 (IQR, 8.3–12.0), adjusted p = 0.042). IgG titers were significantly associated with virus Ct (Cycle threshold) value in plasma and respiratory specimens ((ß = 0.4, 95% CI (confidence interval, 0.2; 0.5), p < 0.001 and ß = 0.5, 95% CI (0.2; 0.6), p = 0.002). A classification as severe or a critical case was additionally inversely associated with Ct values in plasma in comparison to mild/moderate cases (ß = −3.3, 95% CI (−5.8; 0.8), p = 0.024 and ß = −4.4, 95% CI (−7.2; 1.6), p = 0.007, respectively). Based on the present data, our hypothesis is that the early stage of SARS-CoV-2 infection is characterized by a primary RNAemia, as a potential manifestation of a systemic infection. Additionally, the viral load in plasma seems to be associated with a worse disease outcome.
AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represents a global health emergency. To improve the understanding of the systemic component of SARS-CoV-2, we investigated if viral load dynamics in plasma and respiratory samples are associated with antibody response and severity of coronavirus disease 2019 (COVID-19). SARS-CoV-2 RNA was found in plasma samples from 14 (44%) out of 32 patients. RNAemia was detected in 5 out of 6 fatal cases. Peak IgG values were significantly lower in mild/moderate than in severe (0.6 (interquartile range, IQR, 0.4–3.2) vs. 11.8 (IQR, 9.9–13.0), adjusted p = 0.003) or critical cases (11.29 (IQR, 8.3–12.0), adjusted p = 0.042). IgG titers were significantly associated with virus Ct (Cycle threshold) value in plasma and respiratory specimens ((ß = 0.4, 95% CI (confidence interval, 0.2; 0.5), p < 0.001 and ß = 0.5, 95% CI (0.2; 0.6), p = 0.002). A classification as severe or a critical case was additionally inversely associated with Ct values in plasma in comparison to mild/moderate cases (ß = −3.3, 95% CI (−5.8; 0.8), p = 0.024 and ß = −4.4, 95% CI (−7.2; 1.6), p = 0.007, respectively). Based on the present data, our hypothesis is that the early stage of SARS-CoV-2 infection is characterized by a primary RNAemia, as a potential manifestation of a systemic infection. Additionally, the viral load in plasma seems to be associated with a worse disease outcome.
KW - Antibodies
KW - Blood
KW - Dynamics
KW - Humoral response
KW - SARS-CoV-2
KW - Seroconversion
KW - Viral load
KW - Viremia
UR - http://www.scopus.com/inward/record.url?scp=85091585873&partnerID=8YFLogxK
U2 - 10.3390/v12091045
DO - 10.3390/v12091045
M3 - SCORING: Journal article
C2 - 32962125
AN - SCOPUS:85091585873
VL - 12
JO - VIRUSES-BASEL
JF - VIRUSES-BASEL
SN - 1999-4915
IS - 9
M1 - 1045
ER -