Research ExplorerPublicationsRGB marking facilitates multicolor clonal cell tracking.

RGB marking facilitates multicolor clonal cell tracking.

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RGB marking facilitates multicolor clonal cell tracking. / Riecken, Kristoffer; Thomaschewski, Michael; Warlich, Michael; Volz, Tassilo; Cornils, Kerstin; Niebuhr, Birte; Täger, Maike; Lütgehetmann, Marc; Pollok, Jörg-Matthias; Stocking, Carol; Dandri-Petersen, Maura; Benten, Daniel; Fehse, Boris.

In: NAT MED, Vol. 17, No. 4, 4, 2011, p. 504-509.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Riecken, K, Thomaschewski, M, Warlich, M, Volz, T, Cornils, K, Niebuhr, B, Täger, M, Lütgehetmann, M, Pollok, J-M, Stocking, C, Dandri-Petersen, M, Benten, D & Fehse, B 2011, 'RGB marking facilitates multicolor clonal cell tracking.', NAT MED, vol. 17, no. 4, 4, pp. 504-509. <http://www.ncbi.nlm.nih.gov/pubmed/21441917?dopt=Citation>

APA

Riecken, K., Thomaschewski, M., Warlich, M., Volz, T., Cornils, K., Niebuhr, B., Täger, M., Lütgehetmann, M., Pollok, J-M., Stocking, C., Dandri-Petersen, M., Benten, D., & Fehse, B. (2011). RGB marking facilitates multicolor clonal cell tracking. NAT MED, 17(4), 504-509. [4]. http://www.ncbi.nlm.nih.gov/pubmed/21441917?dopt=Citation

Vancouver

Riecken K, Thomaschewski M, Warlich M, Volz T, Cornils K, Niebuhr B et al. RGB marking facilitates multicolor clonal cell tracking. NAT MED. 2011;17(4):504-509. 4.

Bibtex

@article{849c6c3ada4740c79ac7148091bc0e5e,
title = "RGB marking facilitates multicolor clonal cell tracking.",
abstract = "We simultaneously transduced cells with three lentiviral gene ontology (LeGO) vectors encoding red, green or blue fluorescent proteins. Individual cells were thereby marked by different combinations of inserted vectors, resulting in the generation of numerous mixed colors, a principle we named red-green-blue (RGB) marking. We show that lentiviral vector-mediated RGB marking remained stable after cell division, thus facilitating the analysis of clonal cell fates in vitro and in vivo. Particularly, we provide evidence that RGB marking allows assessment of clonality after regeneration of injured livers by transplanted primary hepatocytes. We also used RGB vectors to mark hematopoietic stem/progenitor cells that generated colored spleen colonies. Finally, based on limiting-dilution and serial transplantation assays with tumor cells, we found that clonal tumor cells retained their specific color-code over extensive periods of time. We conclude that RGB marking represents a useful tool for cell clonality studies in tissue regeneration and pathology.",
keywords = "Animals, Mice, Mice, Inbred C57BL, Mice, Transgenic, Transduction, Genetic, Genetic Vectors, Neoplasm Transplantation, Mice, SCID, Cell Tracking/*methods, Clone Cells/*cytology/*metabolism, Color, Green Fluorescent Proteins/genetics/metabolism, Hepatocytes/cytology/metabolism, Liver Regeneration, Luminescent Proteins/*genetics/*metabolism, Mice, Inbred NOD, Recombinant Proteins/genetics/metabolism, Tumor Cells, Cultured/metabolism/pathology, Animals, Mice, Mice, Inbred C57BL, Mice, Transgenic, Transduction, Genetic, Genetic Vectors, Neoplasm Transplantation, Mice, SCID, Cell Tracking/*methods, Clone Cells/*cytology/*metabolism, Color, Green Fluorescent Proteins/genetics/metabolism, Hepatocytes/cytology/metabolism, Liver Regeneration, Luminescent Proteins/*genetics/*metabolism, Mice, Inbred NOD, Recombinant Proteins/genetics/metabolism, Tumor Cells, Cultured/metabolism/pathology",
author = "Kristoffer Riecken and Michael Thomaschewski and Michael Warlich and Tassilo Volz and Kerstin Cornils and Birte Niebuhr and Maike T{\"a}ger and Marc L{\"u}tgehetmann and J{\"o}rg-Matthias Pollok and Carol Stocking and Maura Dandri-Petersen and Daniel Benten and Boris Fehse",
year = "2011",
language = "English",
volume = "17",
pages = "504--509",
journal = "NAT MED",
issn = "1078-8956",
publisher = "NATURE PUBLISHING GROUP",
number = "4",

}

RIS

TY - JOUR

T1 - RGB marking facilitates multicolor clonal cell tracking.

