Risk factors for donor cornea contamination: retrospective analysis of 4546 procured corneas in a single eye bank.
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Risk factors for donor cornea contamination: retrospective analysis of 4546 procured corneas in a single eye bank. / Linke, Stephan J; Fricke, Otto H; Eddy, Mau-Thek; Bednarz, Jürgen; Druchkiv, Vasyl; Kaulfers, Paul-Michael; Wulff, Birgit; Püschel, Klaus; Richard, Gisbert; Hellwinkel, Olaf J C.
In: CORNEA, Vol. 32, No. 2, 2, 2013, p. 141-148.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Risk factors for donor cornea contamination: retrospective analysis of 4546 procured corneas in a single eye bank.
AU - Linke, Stephan J
AU - Fricke, Otto H
AU - Eddy, Mau-Thek
AU - Bednarz, Jürgen
AU - Druchkiv, Vasyl
AU - Kaulfers, Paul-Michael
AU - Wulff, Birgit
AU - Püschel, Klaus
AU - Richard, Gisbert
AU - Hellwinkel, Olaf J C
PY - 2013
Y1 - 2013
N2 - PURPOSE: Microbiological contamination is a common cause for elimination of organ-cultured donor corneas. The aims of the present study were to analyze contamination rates and identify risk factors for contamination.METHODS: Retrospectively, the contamination rates of 4546 organ-cultured corneas and the causative species were studied. The impact of sex, age, death-to-explantation interval, explantation technique, cause of death, and mean monthly temperature on contamination rate was analyzed.RESULTS: The median annual contamination rate was 5.3% (range: 3%-19%). Most contaminations were of fungal origin (61.9%), with Candida species (45%) being predominant. Bacterial contaminations (34.4%) were dominated by Staphylococcus species (12.8%). Sex, donor age, and mean monthly temperature had no statistically significant influence on the contamination rate. The median death-to-explantation interval of contaminated corneas (44 hours) was longer than that of sterile corneas (39 hours; P < 0.001; n = 4437). Cardiopulmonary failure was associated with the highest contamination rate (13.6%) of all death causes. The switch from whole globe to in situ excision was followed by a temporary increase in contamination rate (12.5%-19.4%).CONCLUSIONS: Although the genesis of donor cornea contamination seems to be multifactorial, resident species from physiological skin flora are the main contaminants indicating that the donor corpses could be the main source of microbiological contamination. A change in the explantation technique was followed by an increase in the contamination rate.
AB - PURPOSE: Microbiological contamination is a common cause for elimination of organ-cultured donor corneas. The aims of the present study were to analyze contamination rates and identify risk factors for contamination.METHODS: Retrospectively, the contamination rates of 4546 organ-cultured corneas and the causative species were studied. The impact of sex, age, death-to-explantation interval, explantation technique, cause of death, and mean monthly temperature on contamination rate was analyzed.RESULTS: The median annual contamination rate was 5.3% (range: 3%-19%). Most contaminations were of fungal origin (61.9%), with Candida species (45%) being predominant. Bacterial contaminations (34.4%) were dominated by Staphylococcus species (12.8%). Sex, donor age, and mean monthly temperature had no statistically significant influence on the contamination rate. The median death-to-explantation interval of contaminated corneas (44 hours) was longer than that of sterile corneas (39 hours; P < 0.001; n = 4437). Cardiopulmonary failure was associated with the highest contamination rate (13.6%) of all death causes. The switch from whole globe to in situ excision was followed by a temporary increase in contamination rate (12.5%-19.4%).CONCLUSIONS: Although the genesis of donor cornea contamination seems to be multifactorial, resident species from physiological skin flora are the main contaminants indicating that the donor corpses could be the main source of microbiological contamination. A change in the explantation technique was followed by an increase in the contamination rate.
KW - Adult
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Risk Factors
KW - Prevalence
KW - Retrospective Studies
KW - Organ Culture Techniques
KW - Cell Count
KW - Tissue and Organ Procurement
KW - Culture Media
KW - Seasons
KW - Tissue Donors/statistics & numerical data
KW - Bacteria/isolation & purification
KW - Cornea/microbiology
KW - Corneal Transplantation
KW - Endothelium, Corneal/pathology
KW - Eye Banks/statistics & numerical data
KW - Fungi/isolation & purification
KW - Organ Preservation/methods
KW - Adult
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Risk Factors
KW - Prevalence
KW - Retrospective Studies
KW - Organ Culture Techniques
KW - Cell Count
KW - Tissue and Organ Procurement
KW - Culture Media
KW - Seasons
KW - Tissue Donors/statistics & numerical data
KW - Bacteria/isolation & purification
KW - Cornea/microbiology
KW - Corneal Transplantation
KW - Endothelium, Corneal/pathology
KW - Eye Banks/statistics & numerical data
KW - Fungi/isolation & purification
KW - Organ Preservation/methods
U2 - 10.1097/ICO.0b013e31825d586b
DO - 10.1097/ICO.0b013e31825d586b
M3 - SCORING: Journal article
C2 - 22968360
VL - 32
SP - 141
EP - 148
JO - CORNEA
JF - CORNEA
SN - 0277-3740
IS - 2
M1 - 2
ER -
