RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses
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RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses. / Grauel, M Katharina; Maglione, Marta; Reddy-Alla, Suneel; Willmes, Claudia G; Brockmann, Marisa M; Trimbuch, Thorsten; Rosenmund, Tanja; Pangalos, Maria; Vardar, Gülçin; Stumpf, Alexander; Walter, Alexander M; Rost, Benjamin R; Eickholt, Britta J; Haucke, Volker; Schmitz, Dietmar; Sigrist, Stephan J; Rosenmund, Christian.
In: P NATL ACAD SCI USA, Vol. 113, No. 41, 11.10.2016, p. 11615-11620.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses
AU - Grauel, M Katharina
AU - Maglione, Marta
AU - Reddy-Alla, Suneel
AU - Willmes, Claudia G
AU - Brockmann, Marisa M
AU - Trimbuch, Thorsten
AU - Rosenmund, Tanja
AU - Pangalos, Maria
AU - Vardar, Gülçin
AU - Stumpf, Alexander
AU - Walter, Alexander M
AU - Rost, Benjamin R
AU - Eickholt, Britta J
AU - Haucke, Volker
AU - Schmitz, Dietmar
AU - Sigrist, Stephan J
AU - Rosenmund, Christian
PY - 2016/10/11
Y1 - 2016/10/11
N2 - The tight spatial coupling of synaptic vesicles and voltage-gated Ca2+ channels (CaVs) ensures efficient action potential-triggered neurotransmitter release from presynaptic active zones (AZs). Rab-interacting molecule-binding proteins (RIM-BPs) interact with Ca2+ channels and via RIM with other components of the release machinery. Although human RIM-BPs have been implicated in autism spectrum disorders, little is known about the role of mammalian RIM-BPs in synaptic transmission. We investigated RIM-BP2-deficient murine hippocampal neurons in cultures and slices. Short-term facilitation is significantly enhanced in both model systems. Detailed analysis in culture revealed a reduction in initial release probability, which presumably underlies the increased short-term facilitation. Superresolution microscopy revealed an impairment in CaV2.1 clustering at AZs, which likely alters Ca2+ nanodomains at release sites and thereby affects release probability. Additional deletion of RIM-BP1 does not exacerbate the phenotype, indicating that RIM-BP2 is the dominating RIM-BP isoform at these synapses.
AB - The tight spatial coupling of synaptic vesicles and voltage-gated Ca2+ channels (CaVs) ensures efficient action potential-triggered neurotransmitter release from presynaptic active zones (AZs). Rab-interacting molecule-binding proteins (RIM-BPs) interact with Ca2+ channels and via RIM with other components of the release machinery. Although human RIM-BPs have been implicated in autism spectrum disorders, little is known about the role of mammalian RIM-BPs in synaptic transmission. We investigated RIM-BP2-deficient murine hippocampal neurons in cultures and slices. Short-term facilitation is significantly enhanced in both model systems. Detailed analysis in culture revealed a reduction in initial release probability, which presumably underlies the increased short-term facilitation. Superresolution microscopy revealed an impairment in CaV2.1 clustering at AZs, which likely alters Ca2+ nanodomains at release sites and thereby affects release probability. Additional deletion of RIM-BP1 does not exacerbate the phenotype, indicating that RIM-BP2 is the dominating RIM-BP isoform at these synapses.
KW - Action Potentials
KW - Animals
KW - Calcium/metabolism
KW - Calcium Channels/metabolism
KW - Cells, Cultured
KW - Electrophysiological Phenomena
KW - Female
KW - Gene Deletion
KW - Gene Expression
KW - Gene Targeting
KW - Genetic Loci
KW - Hippocampus/metabolism
KW - Male
KW - Mice
KW - Mice, Knockout
KW - Neurons/metabolism
KW - Phenotype
KW - Protein Transport
KW - Synapses/metabolism
KW - Synaptic Transmission/genetics
KW - Synaptic Vesicles/metabolism
U2 - 10.1073/pnas.1605256113
DO - 10.1073/pnas.1605256113
M3 - SCORING: Journal article
C2 - 27671655
VL - 113
SP - 11615
EP - 11620
JO - P NATL ACAD SCI USA
JF - P NATL ACAD SCI USA
SN - 0027-8424
IS - 41
ER -