AU - Riecken, Kristoffer

AU - Thomaschewski, Michael

AU - Warlich, Michael

AU - Volz, Tassilo

AU - Cornils, Kerstin

AU - Niebuhr, Birte

AU - Täger, Maike

AU - Lütgehetmann, Marc

AU - Pollok, Jörg-Matthias

AU - Stocking, Carol

AU - Dandri-Petersen, Maura

AU - Benten, Daniel

AU - Fehse, Boris

PY - 2011

Y1 - 2011

N2 - We simultaneously transduced cells with three lentiviral gene ontology (LeGO) vectors encoding red, green or blue fluorescent proteins. Individual cells were thereby marked by different combinations of inserted vectors, resulting in the generation of numerous mixed colors, a principle we named red-green-blue (RGB) marking. We show that lentiviral vector-mediated RGB marking remained stable after cell division, thus facilitating the analysis of clonal cell fates in vitro and in vivo. Particularly, we provide evidence that RGB marking allows assessment of clonality after regeneration of injured livers by transplanted primary hepatocytes. We also used RGB vectors to mark hematopoietic stem/progenitor cells that generated colored spleen colonies. Finally, based on limiting-dilution and serial transplantation assays with tumor cells, we found that clonal tumor cells retained their specific color-code over extensive periods of time. We conclude that RGB marking represents a useful tool for cell clonality studies in tissue regeneration and pathology.

AB - We simultaneously transduced cells with three lentiviral gene ontology (LeGO) vectors encoding red, green or blue fluorescent proteins. Individual cells were thereby marked by different combinations of inserted vectors, resulting in the generation of numerous mixed colors, a principle we named red-green-blue (RGB) marking. We show that lentiviral vector-mediated RGB marking remained stable after cell division, thus facilitating the analysis of clonal cell fates in vitro and in vivo. Particularly, we provide evidence that RGB marking allows assessment of clonality after regeneration of injured livers by transplanted primary hepatocytes. We also used RGB vectors to mark hematopoietic stem/progenitor cells that generated colored spleen colonies. Finally, based on limiting-dilution and serial transplantation assays with tumor cells, we found that clonal tumor cells retained their specific color-code over extensive periods of time. We conclude that RGB marking represents a useful tool for cell clonality studies in tissue regeneration and pathology.

KW - Animals

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Transduction, Genetic

KW - Genetic Vectors

KW - Neoplasm Transplantation

KW - Mice, SCID

KW - Cell Tracking/methods

KW - Clone Cells/cytology/metabolism

KW - Color

KW - Green Fluorescent Proteins/genetics/metabolism

KW - Hepatocytes/cytology/metabolism

KW - Liver Regeneration

KW - Luminescent Proteins/genetics/metabolism

KW - Mice, Inbred NOD

KW - Recombinant Proteins/genetics/metabolism

KW - Tumor Cells, Cultured/metabolism/pathology

KW - Animals

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Transduction, Genetic

KW - Genetic Vectors

KW - Neoplasm Transplantation

KW - Mice, SCID

KW - Cell Tracking/methods

KW - Clone Cells/cytology/metabolism

KW - Color

KW - Green Fluorescent Proteins/genetics/metabolism

KW - Hepatocytes/cytology/metabolism

KW - Liver Regeneration

KW - Luminescent Proteins/genetics/metabolism

KW - Mice, Inbred NOD

KW - Recombinant Proteins/genetics/metabolism

KW - Tumor Cells, Cultured/metabolism/pathology

M3 - SCORING: Journal article

VL - 17

SP - 504

EP - 509

JO - NAT MED

JF - NAT MED

SN - 1078-8956

IS - 4

M1 - 4

ER -