Revolutionizing membrane protein overexpression in bacteria

Susan Schlegel; Mirjam Klepsch; Dimitra Gialama; David Wickström; Dirk Jan Slotboom; Jan-Willem de Gier

doi:10.1111/j.1751-7915.2009.00148.x

Revolutionizing membrane protein overexpression in bacteria

Standard

Revolutionizing membrane protein overexpression in bacteria. / Schlegel, Susan; Klepsch, Mirjam; Gialama, Dimitra; Wickström, David; Slotboom, Dirk Jan; de Gier, Jan-Willem.

In: MICROB BIOTECHNOL, Vol. 3, No. 4, 07.2010, p. 403-11.

Research output: SCORING: Contribution to journal › SCORING: Review article › Research

Harvard

Schlegel, S, Klepsch, M, Gialama, D, Wickström, D, Slotboom, DJ & de Gier, J-W 2010, 'Revolutionizing membrane protein overexpression in bacteria', MICROB BIOTECHNOL, vol. 3, no. 4, pp. 403-11. https://doi.org/10.1111/j.1751-7915.2009.00148.x

APA

Schlegel, S., Klepsch, M., Gialama, D., Wickström, D., Slotboom, D. J., & de Gier, J-W. (2010). Revolutionizing membrane protein overexpression in bacteria. MICROB BIOTECHNOL, 3(4), 403-11. https://doi.org/10.1111/j.1751-7915.2009.00148.x

Vancouver

Schlegel S, Klepsch M, Gialama D, Wickström D, Slotboom DJ, de Gier J-W. Revolutionizing membrane protein overexpression in bacteria. MICROB BIOTECHNOL. 2010 Jul;3(4):403-11. https://doi.org/10.1111/j.1751-7915.2009.00148.x

Bibtex

@article{4a76d2d6d781405d9007b0c7712f1831,

title = "Revolutionizing membrane protein overexpression in bacteria",

abstract = "The bacterium Escherichia coli is the most widely used expression host for overexpression trials of membrane proteins. Usually, different strains, culture conditions and expression regimes are screened for to identify the optimal overexpression strategy. However, yields are often not satisfactory, especially for eukaryotic membrane proteins. This has initiated a revolution of membrane protein overexpression in bacteria. Recent studies have shown that it is feasible to (i) engineer or select for E. coli strains with strongly improved membrane protein overexpression characteristics, (ii) use bacteria other than E. coli for the expression of membrane proteins, (iii) engineer or select for membrane protein variants that retain functionality but express better than the wild-type protein, and (iv) express membrane proteins using E. coli-based cell-free systems.",

keywords = "Bacteria, Biotechnology, Cell-Free System, Gene Expression, Genetic Engineering, Membrane Proteins, Recombinant Proteins, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review",

author = "Susan Schlegel and Mirjam Klepsch and Dimitra Gialama and David Wickstr{\"o}m and Slotboom, {Dirk Jan} and {de Gier}, Jan-Willem",

note = "{\textcopyright} 2009 The Authors. Journal compilation {\textcopyright} 2009 Society for Applied Microbiology and Blackwell Publishing Ltd.",

year = "2010",

month = jul,

doi = "10.1111/j.1751-7915.2009.00148.x",

language = "English",

volume = "3",

pages = "403--11",

journal = "MICROB BIOTECHNOL",

issn = "1751-7915",

publisher = "John Wiley and Sons Ltd",

number = "4",

}

RIS

TY - JOUR

T1 - Revolutionizing membrane protein overexpression in bacteria

AU - Schlegel, Susan

AU - Klepsch, Mirjam

AU - Gialama, Dimitra

AU - Wickström, David

AU - Slotboom, Dirk Jan

AU - de Gier, Jan-Willem

PY - 2010/7

Y1 - 2010/7

N2 - The bacterium Escherichia coli is the most widely used expression host for overexpression trials of membrane proteins. Usually, different strains, culture conditions and expression regimes are screened for to identify the optimal overexpression strategy. However, yields are often not satisfactory, especially for eukaryotic membrane proteins. This has initiated a revolution of membrane protein overexpression in bacteria. Recent studies have shown that it is feasible to (i) engineer or select for E. coli strains with strongly improved membrane protein overexpression characteristics, (ii) use bacteria other than E. coli for the expression of membrane proteins, (iii) engineer or select for membrane protein variants that retain functionality but express better than the wild-type protein, and (iv) express membrane proteins using E. coli-based cell-free systems.

AB - The bacterium Escherichia coli is the most widely used expression host for overexpression trials of membrane proteins. Usually, different strains, culture conditions and expression regimes are screened for to identify the optimal overexpression strategy. However, yields are often not satisfactory, especially for eukaryotic membrane proteins. This has initiated a revolution of membrane protein overexpression in bacteria. Recent studies have shown that it is feasible to (i) engineer or select for E. coli strains with strongly improved membrane protein overexpression characteristics, (ii) use bacteria other than E. coli for the expression of membrane proteins, (iii) engineer or select for membrane protein variants that retain functionality but express better than the wild-type protein, and (iv) express membrane proteins using E. coli-based cell-free systems.

KW - Bacteria

KW - Biotechnology

KW - Cell-Free System

KW - Gene Expression

KW - Genetic Engineering

KW - Membrane Proteins

KW - Recombinant Proteins

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

KW - Review

U2 - 10.1111/j.1751-7915.2009.00148.x

DO - 10.1111/j.1751-7915.2009.00148.x

M3 - SCORING: Review article

C2 - 21255339

VL - 3

SP - 403

EP - 411

JO - MICROB BIOTECHNOL

JF - MICROB BIOTECHNOL

SN - 1751-7915

IS - 4

ER -